##################################
# NO CUT ENZYMES (  222 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstNI       BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DpnII       
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRII      EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HphI        KasI        
KpnI        Ksp632I     KspI        MaeI        MaeIII      
MamI        MboI        MflI        MluI        MluNI       
MroI        MscI        MseI        MslI        MvaI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NlaIII      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      Sau96I      SbfI        ScaI        
ScrFI       SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        Tru9I       
Tsp45I      TspRI       Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AciI          102  CCGC                 CCGC(-3/-1)
AhdI           55  GACGTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI          135  AGCT                 AG^CT
AspEI          55  GACGTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvI          136  GCTGC                GCAGC(8/12)
BpmI          172  CTGGAG               CTGGAG(16/14)
BsiYI         165  CCTCAAACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          165  CCTCAAACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI          108  GAATGC               GAATGC(1/-1)
BsrI          171  ACTGG                ACTGG(1/-1)
BstF5I        123  CATCC                GGATG(2/0)
Cac8I          97  GCAGGC               GC[ACGT]^[ACGT]GC
DdeI          153  CTCAG                C^T[ACGT]AG
Eam1105I       55  GACGTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FokI          123  CATCC                GGATG(9/13)
HaeIII        100  GGCC                 GG^CC
HapII         105  CCGG                 C^CGG
HpaII         105  CCGG                 C^CGG
MaeII          56  ACGT                 A^CGT
MfeI          232  CAATTG               C^AATTG
MspA1I        134  CAGCTG               C[AC]G^C[GT]G
MspI          105  CCGG                 C^CGG
MunI          232  CAATTG               C^AATTG
NlaIV         148  GGAACC               GG[ACGT]^[ACGT]CC
PleI           61  GAGTC                GAGTC(4/5)
PvuII         134  CAGCTG               CAG^CTG
TfiI           37  GATTC                G^A[AT]TC
TseI          136  GCTGC                G^C[AT]GC


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

Fnu4HI        101  GCCGC                GC^[ACGT]GC
              136  GCTGC               
HinfI          37  GATTC                G^A[ACGT]TC
               61  GAGTC               
ItaI          101  GCCGC                GC^[ACGT]GC
              136  GCTGC               
MwoI           73  GCCAATTTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
               82  GCATCTTCAGC         
SfaNI         196  GATGC                GCATC(5/9)
               82  GCATC               
Tsp509I        76  AATT                 ^AATT
              233  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    2 enzymes) #
#########################################

MboII       MnlI