##################################
# NO CUT ENZYMES (  207 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbsI        BbvCI       BciVI       BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BpuAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DdeI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FseI        
FspI        HgaI        Hin1I       HincII      HindII      
HindIII     HinfI       HpaI        HphI        KasI        
KpnI        Ksp632I     KspI        MaeI        MaeIII      
MamI        MboII       MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MspA1I      MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NlaIII      NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmeI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TfiI        
Tru9I       Tsp45I      TspRI       Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   22 enzymes) #
###################################

AciI          135  CCGC                 CCGC(-3/-1)
AluI          173  AGCT                 AG^CT
Aor51HI       122  AGCGCT               AGC^GCT
AvaII         159  GGACC                G^G[AT]CC
BbrPI         154  CACGTG               CAC^GTG
BclI            2  TGATCA               T^GATCA
BpmI          193  CTGGAG               CTGGAG(16/14)
BsaAI         154  CACGTG               [CT]AC^GT[GA]
Eco47III      122  AGCGCT               AGC^GCT
FbaI            2  TGATCA               T^GATCA
HaeII         122  AGCGCT               [GA]GCGC^[CT]
HapII         138  CCGG                 C^CGG
HpaII         138  CCGG                 C^CGG
MaeII         155  ACGT                 A^CGT
MspI          138  CCGG                 C^CGG
PmaCI         154  CACGTG               CAC^GTG
PmlI          154  CACGTG               CAC^GTG
PstI           73  CTGCAG               CTGCA^G
SfcI           73  CTGCAG               C^T[GA][CT]AG
TaqI          167  TCGA                 T^CGA
Tsp509I       181  AATT                 ^AATT
TthHB8I       167  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AfaI           13  GTAC                 GT^AC
               84  GTAC                
BcgI           90  CGAGGGGGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
               32  GCAAGATCGTCG        
Cac8I          75  GCAGGC               GC[ACGT]^[ACGT]GC
              147  GCTGGC              
CfoI          100  GCGC                 GCG^C
              123  GCGC                
HhaI          100  GCGC                 GCG^C
              123  GCGC                
HinP1I        100  GCGC                 G^CGC
              123  GCGC                
MnlI           55  GAGG                 CCTC(7/6)
               91  GAGG                
RsaI           13  GTAC                 GT^AC
               84  GTAC                


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

BbvI        BsaJI       BssKI       BstF5I      BstNI       
Cfr13I      DpnII       EcoRII      Fnu4HI      FokI        
HaeIII      ItaI        MboI        MvaI        MwoI        
NdeII       Sau3AI      Sau96I      ScrFI       SfaNI       
TseI