Restriction map for
pF1KB8841
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BbeI BbrPI BbsI BbvCI
BcgI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoT22I FbaI FseI FspI
HaeII HhaI HinP1I HincII HindII
HpaI KasI KpnI KspI MaeII
MluI MluNI MroI MscI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
SspBI SwaI TaqI Tsp45I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AcyI 345 GGCGTC G[GA]^CG[CT]C
BsaBI 1189 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 345 GGCGTC G[GA]^CG[CT]C
BsgI 67 GTGCAG GTGCAG(16/14)
BsmBI 347 CGTCTC CGTCTC(1/5)
DraIII 1158 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1232 GACCCGAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1177 GAAGAG CTCTTC(1/4)
EcoRV 886 GATATC GAT^ATC
FauI 1402 GCGGG CCCGC(4/6)
HapII 360 CCGG C^CGG
HgaI 346 GCGTC GACGC(5/10)
Hin1I 345 GGCGTC G[GA]^CG[CT]C
HindIII 748 AAGCTT A^AGCTT
HpaII 360 CCGG C^CGG
HphI 825 GGTGA GGTGA(8/7)
Ksp632I 1177 GAAGAG CTCTTC(1/4)
MaeIII 779 GTTAC ^GT[ACGT]AC
MamI 1189 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 840 CAATTG C^AATTG
MseI 724 TTAA T^TAA
MspI 360 CCGG C^CGG
MunI 840 CAATTG C^AATTG
SbfI 1395 CCTGCAGG CCTGCA^GG
SmaI 359 CCCGGG CCC^GGG
Sse8387I 1395 CCTGCAGG CCTGCA^GG
SspI 487 AATATT AAT^ATT
StuI 1415 AGGCCT AGG^CCT
Tru9I 724 TTAA T^TAA
XmaCI 359 CCCGGG C^CCGGG
XmaI 359 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AciI 1326 CCGC CCGC(-3/-1)
1402 GCGG
AlwNI 70 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1390 CAGGCCCTG
AspHI 196 GAGCAC G[AT]GC[AT]^C
931 GTGCTC
AvaI 359 CCCGGG C^[CT]CG[GA]G
1234 CCCGAG
AvaII 1231 GGACC G^G[AT]CC
1308 GGTCC
BamHI 477 GGATCC G^GATCC
1073 GGATCC
BanI 507 GGCACC G^G[CT][GA]CC
797 GGCACC
BanII 178 GAGCCC G[GA]GC[CT]^C
454 GAGCCC
BciVI 939 GGATAC GTATCC(6/5)
804 GTATCC
BcnI 359 CCCGG CC^[GC]GG
360 CCGGG
BfaI 538 CTAG C^TAG
601 CTAG
BglI 217 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1393 GCCCTGCAGGC
BsiHKAI 196 GAGCAC G[AT]GC[AT]^C
931 GTGCTC
BsoBI 359 CCCGGG C^[CT]CG[GA]G
1234 CCCGAG
BsrDI 1035 CATTGC GCAATG(2/0)
909 GCAATG
BstXI 211 CCAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
298 CCAGCAGCCTGG
EcoRI 462 GAATTC G^AATTC
595 GAATTC
MaeI 538 CTAG C^TAG
601 CTAG
MslI 924 CATACCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1288 CACATCAGTG
MspA1I 769 CAGCTG C[AC]G^C[GT]G
1247 CAGCTG
NciI 359 CCCGG CC^[GC]GG
360 CCGGG
PvuII 769 CAGCTG CAG^CTG
1247 CAGCTG
TfiI 204 GAATC G^A[AT]TC
739 GATTC
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbvI
BmyI BpmI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
ItaI MboI MboII MflI MnlI
MvaI MwoI NdeII NlaIII NlaIV
PleI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TseI
Tsp509I TspRI XhoII