Restriction map for
pF1KB8840
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflIII AgeI AhdI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BbeI
BbrPI BbsI BcgI BciVI BclI
BlnI BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MamI
MfeI MluI MroI MslI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SspBI SspI StuI SwaI TfiI
Tsp509I Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AccI 505 GTCTAC GT^[AC][GT]AC
AfaI 1045 GTAC GT^AC
AflII 553 CTTAAG C^TTAAG
Alw44I 37 GTGCAC G^TGCAC
AocI 1234 CCTAAGG CC^T[ACGT]AGG
ApaLI 37 GTGCAC G^TGCAC
AviII 194 TGCGCA TGC^GCA
BamHI 1337 GGATCC G^GATCC
BbvCI 1177 GCTGAGG CCTCAGC(-5/-2)
BfaI 1246 CTAG C^TAG
BfrI 553 CTTAAG C^TTAAG
BglII 1191 AGATCT A^GATCT
BsgI 467 GTGCAG GTGCAG(16/14)
BsmAI 1150 GAGAC GTCTC(1/5)
BsmFI 789 GTCCC GGGAC(10/14)
BsrDI 1379 CATTGC GCAATG(2/0)
BstAPI 156 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 998 CCAAAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1234 CCTAAGG CC^T[ACGT]AGG
DsaI 59 CCACGG C^C[GA][CT]GG
Eco81I 1234 CCTAAGG CC^T[ACGT]AGG
EcoNI 78 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 1326 GCGGG CCCGC(4/6)
FspI 194 TGCGCA TGC^GCA
MaeI 1246 CTAG C^TAG
PflMI 138 CCAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 787 GGGTCCC [GA]G^G[AT]CC[CT]
RsaI 1045 GTAC GT^AC
SacI 429 GAGCTC GAGCT^C
SphI 405 GCATGC GCATG^C
Van91I 138 CCAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 50 CGCG CG^CG
1345 CGCG
AlwNI 157 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
595 CAGTCACTG
ApaI 702 GGGCCC GGGCC^C
1222 GGGCCC
AvaI 461 CCCGGG C^[CT]CG[GA]G
926 CCCGGG
BalI 374 TGGCCA TGG^CCA
866 TGGCCA
BlpI 426 GCTGAGC GC^T[ACGT]AGC
1330 GCTCAGC
BpmI 293 CTCCAG CTGGAG(16/14)
1372 CTCCAG
Bpu1102I 426 GCTGAGC GC^T[ACGT]AGC
1330 GCTCAGC
BseRI 623 CTCCTC GAGGAG(10/8)
1180 GAGGAG
BsoBI 461 CCCGGG C^[CT]CG[GA]G
926 CCCGGG
BspMI 419 ACCTGC ACCTGC(4/8)
331 GCAGGT
BssSI 263 CTCGTG CACGAG(-5/-1)
878 CTCGTG
BstUI 50 CGCG CG^CG
1345 CGCG
CelII 426 GCTGAGC GC^T[ACGT]AGC
1330 GCTCAGC
CfoI 51 GCGC GCG^C
195 GCGC
EaeI 374 TGGCCA [CT]^GGCC[GA]
866 TGGCCA
HhaI 51 GCGC GCG^C
195 GCGC
HinP1I 51 GCGC G^CGC
195 GCGC
MaeIII 597 GTCAC ^GT[ACGT]AC
1356 GTCAC
MluNI 374 TGGCCA TGG^CCA
866 TGGCCA
MscI 374 TGGCCA TGG^CCA
866 TGGCCA
MseI 101 TTAA T^TAA
554 TTAA
MspA1I 1324 CAGCGG C[AC]G^C[GT]G
1333 CAGCGG
MvnI 50 CGCG CG^CG
1345 CGCG
NspI 18 GCATGT [GA]CATG^[CT]
405 GCATGC
SbfI 153 CCTGCAGG CCTGCA^GG
328 CCTGCAGG
SfaNI 53 GCATC GCATC(5/9)
382 GCATC
SmaI 461 CCCGGG CCC^GGG
926 CCCGGG
SmlI 535 CTTGAG C^T[CT][GA]AG
553 CTTAAG
Sse8387I 153 CCTGCAGG CCTGCA^GG
328 CCTGCAGG
TaqI 858 TCGA T^CGA
900 TCGA
Tru9I 101 TTAA T^TAA
554 TTAA
Tsp45I 597 GTCAC ^GT[GC]AC
1356 GTCAC
TthHB8I 858 TCGA T^CGA
900 TCGA
XmaCI 461 CCCGGG C^CCGGG
926 CCCGGG
XmaI 461 CCCGGG C^CCGGG
926 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BanI BanII BbvI BcnI BglI
BmyI BsaJI BsiHKAI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MboI
MboII MflI MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI Sau3AI Sau96I ScrFI
SfcI StyI TseI TspRI XcmI
XhoII