Restriction map for
pF1KB8839
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI AlwNI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BbeI BbrPI BbvCI BcgI
BciVI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmAI BsmBI BsmI BspDI
BspEI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT14I FseI FspI HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MaeII MamI MluI MluNI
MroI MscI MvnI NaeI NarI
NcoI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I StuI StyI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Alw44I 1238 GTGCAC G^TGCAC
ApaI 1199 GGGCCC GGGCC^C
ApaLI 1238 GTGCAC G^TGCAC
BanI 982 GGCACC G^G[CT][GA]CC
BclI 596 TGATCA T^GATCA
BsmFI 273 GGGAC GGGAC(10/14)
Bsp1407I 236 TGTACA T^GTACA
BspHI 602 TCATGA T^CATGA
BspMI 418 GCAGGT ACCTGC(4/8)
BsrGI 236 TGTACA T^GTACA
BstEII 105 GGTGACC G^GT[ACGT]ACC
BstPI 105 GGTGACC G^GT[ACGT]ACC
CfoI 1388 GCGC GCG^C
DraI 1351 TTTAAA TTT^AAA
DraIII 1257 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 307 GACTATGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1028 CCGTGG C^C[GA][CT]GG
EarI 478 CTCTTC CTCTTC(1/4)
EcoO65I 105 GGTGACC G^GT[ACGT]ACC
EcoT22I 969 ATGCAT ATGCA^T
FauI 1085 CCCGC CCCGC(4/6)
FbaI 596 TGATCA T^GATCA
HaeII 1387 GGCGCT [GA]GCGC^[CT]
HapII 1140 CCGG C^CGG
HhaI 1388 GCGC GCG^C
HinP1I 1388 GCGC G^CGC
HpaII 1140 CCGG C^CGG
Ksp632I 478 CTCTTC CTCTTC(1/4)
MfeI 1268 CAATTG C^AATTG
MspA1I 434 CAGCTG C[AC]G^C[GT]G
MspI 1140 CCGG C^CGG
MunI 1268 CAATTG C^AATTG
NheI 297 GCTAGC G^CTAGC
NsiI 969 ATGCAT ATGCA^T
PvuII 434 CAGCTG CAG^CTG
RcaI 602 TCATGA T^CATGA
SacI 843 GAGCTC GAGCT^C
SmaI 1139 CCCGGG CCC^GGG
SspBI 236 TGTACA T^GTACA
SspI 1075 AATATT AAT^ATT
TfiI 336 GAATC G^A[AT]TC
XcmI 1157 CCAGGACAATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1139 CCCGGG C^CCGGG
XmaI 1139 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaI 629 CCCGAG C^[CT]CG[GA]G
1139 CCCGGG
BamHI 91 GGATCC G^GATCC
801 GGATCC
BbsI 1344 GAAGAC GAAGAC(2/6)
1044 GTCTTC
BcnI 1139 CCCGG CC^[GC]GG
1140 CCGGG
BfaI 127 CTAG C^TAG
298 CTAG
BpuAI 1344 GAAGAC GAAGAC(2/6)
1044 GTCTTC
BsoBI 629 CCCGAG C^[CT]CG[GA]G
1139 CCCGGG
BsrDI 320 GCAATG GCAATG(2/0)
1328 GCAATG
BstXI 407 CCACAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
926 CCACTTCCCTGG
BstYI 91 GGATCC [GA]^GATC[CT]
801 GGATCC
HincII 148 GTCAAC GT[CT]^[GA]AC
169 GTCAAC
HindII 148 GTCAAC GT[CT]^[GA]AC
169 GTCAAC
MaeI 127 CTAG C^TAG
298 CTAG
MflI 91 GGATCC [GA]^GATC[CT]
801 GGATCC
MslI 408 CACAAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
603 CATGACTGTG
NciI 1139 CCCGG CC^[GC]GG
1140 CCGGG
NspI 740 ACATGC [GA]CATG^[CT]
967 ACATGC
PleI 853 GACTC GAGTC(4/5)
1003 GACTC
PpuMI 273 GGGACCT [GA]G^G[AT]CC[CT]
443 GGGTCCT
SfaNI 971 GCATC GCATC(5/9)
1247 GCATC
XhoII 91 GGATCC [GA]^GATC[CT]
801 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanII BbvI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI Tru9I TseI Tsp45I
Tsp509I TspRI