Restriction map for
pF1KB8836
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BcnI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT14I
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KpnI Ksp632I KspI MaeII
MfeI MluI MluNI MroI MscI
MspA1I MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspV PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI StuI StyI SwaI
TaqI Tsp45I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AflII 352 CTTAAG C^TTAAG
AlwI 425 GATCC GGATC(4/5)
BbvI 320 GCAGC GCAGC(8/12)
BclI 1236 TGATCA T^GATCA
BfaI 31 CTAG C^TAG
BfrI 352 CTTAAG C^TTAAG
BglII 299 AGATCT A^GATCT
BsaBI 76 GATAGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 41 CCTGGG C^C[ACGT][ACGT]GG
BsgI 318 GTGCAG GTGCAG(16/14)
BsiYI 1337 CCTTCAGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1337 CCTTCAGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 951 GAGAC GTCTC(1/5)
BsmFI 66 GGGAC GGGAC(10/14)
BsrDI 1151 GCAATG GCAATG(2/0)
BssKI 41 CCTGG ^CC[ACGT]GG
BstNI 41 CCTGG CC^[AT]GG
BstYI 299 AGATCT [GA]^GATC[CT]
Cac8I 761 GCATGC GC[ACGT]^[ACGT]GC
Cfr13I 215 GGCCC G^G[ACGT]CC
DraII 214 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 214 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRII 41 CCTGG ^CC[AT]GG
EcoT22I 763 ATGCAT ATGCA^T
FauI 908 GCGGG CCCGC(4/6)
FbaI 1236 TGATCA T^GATCA
Fnu4HI 320 GCAGC GC^[ACGT]GC
HaeIII 215 GGCC GG^CC
HapII 1344 CCGG C^CGG
HpaII 1344 CCGG C^CGG
HphI 1216 GGTGA GGTGA(8/7)
ItaI 320 GCAGC GC^[ACGT]GC
MaeI 31 CTAG C^TAG
MamI 76 GATAGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 299 AGATCT [GA]^GATC[CT]
MspI 1344 CCGG C^CGG
MvaI 41 CCTGG CC^[AT]GG
NsiI 763 ATGCAT ATGCA^T
NspI 761 GCATGC [GA]CATG^[CT]
PacI 265 TTAATTAA TTAAT^TAA
Sau96I 215 GGCCC G^G[ACGT]CC
ScrFI 41 CCTGG CC^[ACGT]GG
SmlI 352 CTTAAG C^T[CT][GA]AG
SphI 761 GCATGC GCATG^C
TseI 320 GCAGC G^C[AT]GC
XhoII 299 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
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AciI 908 GCGG CCGC(-3/-1)
1328 GCGG
Asp700I 408 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
613 GAAAGGATTC
AspHI 339 GAGCTC G[AT]GC[AT]^C
843 GAGCTC
BanII 339 GAGCTC G[GA]GC[CT]^C
843 GAGCTC
BmyI 339 GAGCTC G[AGT]GC[ACT]^C
843 GAGCTC
BsiHKAI 339 GAGCTC G[AT]GC[AT]^C
843 GAGCTC
Bsp1286I 339 GAGCTC G[AGT]GC[ACT]^C
843 GAGCTC
Bsp1407I 438 TGTACA T^GTACA
774 TGTACA
BsrGI 438 TGTACA T^GTACA
774 TGTACA
HindIII 662 AAGCTT A^AGCTT
998 AAGCTT
MboII 241 GAAGA GAAGA(8/7)
297 GAAGA
NlaIV 45 GGAACC GG[ACGT]^[ACGT]CC
215 GGCCCC
SacI 339 GAGCTC GAGCT^C
843 GAGCTC
SspBI 438 TGTACA T^GTACA
774 TGTACA
XmnI 408 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
613 GAAAGGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AcsI AfaI AluI ApoI BpmI
BsmI BsrI BstF5I DdeI DpnII
EcoRI FokI HinfI MaeIII MboI
MnlI MseI MslI NdeII NlaIII
RsaI Sau3AI SfaNI TfiI Tru9I
Tsp509I TspRI