Restriction map for
pF1KB8835
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflII AgeI AhdI AlwI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DpnII
DraI DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HgaI HindIII HpaI KpnI
KspI MamI MboI MfeI MflI
MluI MroI MunI NaeI NdeI
NdeII NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI Sau3AI SbfI ScaI SexAI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccII 1235 CGCG CG^CG
AcsI 280 GAATTC [GA]^AATT[CT]
AcyI 271 GGCGCC G[GA]^CG[CT]C
AflIII 611 ACGTGT A^C[GA][CT]GT
AlwNI 181 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 280 GAATTC [GA]^AATT[CT]
AspI 295 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 382 GAGCCC G[GA]GC[CT]^C
BbeI 271 GGCGCC GGCGC^C
BbrPI 340 CACGTG CAC^GTG
BbvCI 366 CCTCAGC CCTCAGC(-5/-2)
BfaI 422 CTAG C^TAG
BsaAI 340 CACGTG [CT]AC^GT[GA]
BsaHI 271 GGCGCC G[GA]^CG[CT]C
BsaI 213 GGTCTC GGTCTC(1/5)
BsgI 1222 CTGCAC GTGCAG(16/14)
BsiEI 1325 CGGCCG CG[GA][CT]^CG
BsmAI 214 GTCTC GTCTC(1/5)
BsmI 360 GAATGC GAATGC(1/-1)
BsrBI 88 GAGCGG CCGCTC(-3/-3)
BsrFI 662 ACCGGC [GA]^CCGG[CT]
BstAPI 1004 GCACCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1235 CGCG CG^CG
Cfr10I 662 ACCGGC [GA]^CCGG[CT]
EagI 1325 CGGCCG C^GGCCG
EarI 586 GAAGAG CTCTTC(1/4)
EclXI 1325 CGGCCG C^GGCCG
Eco52I 1325 CGGCCG C^GGCCG
EcoNI 396 CCTCAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 280 GAATTC G^AATTC
Hin1I 271 GGCGCC G[GA]^CG[CT]C
HincII 301 GTCAAC GT[CT]^[GA]AC
HindII 301 GTCAAC GT[CT]^[GA]AC
KasI 271 GGCGCC G^GCGCC
Ksp632I 586 GAAGAG CTCTTC(1/4)
MaeI 422 CTAG C^TAG
MvnI 1235 CGCG CG^CG
NarI 271 GGCGCC GG^CGCC
NcoI 902 CCATGG C^CATGG
NheI 421 GCTAGC G^CTAGC
NspI 1052 ACATGC [GA]CATG^[CT]
PflFI 295 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1026 CCAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 640 GAGTC GAGTC(4/5)
PmaCI 340 CACGTG CAC^GTG
PmlI 340 CACGTG CAC^GTG
PvuII 31 CAGCTG CAG^CTG
SfiI 1344 GGCCAGTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 271 GGCGCC GGC^GCC
SmaI 911 CCCGGG CCC^GGG
TaqI 132 TCGA T^CGA
TfiI 840 GATTC G^A[AT]TC
Tsp509I 281 AATT ^AATT
Tth111I 295 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 132 TCGA T^CGA
Van91I 1026 CCAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 869 CCACCTCGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 911 CCCGGG C^CCGGG
XmaI 911 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Alw44I 230 GTGCAC G^TGCAC
1002 GTGCAC
ApaLI 230 GTGCAC G^TGCAC
1002 GTGCAC
BalI 720 TGGCCA TGG^CCA
899 TGGCCA
BanI 271 GGCGCC G^G[CT][GA]CC
1295 GGCACC
BbsI 562 GAAGAC GAAGAC(2/6)
1045 GTCTTC
BglI 910 GCCCGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1345 GCCAGTGTGGC
BpuAI 562 GAAGAC GAAGAC(2/6)
1045 GTCTTC
BssSI 43 CACGAG CACGAG(-5/-1)
1161 CTCGTG
DdeI 367 CTCAG C^T[ACGT]AG
972 CTCAG
DraIII 340 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1005 CACCAAGTG
HaeII 271 GGCGCC [GA]GCGC^[CT]
442 AGCGCC
HinfI 640 GAGTC G^A[ACGT]TC
840 GATTC
HphI 786 GGTGA GGTGA(8/7)
163 TCACC
MaeIII 103 GTGAC ^GT[ACGT]AC
1155 GTCAC
MluNI 720 TGGCCA TGG^CCA
899 TGGCCA
MscI 720 TGGCCA TGG^CCA
899 TGGCCA
MseI 988 TTAA T^TAA
1361 TTAA
PpuMI 472 AGGACCC [GA]G^G[AT]CC[CT]
951 GGGACCC
PstI 208 CTGCAG CTGCA^G
328 CTGCAG
SmlI 124 CTCAAG C^T[CT][GA]AG
1300 CTCAAG
Tru9I 988 TTAA T^TAA
1361 TTAA
Tsp45I 103 GTGAC ^GT[GC]AC
1155 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI AspHI AvaI AvaII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII ItaI
MaeII MboII MnlI MslI MspA1I
MspI MvaI MwoI NciI NlaIII
NlaIV Sau96I ScrFI SfaNI SfcI
StuI StyI TseI TspRI