Restriction map for
pF1KB8833
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII AccII AclI AcyI AflII
AflIII AgeI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI AspI
AviII AvrII BalI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsiEI BsiWI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
BstXI CelII CfoI Cfr10I ClaI
CpoI DraI DraIII EagI EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
KasI KspI MamI MfeI MluI
MluNI MscI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TspRI
Tth111I Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 388 GGTACC G^GTACC
AccIII 560 TCCGGA T^CCGGA
AcsI 1231 GAATTC [GA]^AATT[CT]
AhdI 121 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 191 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 232 CCTGAGG CC^T[ACGT]AGG
ApoI 1231 GAATTC [GA]^AATT[CT]
Asp700I 1227 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 388 GGTACC G^GTACC
AspEI 121 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 289 GGATCC G^GATCC
BbsI 118 GAAGAC GAAGAC(2/6)
BglI 431 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 118 GAAGAC GAAGAC(2/6)
BsaAI 728 TACGTG [CT]AC^GT[GA]
BsaWI 560 TCCGGA [AT]^CCGG[AT]
BseAI 560 TCCGGA T^CCGGA
BseRI 124 GAGGAG GAGGAG(10/8)
BsgI 910 GTGCAG GTGCAG(16/14)
BsmBI 92 GAGACG CGTCTC(1/5)
BsmI 720 GAATGC GAATGC(1/-1)
BspEI 560 TCCGGA T^CCGGA
BsrI 78 ACTGG ACTGG(1/-1)
Bst1107I 70 GTATAC GTA^TAC
BstZ17I 70 GTATAC GTA^TAC
Bsu36I 232 CCTGAGG CC^T[ACGT]AGG
DrdI 880 GACACCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 884 CCATGG C^C[GA][CT]GG
EaeI 29 TGGCCG [CT]^GGCC[GA]
Eam1105I 121 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 89 GAAGAG CTCTTC(1/4)
Eco81I 232 CCTGAGG CC^T[ACGT]AGG
EcoNI 618 CCTCCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1231 GAATTC G^AATTC
HincII 933 GTTAAC GT[CT]^[GA]AC
HindII 933 GTTAAC GT[CT]^[GA]AC
HpaI 933 GTTAAC GTT^AAC
KpnI 388 GGTACC GGTAC^C
Ksp632I 89 GAAGAG CTCTTC(1/4)
MroI 560 TCCGGA T^CCGGA
MslI 942 CATGACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 884 CCATGG C^CATGG
PaeR7I 250 CTCGAG C^TCGAG
PvuII 905 CAGCTG CAG^CTG
SfaNI 1216 GCATC GCATC(5/9)
TaqI 251 TCGA T^CGA
TthHB8I 251 TCGA T^CGA
XhoI 250 CTCGAG C^TCGAG
XmnI 1227 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 70 GTATAC GT^[AC][GT]AC
129 GTCTAC
AspHI 528 GAGCAC G[AT]GC[AT]^C
608 GTGCTC
AvaI 11 CTCGGG C^[CT]CG[GA]G
250 CTCGAG
AvaII 407 GGACC G^G[AT]CC
627 GGTCC
BanI 55 GGTGCC G^G[CT][GA]CC
388 GGTACC
BanII 536 GGGCTC G[GA]GC[CT]^C
1210 GGGCTC
BcnI 262 CCGGG CC^[GC]GG
482 CCGGG
BsiHKAI 528 GAGCAC G[AT]GC[AT]^C
608 GTGCTC
BsmAI 92 GAGAC GTCTC(1/5)
157 GAGAC
BsoBI 11 CTCGGG C^[CT]CG[GA]G
250 CTCGAG
BstF5I 636 CATCC GGATG(2/0)
1220 CATCC
BstYI 289 GGATCC [GA]^GATC[CT]
472 GGATCT
FokI 636 CATCC GGATG(9/13)
1220 CATCC
MaeII 729 ACGT A^CGT
856 ACGT
MflI 289 GGATCC [GA]^GATC[CT]
472 GGATCT
MwoI 299 GCGGAGGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
431 GCCCAGGAGGC
NciI 262 CCGGG CC^[GC]GG
482 CCGGG
PpuMI 406 AGGACCC [GA]G^G[AT]CC[CT]
626 AGGTCCT
SmlI 250 CTCGAG C^T[CT][GA]AG
819 CTTGAG
TfiI 217 GATTC G^A[AT]TC
1152 GATTC
Tsp45I 682 GTCAC ^GT[GC]AC
949 GTGAC
XhoII 289 GGATCC [GA]^GATC[CT]
472 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BfaI BmyI BpmI BsaJI BsiYI
BslI BsmFI Bsp1286I BssKI BstNI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I Fnu4HI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI StyI Tru9I TseI
Tsp509I XcmI