Restriction map for
pF1KB8832
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AlwI AocI
ApoI AscI AseI AsnI Asp718I
AspI AvrII BamHI BbsI BbvCI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BsaWI BseAI BseRI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DpnII DraI DraII DraIII
DrdI DsaI Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HpaI KpnI
KspI MamI MboI MfeI MflI
MluI MroI MseI MunI NcoI
NdeI NdeII NheI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI Sau3AI SbfI ScaI
SfiI SfuI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TaqI TfiI Tru9I Tsp509I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AfaI 762 GTAC GT^AC
AflIII 554 ACGTGT A^C[GA][CT]GT
AhdI 575 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 361 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1244 GGGCCC GGGCC^C
AspEI 575 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 972 GGACC G^G[AT]CC
AviII 1189 TGCGCA TGC^GCA
BalI 195 TGGCCA TGG^CCA
BfaI 133 CTAG C^TAG
BpmI 709 CTGGAG CTGGAG(16/14)
BsiWI 761 CGTACG C^GTACG
BsmAI 583 GTCTC GTCTC(1/5)
BsmI 136 GAATGC GAATGC(1/-1)
BspMI 650 ACCTGC ACCTGC(4/8)
BssSI 42 CTCGTG CACGAG(-5/-1)
BstXI 594 CCACCAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eam1105I 575 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 427 CTCTTC CTCTTC(1/4)
EcoT14I 452 CCTTGG C^C[AT][AT]GG
FspI 1189 TGCGCA TGC^GCA
HinfI 537 GAGTC G^A[ACGT]TC
Ksp632I 427 CTCTTC CTCTTC(1/4)
MaeI 133 CTAG C^TAG
MboII 428 TCTTC GAAGA(8/7)
MluNI 195 TGGCCA TGG^CCA
MscI 195 TGGCCA TGG^CCA
NotI 947 GCGGCCGC GC^GGCCGC
PleI 537 GAGTC GAGTC(4/5)
RsaI 762 GTAC GT^AC
SapI 426 GCTCTTC GCTCTTC(1/4)
SexAI 890 ACCTGGT A^CC[AT]GGT
SphI 189 GCATGC GCATG^C
StuI 1121 AGGCCT AGG^CCT
StyI 452 CCTTGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Alw44I 152 GTGCAC G^TGCAC
898 GTGCAC
Aor51HI 146 AGCGCT AGC^GCT
881 AGCGCT
ApaLI 152 GTGCAC G^TGCAC
898 GTGCAC
Asp700I 672 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
924 GAAGCCCTTC
BanI 353 GGCGCC G^G[CT][GA]CC
523 GGCGCC
BbeI 353 GGCGCC GGCGC^C
523 GGCGCC
BbrPI 553 CACGTG CAC^GTG
1044 CACGTG
BcgI 866 GCAAGGGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1298 GCACTGCCCTCG
BsaAI 553 CACGTG [CT]AC^GT[GA]
1044 CACGTG
BsmFI 457 GGGAC GGGAC(10/14)
558 GTCCC
BsrI 597 CCAGT ACTGG(1/-1)
1284 CCAGT
BstAPI 154 GCACGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
215 GCAGTGAGTGC
DdeI 78 CTCAG C^T[ACGT]AG
585 CTCAG
Eco47III 146 AGCGCT AGC^GCT
881 AGCGCT
HindIII 231 AAGCTT A^AGCTT
1141 AAGCTT
KasI 353 GGCGCC G^GCGCC
523 GGCGCC
NarI 353 GGCGCC GG^CGCC
523 GGCGCC
PmaCI 553 CACGTG CAC^GTG
1044 CACGTG
PmlI 553 CACGTG CAC^GTG
1044 CACGTG
PstI 213 CTGCAG CTGCA^G
652 CTGCAG
SacI 101 GAGCTC GAGCT^C
630 GAGCTC
SfoI 353 GGCGCC GGC^GCC
523 GGCGCC
SgrAI 349 CACCGGCG C[GA]^CCGG[CT]G
985 CACCGGCG
XmnI 672 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
924 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
AvaI BanII BbvI BcnI BglI
BmyI BsaHI BsaJI BsgI BsiEI
BsiHKAI BsiYI BslI BsoBI Bsp1286I
BsrBI BsrFI BssHII BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I EaeI EagI EclXI Eco52I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI MaeII
MaeIII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NgoMIV NlaIII NlaIV NspI Sau96I
ScrFI SfaNI SfcI TseI Tsp45I
TspRI