Restriction map for
pF1KB8831
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# NO CUT ENZYMES ( 155 enzymes) #
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Acc65I AccI AccII AccIII AciI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BcnI BfrI BglI
BlnI BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Cac8I CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoT22I FauI FseI FspI
HaeII HapII HgaI HhaI HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MamI MluI
MroI MspA1I MspI MvnI MwoI
NaeI NarI NciI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI PvuI PvuII
RsaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 297 GACGTC GACGT^C
AclI 1058 AACGTT AA^CGTT
AcyI 297 GACGTC G[GA]^CG[CT]C
AlwI 312 GGATC GGATC(4/5)
AocI 799 CCTCAGG CC^T[ACGT]AGG
AspHI 10 GAGCTC G[AT]GC[AT]^C
AvaII 996 GGTCC G^G[AT]CC
BalI 604 TGGCCA TGG^CCA
BclI 761 TGATCA T^GATCA
BglII 1084 AGATCT A^GATCT
BlpI 573 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 573 GCTGAGC GC^T[ACGT]AGC
BsaHI 297 GACGTC G[GA]^CG[CT]C
BsiHKAI 10 GAGCTC G[AT]GC[AT]^C
BsrDI 569 CATTGC GCAATG(2/0)
BssKI 991 CCAGG ^CC[ACGT]GG
BstNI 991 CCAGG CC^[AT]GG
BstYI 1084 AGATCT [GA]^GATC[CT]
Bsu36I 799 CCTCAGG CC^T[ACGT]AGG
CelII 573 GCTGAGC GC^T[ACGT]AGC
DraII 995 GGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 462 CCATGG C^C[GA][CT]GG
EaeI 604 TGGCCA [CT]^GGCC[GA]
Eco81I 799 CCTCAGG CC^T[ACGT]AGG
EcoO109I 995 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 69 GAATTC G^AATTC
EcoRII 991 CCAGG ^CC[AT]GG
EcoRV 259 GATATC GAT^ATC
EcoT14I 462 CCATGG C^C[AT][AT]GG
FbaI 761 TGATCA T^GATCA
Hin1I 297 GACGTC G[GA]^CG[CT]C
MfeI 401 CAATTG C^AATTG
MflI 1084 AGATCT [GA]^GATC[CT]
MluNI 604 TGGCCA TGG^CCA
MscI 604 TGGCCA TGG^CCA
MslI 691 CATACAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 401 CAATTG C^AATTG
MvaI 991 CCAGG CC^[AT]GG
NcoI 462 CCATGG C^CATGG
NspI 880 GCATGT [GA]CATG^[CT]
PleI 598 GAGTC GAGTC(4/5)
PpuMI 995 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 297 GACGTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1058 AACGTT AA^CGTT
PstI 702 CTGCAG CTGCA^G
SacI 10 GAGCTC GAGCT^C
ScrFI 991 CCAGG CC^[ACGT]GG
SfaNI 1143 GATGC GCATC(5/9)
SmlI 911 CTCAAG C^T[CT][GA]AG
StyI 462 CCATGG C^C[AT][AT]GG
XhoII 1084 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 69 GAATTC [GA]^AATT[CT]
1029 AAATTC
ApoI 69 GAATTC [GA]^AATT[CT]
1029 AAATTC
BanII 10 GAGCTC G[GA]GC[CT]^C
136 GGGCTC
BfaI 496 CTAG C^TAG
649 CTAG
BmyI 10 GAGCTC G[AGT]GC[ACT]^C
136 GGGCTC
BpmI 896 CTCCAG CTGGAG(16/14)
924 CTCCAG
BsaJI 462 CCATGG C^C[ACGT][ACGT]GG
991 CCAGGG
BsiYI 81 CCCAGTCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
407 CCATTGTCAGG
BslI 81 CCCAGTCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
407 CCATTGTCAGG
BsmFI 997 GTCCC GGGAC(10/14)
1311 GTCCC
Bsp1286I 10 GAGCTC G[AGT]GC[ACT]^C
136 GGGCTC
BspHI 197 TCATGA T^CATGA
1041 TCATGA
BspMI 891 ACCTGC ACCTGC(4/8)
581 GCAGGT
Cfr13I 796 GGGCC G^G[ACGT]CC
996 GGTCC
EarI 773 CTCTTC CTCTTC(1/4)
969 CTCTTC
HphI 804 GGTGA GGTGA(8/7)
283 TCACC
Ksp632I 773 CTCTTC CTCTTC(1/4)
969 CTCTTC
MaeI 496 CTAG C^TAG
649 CTAG
MaeII 298 ACGT A^CGT
1059 ACGT
MnlI 234 CCTC CCTC(7/6)
799 CCTC
NdeI 421 CATATG CA^TATG
1330 CATATG
NlaIV 995 GGGTCC GG[ACGT]^[ACGT]CC
996 GGTCCC
RcaI 197 TCATGA T^CATGA
1041 TCATGA
Sau96I 796 GGGCC G^G[ACGT]CC
996 GGTCC
SfcI 702 CTGCAG C^T[GA][CT]AG
1101 CTACAG
TaqI 14 TCGA T^CGA
1089 TCGA
TfiI 174 GAATC G^A[AT]TC
613 GATTC
TthHB8I 14 TCGA T^CGA
1089 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AluI BbsI BbvI BpuAI BsrI
BstF5I DdeI DpnII Fnu4HI FokI
HaeIII HinfI ItaI MaeIII MboI
MboII MseI NdeII NlaIII Sau3AI
Tru9I TseI Tsp509I TspRI