Restriction map for
pF1KB8823
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BsaBI BsaI BsaWI BseAI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
ClaI CpoI DraI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HinfI HpaI KpnI KspI
MaeI MamI MfeI MluI MroI
MseI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SapI SbfI ScaI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 445 GTCGAC GT^[AC][GT]AC
AcyI 761 GGCGCC G[GA]^CG[CT]C
AfaI 630 GTAC GT^AC
AflIII 407 ACATGT A^C[GA][CT]GT
AlwI 75 GATCC GGATC(4/5)
AlwNI 633 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
BbeI 761 GGCGCC GGCGC^C
BbrPI 130 CACGTG CAC^GTG
BbsI 706 GTCTTC GAAGAC(2/6)
BglI 784 GCCTACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 138 GCTCAGC GC^T[ACGT]AGC
BpmI 331 CTGGAG CTGGAG(16/14)
Bpu1102I 138 GCTCAGC GC^T[ACGT]AGC
BpuAI 706 GTCTTC GAAGAC(2/6)
BsaHI 761 GGCGCC G[GA]^CG[CT]C
BseRI 1050 CTCCTC GAGGAG(10/8)
BsiEI 447 CGACCG CG[GA][CT]^CG
BsmAI 312 GTCTC GTCTC(1/5)
BsmFI 853 GGGAC GGGAC(10/14)
BspLU11I 407 ACATGT A^CATGT
BspMI 887 ACCTGC ACCTGC(4/8)
BstAPI 498 GCACCGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 42 GGTCACC G^GT[ACGT]ACC
BstPI 42 GGTCACC G^GT[ACGT]ACC
BstYI 74 AGATCC [GA]^GATC[CT]
CelII 138 GCTCAGC GC^T[ACGT]AGC
DpnII 75 GATC ^GATC
DraIII 499 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 448 GACCGCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1014 CCTTCCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 42 GGTCACC G^GT[ACGT]ACC
HgaI 227 GCGTC GACGC(5/10)
Hin1I 761 GGCGCC G[GA]^CG[CT]C
KasI 761 GGCGCC G^GCGCC
MboI 75 GATC ^GATC
MflI 74 AGATCC [GA]^GATC[CT]
NarI 761 GGCGCC GG^CGCC
NdeII 75 GATC ^GATC
PflMI 576 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 130 CACGTG CAC^GTG
PmlI 130 CACGTG CAC^GTG
PstI 1112 CTGCAG CTGCA^G
RsaI 630 GTAC GT^AC
SacI 136 GAGCTC GAGCT^C
SalI 445 GTCGAC G^TCGAC
Sau3AI 75 GATC ^GATC
SexAI 878 ACCTGGT A^CC[AT]GGT
SfoI 761 GGCGCC GGC^GCC
TaqI 446 TCGA T^CGA
TthHB8I 446 TCGA T^CGA
Van91I 576 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 74 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AocI 559 CCTGAGG CC^T[ACGT]AGG
1018 CCTAAGG
ApaI 588 GGGCCC GGGCC^C
641 GGGCCC
AspHI 136 GAGCTC G[AT]GC[AT]^C
147 GAGCAC
BalI 182 TGGCCA TGG^CCA
284 TGGCCA
BanI 615 GGCACC G^G[CT][GA]CC
761 GGCGCC
BcnI 249 CCGGG CC^[GC]GG
981 CCGGG
BsaAI 130 CACGTG [CT]AC^GT[GA]
787 TACGTG
BsgI 931 CTGCAC GTGCAG(16/14)
521 GTGCAG
BsiHKAI 136 GAGCTC G[AT]GC[AT]^C
147 GAGCAC
BsmI 996 GAATGC GAATGC(1/-1)
1074 GCATTC
Bsu36I 559 CCTGAGG CC^T[ACGT]AGG
1018 CCTAAGG
Eco81I 559 CCTGAGG CC^T[ACGT]AGG
1018 CCTAAGG
HaeII 469 AGCGCC [GA]GCGC^[CT]
761 GGCGCC
MluNI 182 TGGCCA TGG^CCA
284 TGGCCA
MscI 182 TGGCCA TGG^CCA
284 TGGCCA
MslI 45 CACCGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
186 CATGTTTGTG
NciI 249 CCGGG CC^[GC]GG
981 CCGGG
NspI 407 ACATGT [GA]CATG^[CT]
1042 ACATGC
PpuMI 533 GGGTCCT [GA]G^G[AT]CC[CT]
853 GGGACCC
PvuII 15 CAGCTG CAG^CTG
577 CAGCTG
SfcI 1001 CTGTAG C^T[GA][CT]AG
1112 CTGCAG
XcmI 287 CCATCGCGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
662 CCACCACCATCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AluI AvaII BanII
BbvI BmyI BsaJI BsiYI BslI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HpaII HphI
ItaI Ksp632I MaeII MaeIII MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NgoMIV NlaIII NlaIV
Sau96I ScrFI SfaNI StyI TseI
Tsp45I TspRI