Restriction map for
pF1KB8820
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII BamHI BanII BbeI BbrPI
BcgI BciVI BclI BfrI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI TaqI Tsp509I Tth111I TthHB8I
XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AlwNI 961 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 360 GAGCAC G[AT]GC[AT]^C
AvaI 470 CCCGGG C^[CT]CG[GA]G
AvrII 648 CCTAGG C^CTAGG
BalI 263 TGGCCA TGG^CCA
BglI 225 GCCTCGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 245 AGATCT A^GATCT
BlnI 648 CCTAGG C^CTAGG
BlpI 445 GCTCAGC GC^T[ACGT]AGC
BmyI 360 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 445 GCTCAGC GC^T[ACGT]AGC
BsaAI 220 TACGTG [CT]AC^GT[GA]
BsiHKAI 360 GAGCAC G[AT]GC[AT]^C
BsmAI 523 GTCTC GTCTC(1/5)
BsmBI 522 CGTCTC CGTCTC(1/5)
BsoBI 470 CCCGGG C^[CT]CG[GA]G
Bsp1286I 360 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 546 TGTACA T^GTACA
BspMI 830 ACCTGC ACCTGC(4/8)
BsrGI 546 TGTACA T^GTACA
BstYI 245 AGATCT [GA]^GATC[CT]
CelII 445 GCTCAGC GC^T[ACGT]AGC
DraI 744 TTTAAA TTT^AAA
DraII 466 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 314 CCGTGG C^C[GA][CT]GG
EarI 622 CTCTTC CTCTTC(1/4)
EcoNI 452 CCTACCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 466 AGGACCC [GA]G^G[ACGT]CC[CT]
Ksp632I 622 CTCTTC CTCTTC(1/4)
MaeII 221 ACGT A^CGT
MflI 245 AGATCT [GA]^GATC[CT]
MluNI 263 TGGCCA TGG^CCA
MscI 263 TGGCCA TGG^CCA
MslI 309 CATCTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI 497 CCATAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 466 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 1035 CAGCTG CAG^CTG
SapI 621 GCTCTTC GCTCTTC(1/4)
SmaI 470 CCCGGG CCC^GGG
SspBI 546 TGTACA T^GTACA
StuI 1022 AGGCCT AGG^CCT
Van91I 497 CCATAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 867 TCTAGA T^CTAGA
XcmI 527 CCATCCCTGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 245 AGATCT [GA]^GATC[CT]
XmaCI 470 CCCGGG C^CCGGG
XmaI 470 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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Asp700I 618 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
969 GAAGGCTTTC
BanI 998 GGCACC G^G[CT][GA]CC
1005 GGCACC
BfaI 649 CTAG C^TAG
868 CTAG
BsmFI 607 GGGAC GGGAC(10/14)
513 GTCCC
HapII 471 CCGG C^CGG
738 CCGG
HinfI 46 GATTC G^A[ACGT]TC
258 GAATC
HpaII 471 CCGG C^CGG
738 CCGG
MaeI 649 CTAG C^TAG
868 CTAG
MseI 377 TTAA T^TAA
745 TTAA
MspA1I 183 CAGCGG C[AC]G^C[GT]G
1035 CAGCTG
MspI 471 CCGG C^CGG
738 CCGG
PstI 105 CTGCAG CTGCA^G
755 CTGCAG
TfiI 46 GATTC G^A[AT]TC
258 GAATC
Tru9I 377 TTAA T^TAA
745 TTAA
Tsp45I 289 GTGAC ^GT[GC]AC
947 GTCAC
XmnI 618 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
969 GAAGGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI AluI AvaII BbsI
BbvCI BbvI BcnI BpmI BpuAI
BsaJI BsiYI BslI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EaeI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HphI ItaI
MaeIII MboI MboII MnlI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TseI TspRI