Restriction map for
pF1KB8807
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII AccI AccII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AvaI AviII AvrII BbeI BbrPI
BbsI BcgI BclI BfrI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsgI BsiEI BsiWI BsiYI
BslI BsmBI BsmFI BsmI BsoBI
BspDI BspHI BspLU11I BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KspI
MamI MfeI MluI MseI MunI
MvnI NaeI NarI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tru9I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccIII 923 TCCGGA T^CCGGA
AcsI 502 GAATTT [GA]^AATT[CT]
ApoI 502 GAATTT [GA]^AATT[CT]
AspI 838 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 269 GGATCC G^GATCC
BanII 428 GAGCCC G[GA]GC[CT]^C
BbvCI 678 GCTGAGG CCTCAGC(-5/-2)
BciVI 567 GGATAC GTATCC(6/5)
BglI 143 GCCTCTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 359 AGATCT A^GATCT
BpmI 582 CTGGAG CTGGAG(16/14)
BsaI 25 GAGACC GGTCTC(1/5)
BsaWI 923 TCCGGA [AT]^CCGG[AT]
BseAI 923 TCCGGA T^CCGGA
BseRI 322 CTCCTC GAGGAG(10/8)
Bsp1407I 350 TGTACA T^GTACA
BspEI 923 TCCGGA T^CCGGA
BsrBI 820 GAGCGG CCGCTC(-3/-3)
BsrDI 152 GCAATG GCAATG(2/0)
BsrGI 350 TGTACA T^GTACA
CfoI 936 GCGC GCG^C
DraII 473 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 306 CCATGG C^C[GA][CT]GG
EcoO109I 473 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 306 CCATGG C^C[AT][AT]GG
FauI 114 CCCGC CCCGC(4/6)
HhaI 936 GCGC GCG^C
HinP1I 936 GCGC G^CGC
MaeII 56 ACGT A^CGT
MroI 923 TCCGGA T^CCGGA
MspA1I 115 CCGCTG C[AC]G^C[GT]G
NcoI 306 CCATGG C^CATGG
NheI 486 GCTAGC G^CTAGC
NspI 311 GCATGT [GA]CATG^[CT]
PflFI 838 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 540 GACTC GAGTC(4/5)
SexAI 839 ACCTGGT A^CC[AT]GGT
SspBI 350 TGTACA T^GTACA
StyI 306 CCATGG C^C[AT][AT]GG
TfiI 662 GAATC G^A[AT]TC
Tth111I 838 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 86 CCAGAGCACTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Acc65I 34 GGTACC G^GTACC
392 GGTACC
Asp718I 34 GGTACC G^GTACC
392 GGTACC
AspHI 89 GAGCAC G[AT]GC[AT]^C
551 GTGCTC
AvaII 237 GGACC G^G[AT]CC
837 GGACC
BcnI 431 CCCGG CC^[GC]GG
891 CCCGG
BsiHKAI 89 GAGCAC G[AT]GC[AT]^C
551 GTGCTC
BstYI 269 GGATCC [GA]^GATC[CT]
359 AGATCT
HinfI 540 GACTC G^A[ACGT]TC
662 GAATC
KpnI 34 GGTACC GGTAC^C
392 GGTACC
MflI 269 GGATCC [GA]^GATC[CT]
359 AGATCT
MslI 732 CATGGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
846 CATGCTGGTG
NciI 431 CCCGG CC^[GC]GG
891 CCCGG
SapI 243 GCTCTTC GCTCTTC(1/4)
493 GCTCTTC
SfaNI 461 GCATC GCATC(5/9)
831 GCATC
TaqI 386 TCGA T^CGA
426 TCGA
Tsp509I 198 AATT ^AATT
503 AATT
TthHB8I 386 TCGA T^CGA
426 TCGA
XhoII 269 GGATCC [GA]^GATC[CT]
359 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AlwI BalI
BanI BbvI BfaI BmyI BsaJI
BsmAI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EaeI EarI EcoRII Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MluNI MnlI MscI MspI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI TseI
Tsp45I TspRI