Restriction map for
pF1KB8803
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspI AvrII
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraII DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HaeII HincII HindII
HpaI KasI KpnI KspI MluI
MroI MslI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcsI 173 AAATTC [GA]^AATT[CT]
AcyI 6 GACGCC G[GA]^CG[CT]C
AhdI 545 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1024 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 173 AAATTC [GA]^AATT[CT]
Asp700I 655 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 545 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 472 GAGCTC G[AT]GC[AT]^C
AvaI 116 CCCGAG C^[CT]CG[GA]G
AviII 1031 TGCGCA TGC^GCA
BglI 766 GCCAATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 321 GATCGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 6 GACGCC G[GA]^CG[CT]C
BsiHKAI 472 GAGCTC G[AT]GC[AT]^C
BsmBI 4 GAGACG CGTCTC(1/5)
BsoBI 116 CCCGAG C^[CT]CG[GA]G
BsrBI 838 GAGCGG CCGCTC(-3/-3)
BstXI 412 CCAGAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1032 GCGC GCG^C
DraI 855 TTTAAA TTT^AAA
Eam1105I 545 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 707 CTCTTC CTCTTC(1/4)
EcoNI 924 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 840 GCGGG CCCGC(4/6)
FspI 1031 TGCGCA TGC^GCA
HgaI 6 GACGC GACGC(5/10)
HhaI 1032 GCGC GCG^C
Hin1I 6 GACGCC G[GA]^CG[CT]C
HinP1I 1032 GCGC G^CGC
HindIII 275 AAGCTT A^AGCTT
HphI 508 GGTGA GGTGA(8/7)
Ksp632I 707 CTCTTC CTCTTC(1/4)
MaeII 639 ACGT A^CGT
MaeIII 824 GTGAC ^GT[ACGT]AC
MamI 321 GATCGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 23 CAATTG C^AATTG
MseI 856 TTAA T^TAA
MspA1I 526 CAGCTG C[AC]G^C[GT]G
MunI 23 CAATTG C^AATTG
PflMI 336 CCATGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 526 CAGCTG CAG^CTG
SacI 472 GAGCTC GAGCT^C
SapI 706 GCTCTTC GCTCTTC(1/4)
SexAI 735 ACCTGGT A^CC[AT]GGT
StuI 860 AGGCCT AGG^CCT
TaqI 663 TCGA T^CGA
TfiI 88 GATTC G^A[AT]TC
Tru9I 856 TTAA T^TAA
Tsp45I 824 GTGAC ^GT[GC]AC
TspRI 563 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 663 TCGA T^CGA
Van91I 336 CCATGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 177 TCTAGA T^CTAGA
XcmI 885 CCAGGCTGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 655 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
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AfaI 291 GTAC GT^AC
376 GTAC
AvaII 544 GGACC G^G[AT]CC
739 GGTCC
BanII 472 GAGCTC G[GA]GC[CT]^C
704 GGGCTC
BpmI 743 CTCCAG CTGGAG(16/14)
601 CTGGAG
BsaI 119 GAGACC GGTCTC(1/5)
265 GAGACC
BstF5I 569 CATCC GGATG(2/0)
1008 GGATG
BstYI 159 AGATCC [GA]^GATC[CT]
945 AGATCC
FokI 569 CATCC GGATG(9/13)
1008 GGATG
MflI 159 AGATCC [GA]^GATC[CT]
945 AGATCC
NcoI 336 CCATGG C^CATGG
448 CCATGG
RsaI 291 GTAC GT^AC
376 GTAC
Tsp509I 24 AATT ^AATT
174 AATT
XhoII 159 AGATCC [GA]^GATC[CT]
945 AGATCC
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI AlwI BalI BbvI
BcnI BfaI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstNI Cac8I Cfr13I DdeI DpnII
DsaI EaeI EcoRII EcoT14I Fnu4HI
HaeIII HapII HinfI HpaII ItaI
MaeI MboI MboII MluNI MnlI
MscI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TseI