Restriction map for
pF1KB8797
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BpuAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BssSI Bst1107I
BstBI BstEII BstF5I BstPI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FokI FseI
FspI HindIII HinfI HpaI HphI
KasI KpnI MamI MfeI MluI
MroI MseI MunI NarI NcoI
NdeI NheI NotI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StyI
SwaI TfiI Tru9I Tsp509I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 31 enzymes) #
###################################
Asp700I 831 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 261 CCCGAG C^[CT]CG[GA]G
BanI 591 GGTGCC G^G[CT][GA]CC
BfaI 526 CTAG C^TAG
BsoBI 261 CCCGAG C^[CT]CG[GA]G
Bsp1407I 422 TGTACA T^GTACA
BsrGI 422 TGTACA T^GTACA
BstXI 842 CCAAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 317 AGATCC [GA]^GATC[CT]
DraII 59 AGGACCT [GA]G^G[ACGT]CC[CT]
EarI 490 CTCTTC CTCTTC(1/4)
EcoO109I 59 AGGACCT [GA]G^G[ACGT]CC[CT]
HincII 199 GTCAAC GT[CT]^[GA]AC
HindII 199 GTCAAC GT[CT]^[GA]AC
Ksp632I 490 CTCTTC CTCTTC(1/4)
KspI 400 CCGCGG CCGC^GG
MaeI 526 CTAG C^TAG
MboII 491 TCTTC GAAGA(8/7)
MflI 317 AGATCC [GA]^GATC[CT]
NaeI 674 GCCGGC GCC^GGC
NgoMIV 674 GCCGGC G^CCGGC
NruI 781 TCGCGA TCG^CGA
PpuMI 59 AGGACCT [GA]G^G[AT]CC[CT]
SacI 264 GAGCTC GAGCT^C
SacII 400 CCGCGG CCGC^GG
SapI 489 GCTCTTC GCTCTTC(1/4)
SfaNI 540 GATGC GCATC(5/9)
SspBI 422 TGTACA T^GTACA
StuI 216 AGGCCT AGG^CCT
XhoII 317 AGATCC [GA]^GATC[CT]
XmnI 831 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 562 GACGCC G[GA]^CG[CT]C
738 GACGCC
AflIII 188 ACGTGT A^C[GA][CT]GT
437 ACGTGT
AlwI 318 GATCC GGATC(4/5)
753 GATCC
BbrPI 304 CACGTG CAC^GTG
436 CACGTG
BlpI 225 GCTCAGC GC^T[ACGT]AGC
366 GCTGAGC
BpmI 858 CTCCAG CTGGAG(16/14)
679 CTGGAG
Bpu1102I 225 GCTCAGC GC^T[ACGT]AGC
366 GCTGAGC
BsaHI 562 GACGCC G[GA]^CG[CT]C
738 GACGCC
BseRI 480 CTCCTC GAGGAG(10/8)
40 GAGGAG
BsgI 820 CTGCAC GTGCAG(16/14)
982 GTGCAG
BsmFI 665 GGGAC GGGAC(10/14)
950 GGGAC
BsrFI 674 GCCGGC [GA]^CCGG[CT]
953 ACCGGC
BssHII 461 GCGCGC G^CGCGC
521 GCGCGC
BstAPI 550 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
984 GCAGCAGGTGC
CelII 225 GCTCAGC GC^T[ACGT]AGC
366 GCTGAGC
Cfr10I 674 GCCGGC [GA]^CCGG[CT]
953 ACCGGC
DdeI 226 CTCAG C^T[ACGT]AG
367 CTGAG
DpnII 318 GATC ^GATC
753 GATC
DraIII 711 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
823 CACTTTGTG
DsaI 400 CCGCGG C^C[GA][CT]GG
794 CCGTGG
HaeII 229 AGCGCC [GA]GCGC^[CT]
276 GGCGCT
Hin1I 562 GACGCC G[GA]^CG[CT]C
738 GACGCC
MaeIII 78 GTCAC ^GT[ACGT]AC
871 GTGAC
MboI 318 GATC ^GATC
753 GATC
NdeII 318 GATC ^GATC
753 GATC
PmaCI 304 CACGTG CAC^GTG
436 CACGTG
PmlI 304 CACGTG CAC^GTG
436 CACGTG
PvuII 12 CAGCTG CAG^CTG
67 CAGCTG
Sau3AI 318 GATC ^GATC
753 GATC
TaqI 374 TCGA T^CGA
386 TCGA
Tsp45I 78 GTCAC ^GT[GC]AC
871 GTGAC
TthHB8I 374 TCGA T^CGA
386 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
AlwNI ApaLI AspHI AvaII BalI
BanII BbvI BcnI BmyI BsaAI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BspMI BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr13I EaeI EcoRII
FauI Fnu4HI HaeIII HapII HgaI
HhaI HinP1I HpaII ItaI MaeII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV PstI RsaI Sau96I
ScrFI SfcI TseI TspRI