Restriction map for
pF1KB8794
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BcnI BfrI BglII BlnI
BpmI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiWI BsmBI
BsmFI BsoBI BspDI BspEI BspHI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FseI FspI HaeII HapII HgaI
Hin1I HindIII HinfI HpaI HpaII
KasI KpnI KspI MamI MfeI
MluI MroI MseI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StyI
SwaI TfiI Tru9I Tsp509I Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AfaI 414 GTAC GT^AC
AflIII 547 ACATGT A^C[GA][CT]GT
AocI 118 CCTAAGG CC^T[ACGT]AGG
AspHI 136 GTGCTC G[AT]GC[AT]^C
BbsI 298 GTCTTC GAAGAC(2/6)
BclI 872 TGATCA T^GATCA
BfaI 178 CTAG C^TAG
BlpI 311 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 311 GCTCAGC GC^T[ACGT]AGC
BpuAI 298 GTCTTC GAAGAC(2/6)
BsiEI 396 CGACCG CG[GA][CT]^CG
BsiHKAI 136 GTGCTC G[AT]GC[AT]^C
BsmAI 439 GAGAC GTCTC(1/5)
BsmI 171 GAATGC GAATGC(1/-1)
Bsp1407I 413 TGTACA T^GTACA
BspLU11I 547 ACATGT A^CATGT
BsrBI 972 CCGCTC CCGCTC(-3/-3)
BsrGI 413 TGTACA T^GTACA
BstXI 188 CCATCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 956 AGATCC [GA]^GATC[CT]
Bsu36I 118 CCTAAGG CC^T[ACGT]AGG
CelII 311 GCTCAGC GC^T[ACGT]AGC
CfoI 175 GCGC GCG^C
DraII 84 GGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 712 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 948 CTCTTC CTCTTC(1/4)
Eco81I 118 CCTAAGG CC^T[ACGT]AGG
EcoO109I 84 GGGGCCC [GA]G^G[ACGT]CC[CT]
FbaI 872 TGATCA T^GATCA
HhaI 175 GCGC GCG^C
HinP1I 175 GCGC G^CGC
HincII 394 GTCGAC GT[CT]^[GA]AC
HindII 394 GTCGAC GT[CT]^[GA]AC
Ksp632I 948 CTCTTC CTCTTC(1/4)
MaeI 178 CTAG C^TAG
MaeII 53 ACGT A^CGT
MaeIII 448 GTGAC ^GT[ACGT]AC
MflI 956 AGATCC [GA]^GATC[CT]
NspI 547 ACATGT [GA]CATG^[CT]
PflMI 711 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 628 CTGCAG CTGCA^G
PvuII 38 CAGCTG CAG^CTG
RsaI 414 GTAC GT^AC
SalI 394 GTCGAC G^TCGAC
SfcI 628 CTGCAG C^T[GA][CT]AG
SspBI 413 TGTACA T^GTACA
TaqI 395 TCGA T^CGA
Tsp45I 448 GTGAC ^GT[GC]AC
TthHB8I 395 TCGA T^CGA
Van91I 711 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 956 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 11 enzymes) #
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AluI 39 AGCT AG^CT
632 AGCT
AlwI 957 GATCC GGATC(4/5)
968 GATCC
ApaI 72 GGGCCC GGGCC^C
85 GGGCCC
BanII 72 GGGCCC G[GA]GC[CT]^C
85 GGGCCC
BglI 530 GCCTGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
739 GCCACACTGGC
BspMI 257 ACCTGC ACCTGC(4/8)
695 ACCTGC
DsaI 248 CCGTGG C^C[GA][CT]GG
749 CCGTGG
FauI 725 CCCGC CCCGC(4/6)
971 CCCGC
HphI 808 GGTGA GGTGA(8/7)
425 TCACC
MslI 153 CACCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
189 CATCATCGTG
StuI 122 AGGCCT AGG^CCT
267 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccI AciI AvaII BalI BanI
BbvI BmyI BsaJI BsgI BsiYI
BslI Bsp1286I BsrDI BsrI BssKI
BstAPI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EaeI EcoRII Fnu4HI
FokI HaeIII ItaI MboI MboII
MluNI MnlI MscI MspA1I MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI TseI TspRI
XcmI