Restriction map for
pF1KB8793
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvaI AvrII BalI
BbeI BbrPI BcgI BciVI BclI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI StyI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 196 GTCTAC GT^[AC][GT]AC
Aor51HI 377 AGCGCT AGC^GCT
ApaI 461 GGGCCC GGGCC^C
Asp700I 183 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 599 GGTCC G^G[AT]CC
AviII 176 TGCGCA TGC^GCA
BanI 958 GGCACC G^G[CT][GA]CC
BbsI 100 GTCTTC GAAGAC(2/6)
BfaI 305 CTAG C^TAG
BpuAI 100 GTCTTC GAAGAC(2/6)
BsaI 85 GAGACC GGTCTC(1/5)
BseRI 697 CTCCTC GAGGAG(10/8)
BsiEI 440 CGGTCG CG[GA][CT]^CG
BsmAI 85 GAGAC GTCTC(1/5)
BsmFI 468 GTCCC GGGAC(10/14)
BsmI 719 GCATTC GAATGC(1/-1)
DraII 461 GGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 212 TGGCCG [CT]^GGCC[GA]
EarI 229 CTCTTC CTCTTC(1/4)
Eco47III 377 AGCGCT AGC^GCT
EcoO109I 461 GGGCCCT [GA]G^G[ACGT]CC[CT]
FspI 176 TGCGCA TGC^GCA
HaeII 377 AGCGCT [GA]GCGC^[CT]
Ksp632I 229 CTCTTC CTCTTC(1/4)
MaeI 305 CTAG C^TAG
MaeII 200 ACGT A^CGT
MseI 809 TTAA T^TAA
MspA1I 437 CAGCGG C[AC]G^C[GT]G
PleI 526 GACTC GAGTC(4/5)
PshAI 428 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmlI 886 CTCAAG C^T[CT][GA]AG
SspI 735 AATATT AAT^ATT
StuI 345 AGGCCT AGG^CCT
TaqI 973 TCGA T^CGA
TfiI 754 GATTC G^A[AT]TC
Tru9I 809 TTAA T^TAA
Tsp45I 524 GTGAC ^GT[GC]AC
TthHB8I 973 TCGA T^CGA
XmnI 183 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AluI 307 AGCT AG^CT
890 AGCT
AspHI 151 GTGCTC G[AT]GC[AT]^C
360 GAGCAC
BamHI 3 GGATCC G^GATCC
623 GGATCC
BbvCI 204 CCTCAGC CCTCAGC(-5/-2)
922 GCTGAGG
BlpI 357 GCTGAGC GC^T[ACGT]AGC
477 GCTGAGC
BpmI 636 CTCCAG CTGGAG(16/14)
898 CTCCAG
Bpu1102I 357 GCTGAGC GC^T[ACGT]AGC
477 GCTGAGC
BsiHKAI 151 GTGCTC G[AT]GC[AT]^C
360 GAGCAC
BsrDI 117 CATTGC GCAATG(2/0)
918 CATTGC
BsrI 602 CCAGT ACTGG(1/-1)
784 CCAGT
CelII 357 GCTGAGC GC^T[ACGT]AGC
477 GCTGAGC
CfoI 177 GCGC GCG^C
378 GCGC
HhaI 177 GCGC GCG^C
378 GCGC
HinP1I 177 GCGC G^CGC
378 GCGC
HinfI 526 GACTC G^A[ACGT]TC
754 GATTC
HphI 523 GGTGA GGTGA(8/7)
314 TCACC
MaeIII 272 GTAAC ^GT[ACGT]AC
524 GTGAC
MslI 315 CACCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
318 CACTGTGATG
NspI 173 GCATGC [GA]CATG^[CT]
353 GCATGC
SfaNI 944 GATGC GCATC(5/9)
482 GCATC
SphI 173 GCATGC GCATG^C
353 GCATGC
Tsp509I 253 AATT ^AATT
298 AATT
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# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AlwI BanII BbvI
BcnI BmyI BsaJI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HapII HpaII
ItaI MboI MboII MflI MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PstI RsaI Sau3AI
Sau96I ScrFI SfcI TseI TspRI
XhoII