##################################
# NO CUT ENZYMES ( 174 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Cac8I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HphI KasI
KpnI KspI MaeII MamI MfeI
MflI MluI MluNI MroI MscI
MslI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmlI SnaBI SphI Sse8387I SspBI
SspI StuI SwaI Tsp45I Tth111I
Van91I XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AlwI 29 GGATC GGATC(4/5)
AlwNI 243 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 24 CCTGAGG CC^T[ACGT]AGG
ApaI 271 GGGCCC GGGCC^C
AvaI 152 CCCGGG C^[CT]CG[GA]G
AvaII 15 GGACC G^G[AT]CC
BclI 341 TGATCA T^GATCA
BpmI 191 CTCCAG CTGGAG(16/14)
BsaI 170 GAGACC GGTCTC(1/5)
BsmAI 170 GAGAC GTCTC(1/5)
BsoBI 152 CCCGGG C^[CT]CG[GA]G
BspMI 229 ACCTGC ACCTGC(4/8)
BsrBI 18 CCGCTC CCGCTC(-3/-3)
Bsu36I 24 CCTGAGG CC^T[ACGT]AGG
DraI 817 TTTAAA TTT^AAA
Eco81I 24 CCTGAGG CC^T[ACGT]AGG
EcoNI 84 CCTTGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 479 GCGGG CCCGC(4/6)
FbaI 341 TGATCA T^GATCA
HapII 153 CCGG C^CGG
HinfI 103 GATTC G^A[ACGT]TC
HpaII 153 CCGG C^CGG
MaeIII 127 GTTAC ^GT[ACGT]AC
MspI 153 CCGG C^CGG
SmaI 152 CCCGGG CCC^GGG
SpeI 699 ACTAGT A^CTAGT
TaqI 877 TCGA T^CGA
TfiI 103 GATTC G^A[AT]TC
TthHB8I 877 TCGA T^CGA
XbaI 106 TCTAGA T^CTAGA
XmaCI 152 CCCGGG C^CCGGG
XmaI 152 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BanII 196 GGGCTC G[GA]GC[CT]^C
271 GGGCCC
BcnI 152 CCCGG CC^[GC]GG
153 CCGGG
BmyI 196 GGGCTC G[AGT]GC[ACT]^C
271 GGGCCC
BsgI 544 GTGCAG GTGCAG(16/14)
577 GTGCAG
Bsp1286I 196 GGGCTC G[AGT]GC[ACT]^C
271 GGGCCC
BsrI 49 ACTGG ACTGG(1/-1)
518 CCAGT
DpnII 30 GATC ^GATC
342 GATC
DraII 270 AGGGCCC [GA]G^G[ACGT]CC[CT]
271 GGGCCCC
EcoO109I 270 AGGGCCC [GA]G^G[ACGT]CC[CT]
271 GGGCCCC
EcoT14I 84 CCTTGG C^C[AT][AT]GG
209 CCAAGG
MboI 30 GATC ^GATC
342 GATC
MspA1I 246 CCGCTG C[AC]G^C[GT]G
486 CCGCTG
NciI 152 CCCGG CC^[GC]GG
153 CCGGG
NdeII 30 GATC ^GATC
342 GATC
Sau3AI 30 GATC ^GATC
342 GATC
StyI 84 CCTTGG C^C[AT][AT]GG
209 CCAAGG
TspRI 580 CAGTG CA[GC]TG[ACGT][ACGT]^
672 CACTG
XcmI 209 CCAAGGAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
368 CCATGAAGCACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI BbvI BfaI BsaJI
BseRI BsiYI BslI BssKI BstF5I
BstNI Cfr13I DdeI EarI EcoRII
Fnu4HI FokI HaeIII ItaI Ksp632I
MaeI MboII MnlI MseI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
SfaNI Tru9I TseI Tsp509I