Restriction map for
pF1KB8785
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcsI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BanI BanII BbeI
BcgI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MslI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PshBI PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AclI 10 AACGTT AA^CGTT
AflIII 384 ACGTGT A^C[GA][CT]GT
AvaI 188 CCCGGG C^[CT]CG[GA]G
AvaII 753 GGACC G^G[AT]CC
BamHI 27 GGATCC G^GATCC
BbrPI 383 CACGTG CAC^GTG
BbsI 733 GAAGAC GAAGAC(2/6)
BclI 806 TGATCA T^GATCA
BfaI 227 CTAG C^TAG
BmyI 421 GTGCCC G[AGT]GC[ACT]^C
BpmI 799 CTGGAG CTGGAG(16/14)
BpuAI 733 GAAGAC GAAGAC(2/6)
BsaAI 383 CACGTG [CT]AC^GT[GA]
BsgI 728 GTGCAG GTGCAG(16/14)
BsmFI 752 GGGAC GGGAC(10/14)
BsoBI 188 CCCGGG C^[CT]CG[GA]G
Bsp1286I 421 GTGCCC G[AGT]GC[ACT]^C
Bsp1407I 368 TGTACA T^GTACA
BspMI 283 GCAGGT ACCTGC(4/8)
BsrDI 405 CATTGC GCAATG(2/0)
BsrGI 368 TGTACA T^GTACA
BsrI 759 ACTGG ACTGG(1/-1)
Cfr13I 753 GGACC G^G[ACGT]CC
DraII 752 GGGACCC [GA]G^G[ACGT]CC[CT]
EarI 661 GAAGAG CTCTTC(1/4)
EcoNI 455 CCTCACGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 752 GGGACCC [GA]G^G[ACGT]CC[CT]
FbaI 806 TGATCA T^GATCA
HapII 189 CCGG C^CGG
HpaII 189 CCGG C^CGG
Ksp632I 661 GAAGAG CTCTTC(1/4)
MaeI 227 CTAG C^TAG
MaeIII 13 GTTAC ^GT[ACGT]AC
MspI 189 CCGG C^CGG
NheI 226 GCTAGC G^CTAGC
PmaCI 383 CACGTG CAC^GTG
PmlI 383 CACGTG CAC^GTG
PpuMI 752 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 10 AACGTT AA^CGTT
PvuII 796 CAGCTG CAG^CTG
Sau96I 753 GGACC G^G[ACGT]CC
SmaI 188 CCCGGG CCC^GGG
SspBI 368 TGTACA T^GTACA
TaqI 427 TCGA T^CGA
TspRI 758 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 427 TCGA T^CGA
XmaCI 188 CCCGGG C^CCGGG
XmaI 188 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AluI 679 AGCT AG^CT
797 AGCT
BalI 2 TGGCCA TGG^CCA
864 TGGCCA
BbvCI 742 GCTGAGG CCTCAGC(-5/-2)
829 GCTGAGG
BcnI 188 CCCGG CC^[GC]GG
189 CCGGG
BsaJI 188 CCCGGG C^C[ACGT][ACGT]GG
273 CCGAGG
BsiYI 249 CCTGGCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
455 CCTCACGAAGG
BslI 249 CCTGGCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
455 CCTCACGAAGG
BsmAI 697 GAGAC GTCTC(1/5)
851 GAGAC
BstXI 17 CCAACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
404 CCATTGCTCTGG
BstYI 27 GGATCC [GA]^GATC[CT]
502 GGATCT
EaeI 2 TGGCCA [CT]^GGCC[GA]
864 TGGCCA
FauI 130 GCGGG CCCGC(4/6)
498 GCGGG
HincII 434 GTCAAC GT[CT]^[GA]AC
558 GTTGAC
HindII 434 GTCAAC GT[CT]^[GA]AC
558 GTTGAC
HphI 529 GGTGA GGTGA(8/7)
642 GGTGA
MflI 27 GGATCC [GA]^GATC[CT]
502 GGATCT
MluNI 2 TGGCCA TGG^CCA
864 TGGCCA
MscI 2 TGGCCA TGG^CCA
864 TGGCCA
MspA1I 302 CAGCGG C[AC]G^C[GT]G
796 CAGCTG
NciI 188 CCCGG CC^[GC]GG
189 CCGGG
SfcI 197 CTGTAG C^T[GA][CT]AG
416 CTGTAG
TfiI 63 GAATC G^A[AT]TC
583 GATTC
XhoII 27 GGATCC [GA]^GATC[CT]
502 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AfaI AlwI BbvI BseRI
BssKI BstF5I BstNI Cac8I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HinfI ItaI MaeII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
ScrFI Tru9I TseI Tsp509I