##################################
# NO CUT ENZYMES (  151 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       Aor51HI     
ApaLI       ApoI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BamHI       BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BsiWI       BsmAI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrDI       
BsrGI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstYI       BstZ17I     
CelII       ClaI        CpoI        DpnII       DraI        
DraIII      Eam1105I    Eco47III    EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HinfI       
HpaI        KpnI        MaeI        MaeII       MamI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MslI        MunI        
NdeI        NdeII       NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsaI        RsrII       
SalI        Sau3AI      SbfI        ScaI        SexAI       
SfiI        SfuI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        TaqI        
TfiI        Tru9I       Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AocI          276  CCTGAGG              CC^T[ACGT]AGG
ApaI          623  GGGCCC               GGGCC^C
AspHI         321  GAGCTC               G[AT]GC[AT]^C
BglI          602  GCCGTGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI           21  CTGCAC               GTGCAG(16/14)
BsiEI         114  CGGCCG               CG[GA][CT]^CG
BsiHKAI       321  GAGCTC               G[AT]GC[AT]^C
Bsu36I        276  CCTGAGG              CC^T[ACGT]AGG
DdeI          277  CTGAG                C^T[ACGT]AG
DrdI          673  GACGGCTGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          114  CGGCCG               C^GGCCG
EarI          520  GAAGAG               CTCTTC(1/4)
EclXI         114  CGGCCG               C^GGCCG
Eco52I        114  CGGCCG               C^GGCCG
Eco81I        276  CCTGAGG              CC^T[ACGT]AGG
EcoT14I        26  CCATGG               C^C[AT][AT]GG
HphI          664  GGTGA                GGTGA(8/7)
Ksp632I       520  GAAGAG               CTCTTC(1/4)
MaeIII        665  GTGAC                ^GT[ACGT]AC
MboII         520  GAAGA                GAAGA(8/7)
NcoI           26  CCATGG               C^CATGG
NlaIII         27  CATG                 CATG^
SacI          321  GAGCTC               GAGCT^C
SapI          520  GAAGAGC              GCTCTTC(1/4)
SfaNI         180  GCATC                GCATC(5/9)
SgrAI         289  CACCGGCG             C[GA]^CCGG[CT]G
SmlI          646  CTCAAG               C^T[CT][GA]AG
StuI          140  AGGCCT               AGG^CCT
StyI           26  CCATGG               C^C[AT][AT]GG
Tsp45I        665  GTGAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AscI          387  GGCGCGCC             GG^CGCGCC
              540  GGCGCGCC            
BanII         321  GAGCTC               G[GA]GC[CT]^C
              623  GGGCCC              
BmyI          321  GAGCTC               G[AGT]GC[ACT]^C
              623  GGGCCC              
BseRI           3  GAGGAG               GAGGAG(10/8)
              279  GAGGAG              
Bsp1286I      321  GAGCTC               G[AGT]GC[ACT]^C
              623  GGGCCC              
BstF5I        181  CATCC                GGATG(2/0)
              591  GGATG               
FokI          181  CATCC                GGATG(9/13)
              591  GGATG               
PpuMI         253  GGGACCT              [GA]G^G[AT]CC[CT]
              692  AGGTCCT             
SmaI          175  CCCGGG               CCC^GGG
              237  CCCGGG              
TspRI          73  CAGTG                CA[GC]TG[ACGT][ACGT]^
              193  CACTG               
XmaCI         175  CCCGGG               C^CCGGG
              237  CCCGGG              
XmaI          175  CCCGGG               C^CCGGG
              237  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AvaI        
AvaII       BanI        BbeI        BbvI        BcnI        
BpmI        BsaHI       BsaJI       BsiYI       BslI        
BsmFI       BsoBI       BspMI       BsrBI       BsrFI       
BsrI        BssHII      BssKI       BstNI       BstUI       
Cac8I       CfoI        Cfr10I      Cfr13I      DraII       
DsaI        EaeI        EcoO109I    EcoRII      FauI        
Fnu4HI      HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HpaII       ItaI        
KasI        KspI        MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NgoMIV      NlaIV       PstI        SacII       
Sau96I      ScrFI       SfcI        SfoI        TseI