Restriction map for
pF1KB8763
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI AlwI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BsaWI BseAI BsgI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrGI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DpnII DraI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FspI HincII
HindII HindIII HpaI KpnI MaeI
MaeIII MamI MboI MfeI MflI
MluI MroI MseI MunI NcoI
NdeI NdeII NheI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SalI SapI
Sau3AI SbfI ScaI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI StyI
SwaI Tru9I Tsp45I Tsp509I TspRI
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AlwNI 523 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 360 CTCGGG C^[CT]CG[GA]G
BalI 548 TGGCCA TGG^CCA
BbeI 468 GGCGCC GGCGC^C
BglI 54 GCCCCGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 451 CTGGAG CTGGAG(16/14)
BsaAI 460 TACGTG [CT]AC^GT[GA]
BsmAI 37 GTCTC GTCTC(1/5)
BsoBI 360 CTCGGG C^[CT]CG[GA]G
BsrBI 271 GAGCGG CCGCTC(-3/-3)
BsrI 239 ACTGG ACTGG(1/-1)
DraIII 457 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 28 GACAATGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI 393 GGCCGGCC GGCCGG^CC
HgaI 223 GACGC GACGC(5/10)
HinfI 233 GATTC G^A[ACGT]TC
HphI 405 TCACC GGTGA(8/7)
KasI 468 GGCGCC G^GCGCC
MaeII 461 ACGT A^CGT
MluNI 548 TGGCCA TGG^CCA
MscI 548 TGGCCA TGG^CCA
NaeI 394 GCCGGC GCC^GGC
NarI 468 GGCGCC GG^CGCC
NgoMIV 394 GCCGGC G^CCGGC
NlaIII 480 CATG CATG^
NotI 513 GCGGCCGC GC^GGCCGC
NspI 479 GCATGC [GA]CATG^[CT]
PpuMI 363 GGGACCC [GA]G^G[AT]CC[CT]
PstI 520 CTGCAG CTGCA^G
SacI 612 GAGCTC GAGCT^C
SexAI 14 ACCAGGT A^CC[AT]GGT
SfcI 520 CTGCAG C^T[GA][CT]AG
SfoI 468 GGCGCC GGC^GCC
SphI 479 GCATGC GCATG^C
TfiI 233 GATTC G^A[AT]TC
XcmI 209 CCAGCGCGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 223 GACGCC G[GA]^CG[CT]C
468 GGCGCC
Alw44I 256 GTGCAC G^TGCAC
382 GTGCAC
ApaI 244 GGGCCC GGGCC^C
488 GGGCCC
ApaLI 256 GTGCAC G^TGCAC
382 GTGCAC
AvaII 132 GGTCC G^G[AT]CC
364 GGACC
BanI 295 GGCACC G^G[CT][GA]CC
468 GGCGCC
BsaHI 223 GACGCC G[GA]^CG[CT]C
468 GGCGCC
BsmFI 363 GGGAC GGGAC(10/14)
133 GTCCC
BsrFI 191 ACCGGC [GA]^CCGG[CT]
394 GCCGGC
BssSI 268 CACGAG CACGAG(-5/-1)
369 CACGAG
Cfr10I 191 ACCGGC [GA]^CCGG[CT]
394 GCCGGC
EarI 81 CTCTTC CTCTTC(1/4)
439 CTCTTC
HaeII 468 GGCGCC [GA]GCGC^[CT]
503 AGCGCC
Hin1I 223 GACGCC G[GA]^CG[CT]C
468 GGCGCC
Ksp632I 81 CTCTTC CTCTTC(1/4)
439 CTCTTC
MslI 375 CATCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
406 CACCTGGATG
TaqI 431 TCGA T^CGA
443 TCGA
TthHB8I 431 TCGA T^CGA
443 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AluI AspHI BanII
BbvI BcnI BmyI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI Bsp1286I
BssHII BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
ItaI KspI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIV SacII Sau96I ScrFI SfaNI
TseI