################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I AlwNI AocI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlpI Bpu1102I BpuAI BsaWI BseAI BsiHKAI BsiWI BsmI BspDI BspEI BspHI BspMI BsrBI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DrdI Eam1105I Eco81I EcoNI EcoO65I EcoRV FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MfeI MluI MroI MunI NdeI NheI NotI NruI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I Van91I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AflIII 913 ACATGT A^C[GA][CT]GT Aor51HI 422 AGCGCT AGC^GCT ApaI 86 GGGCCC GGGCC^C AvrII 948 CCTAGG C^CTAGG BamHI 1014 GGATCC G^GATCC BbeI 243 GGCGCC GGCGC^C BlnI 948 CCTAGG C^CTAGG BpmI 992 CTCCAG CTGGAG(16/14) BsaAI 136 TACGTG [CT]AC^GT[GA] BsaBI 633 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 254 GAGACC GGTCTC(1/5) BsmBI 171 CGTCTC CGTCTC(1/5) BsmFI 235 GTCCC GGGAC(10/14) BspLU11I 913 ACATGT A^CATGT BsrFI 1162 GCCGGC [GA]^CCGG[CT] BssSI 341 CTCGTG CACGAG(-5/-1) BstF5I 972 GGATG GGATG(2/0) BstYI 1014 GGATCC [GA]^GATC[CT] Cfr10I 1162 GCCGGC [GA]^CCGG[CT] DraI 1123 TTTAAA TTT^AAA DraIII 1106 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 503 GAAGAG CTCTTC(1/4) Eco47III 422 AGCGCT AGC^GCT EcoT22I 882 ATGCAT ATGCA^T FokI 972 GGATG GGATG(9/13) KasI 243 GGCGCC G^GCGCC Ksp632I 503 GAAGAG CTCTTC(1/4) MaeII 137 ACGT A^CGT MamI 633 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1014 GGATCC [GA]^GATC[CT] MseI 1124 TTAA T^TAA NaeI 1162 GCCGGC GCC^GGC NarI 243 GGCGCC GG^CGCC NcoI 892 CCATGG C^CATGG NgoMIV 1162 GCCGGC G^CCGGC NsiI 882 ATGCAT ATGCA^T PpuMI 1188 AGGACCC [GA]G^G[AT]CC[CT] PvuII 1019 CAGCTG CAG^CTG SfoI 243 GGCGCC GGC^GCC SspI 657 AATATT AAT^ATT Tru9I 1124 TTAA T^TAA Tsp45I 1172 GTGAC ^GT[GC]AC XhoII 1014 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AcyI 232 GGCGTC G[GA]^CG[CT]C 243 GGCGCC AvaII 141 GGACC G^G[AT]CC 1189 GGACC BalI 611 TGGCCA TGG^CCA 741 TGGCCA BanI 243 GGCGCC G^G[CT][GA]CC 562 GGCACC BsaHI 232 GGCGTC G[GA]^CG[CT]C 243 GGCGCC BseRI 1001 CTCCTC GAGGAG(10/8) 418 GAGGAG BsgI 351 CTGCAC GTGCAG(16/14) 806 GTGCAG Bsp1407I 573 TGTACA T^GTACA 975 TGTACA BsrDI 615 CATTGC GCAATG(2/0) 1151 CATTGC BsrGI 573 TGTACA T^GTACA 975 TGTACA BsrI 688 ACTGG ACTGG(1/-1) 538 CCAGT BssHII 215 GCGCGC G^CGCGC 269 GCGCGC DraII 85 GGGGCCC [GA]G^G[ACGT]CC[CT] 1188 AGGACCC EcoO109I 85 GGGGCCC [GA]G^G[ACGT]CC[CT] 1188 AGGACCC EcoRI 533 GAATTC G^AATTC 848 GAATTC FauI 144 CCCGC CCCGC(4/6) 457 GCGGG HaeII 243 GGCGCC [GA]GCGC^[CT] 422 AGCGCT Hin1I 232 GGCGTC G[GA]^CG[CT]C 243 GGCGCC MaeIII 541 GTAAC ^GT[ACGT]AC 1172 GTGAC MboII 503 GAAGA GAAGA(8/7) 287 TCTTC MluNI 611 TGGCCA TGG^CCA 741 TGGCCA MscI 611 TGGCCA TGG^CCA 741 TGGCCA MslI 624 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 627 CACCATGATG MspA1I 588 CAGCGG C[AC]G^C[GT]G 1019 CAGCTG NspI 570 GCATGT [GA]CATG^[CT] 913 ACATGT PaeR7I 103 CTCGAG C^TCGAG 259 CTCGAG PleI 1174 GACTC GAGTC(4/5) 1032 GAGTC SmaI 82 CCCGGG CCC^GGG 89 CCCGGG SmlI 103 CTCGAG C^T[CT][GA]AG 259 CTCGAG SspBI 573 TGTACA T^GTACA 975 TGTACA TfiI 997 GAATC G^A[AT]TC 1096 GAATC TspRI 809 CAGTG CA[GC]TG[ACGT][ACGT]^ 1108 CAGTG XhoI 103 CTCGAG C^TCGAG 259 CTCGAG XmaCI 82 CCCGGG C^CCGGG 89 CCCGGG XmaI 82 CCCGGG C^CCGGG 89 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI AvaI BanII BbvI BcnI BfaI BglI BmyI BsaJI BsiEI BsiYI BslI BsmAI BsoBI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EagI EclXI Eco52I EcoRII EcoT14I Fnu4HI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeI MboI MnlI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI Tsp509I TthHB8I