Restriction map for pF1KB8710
##################################
# NO CUT ENZYMES (  113 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        AhdI        Alw44I      AlwNI       
AocI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AviII       BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfrI        BglII       BlpI        
Bpu1102I    BpuAI       BsaWI       BseAI       BsiHKAI     
BsiWI       BsmI        BspDI       BspEI       BspHI       
BspMI       BsrBI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DrdI        Eam1105I    
Eco81I      EcoNI       EcoO65I     EcoRV       FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        KspI        MfeI        MluI        
MroI        MunI        NdeI        NheI        NotI        
NruI        NspV        PacI        PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    StuI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AflIII        913  ACATGT               A^C[GA][CT]GT
Aor51HI       422  AGCGCT               AGC^GCT
ApaI           86  GGGCCC               GGGCC^C
AvrII         948  CCTAGG               C^CTAGG
BamHI        1014  GGATCC               G^GATCC
BbeI          243  GGCGCC               GGCGC^C
BlnI          948  CCTAGG               C^CTAGG
BpmI          992  CTCCAG               CTGGAG(16/14)
BsaAI         136  TACGTG               [CT]AC^GT[GA]
BsaBI         633  GATGATTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          254  GAGACC               GGTCTC(1/5)
BsmBI         171  CGTCTC               CGTCTC(1/5)
BsmFI         235  GTCCC                GGGAC(10/14)
BspLU11I      913  ACATGT               A^CATGT
BsrFI        1162  GCCGGC               [GA]^CCGG[CT]
BssSI         341  CTCGTG               CACGAG(-5/-1)
BstF5I        972  GGATG                GGATG(2/0)
BstYI        1014  GGATCC               [GA]^GATC[CT]
Cfr10I       1162  GCCGGC               [GA]^CCGG[CT]
DraI         1123  TTTAAA               TTT^AAA
DraIII       1106  CACAGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          503  GAAGAG               CTCTTC(1/4)
Eco47III      422  AGCGCT               AGC^GCT
EcoT22I       882  ATGCAT               ATGCA^T
FokI          972  GGATG                GGATG(9/13)
KasI          243  GGCGCC               G^GCGCC
Ksp632I       503  GAAGAG               CTCTTC(1/4)
MaeII         137  ACGT                 A^CGT
MamI          633  GATGATTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI         1014  GGATCC               [GA]^GATC[CT]
MseI         1124  TTAA                 T^TAA
NaeI         1162  GCCGGC               GCC^GGC
NarI          243  GGCGCC               GG^CGCC
NcoI          892  CCATGG               C^CATGG
NgoMIV       1162  GCCGGC               G^CCGGC
NsiI          882  ATGCAT               ATGCA^T
PpuMI        1188  AGGACCC              [GA]G^G[AT]CC[CT]
PvuII        1019  CAGCTG               CAG^CTG
SfoI          243  GGCGCC               GGC^GCC
SspI          657  AATATT               AAT^ATT
Tru9I        1124  TTAA                 T^TAA
Tsp45I       1172  GTGAC                ^GT[GC]AC
XhoII        1014  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AcyI          232  GGCGTC               G[GA]^CG[CT]C
              243  GGCGCC              
AvaII         141  GGACC                G^G[AT]CC
             1189  GGACC               
BalI          611  TGGCCA               TGG^CCA
              741  TGGCCA              
BanI          243  GGCGCC               G^G[CT][GA]CC
              562  GGCACC              
BsaHI         232  GGCGTC               G[GA]^CG[CT]C
              243  GGCGCC              
BseRI        1001  CTCCTC               GAGGAG(10/8)
              418  GAGGAG              
BsgI          351  CTGCAC               GTGCAG(16/14)
              806  GTGCAG              
Bsp1407I      573  TGTACA               T^GTACA
              975  TGTACA              
BsrDI         615  CATTGC               GCAATG(2/0)
             1151  CATTGC              
BsrGI         573  TGTACA               T^GTACA
              975  TGTACA              
BsrI          688  ACTGG                ACTGG(1/-1)
              538  CCAGT               
BssHII        215  GCGCGC               G^CGCGC
              269  GCGCGC              
DraII          85  GGGGCCC              [GA]G^G[ACGT]CC[CT]
             1188  AGGACCC             
EcoO109I       85  GGGGCCC              [GA]G^G[ACGT]CC[CT]
             1188  AGGACCC             
EcoRI         533  GAATTC               G^AATTC
              848  GAATTC              
FauI          144  CCCGC                CCCGC(4/6)
              457  GCGGG               
HaeII         243  GGCGCC               [GA]GCGC^[CT]
              422  AGCGCT              
Hin1I         232  GGCGTC               G[GA]^CG[CT]C
              243  GGCGCC              
MaeIII        541  GTAAC                ^GT[ACGT]AC
             1172  GTGAC               
MboII         503  GAAGA                GAAGA(8/7)
              287  TCTTC               
MluNI         611  TGGCCA               TGG^CCA
              741  TGGCCA              
MscI          611  TGGCCA               TGG^CCA
              741  TGGCCA              
MslI          624  CATCACCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              627  CACCATGATG          
MspA1I        588  CAGCGG               C[AC]G^C[GT]G
             1019  CAGCTG              
NspI          570  GCATGT               [GA]CATG^[CT]
              913  ACATGT              
PaeR7I        103  CTCGAG               C^TCGAG
              259  CTCGAG              
PleI         1174  GACTC                GAGTC(4/5)
             1032  GAGTC               
SmaI           82  CCCGGG               CCC^GGG
               89  CCCGGG              
SmlI          103  CTCGAG               C^T[CT][GA]AG
              259  CTCGAG              
SspBI         573  TGTACA               T^GTACA
              975  TGTACA              
TfiI          997  GAATC                G^A[AT]TC
             1096  GAATC               
TspRI         809  CAGTG                CA[GC]TG[ACGT][ACGT]^
             1108  CAGTG               
XhoI          103  CTCGAG               C^TCGAG
              259  CTCGAG              
XmaCI          82  CCCGGG               C^CCGGG
               89  CCCGGG              
XmaI           82  CCCGGG               C^CCGGG
               89  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        ApoI        AvaI        BanII       BbvI        
BcnI        BfaI        BglI        BmyI        BsaJI       
BsiEI       BsiYI       BslI        BsmAI       BsoBI       
Bsp1286I    BssKI       BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DsaI        
EaeI        EagI        EclXI       Eco52I      EcoRII      
EcoT14I     Fnu4HI      HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeI        MboI        MnlI        MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TaqI        TseI        
Tsp509I     TthHB8I     
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