Restriction map for
pF1KB8702
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
AlwI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspI AviII
AvrII BamHI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BsaAI BsaBI BsaI BsaWI
BseAI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssSI Bst1107I
BstBI BstEII BstF5I BstPI BstYI
BstZ17I ClaI CpoI DpnII DraI
Eco47III EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FokI FseI FspI
HindIII HpaI KpnI MaeI MamI
MboI MfeI MflI MluI MroI
MseI MunI NcoI NdeI NdeII
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI Sau3AI SbfI
SexAI SfaNI SfiI SfuI SnaBI
SpeI Sse8387I SspBI SspI StyI
SwaI TfiI Tru9I Tsp509I Tth111I
XbaI XcmI XhoII
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# ONE CUT ENZYMES ( 40 enzymes) #
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AfaI 891 GTAC GT^AC
AhdI 1103 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1097 CCTCAGG CC^T[ACGT]AGG
Asp700I 102 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1103 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1033 TGGCCA TGG^CCA
BbsI 607 GTCTTC GAAGAC(2/6)
BlpI 1048 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1048 GCTGAGC GC^T[ACGT]AGC
BpuAI 607 GTCTTC GAAGAC(2/6)
BspMI 1020 GCAGGT ACCTGC(4/8)
BsrI 966 CCAGT ACTGG(1/-1)
BstXI 365 CCAGCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1097 CCTCAGG CC^T[ACGT]AGG
CelII 1048 GCTGAGC GC^T[ACGT]AGC
DraII 45 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 649 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 790 GACGTGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1103 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1097 CCTCAGG CC^T[ACGT]AGG
EcoO109I 45 GGGACCC [GA]G^G[ACGT]CC[CT]
HincII 1041 GTTGAC GT[CT]^[GA]AC
HindII 1041 GTTGAC GT[CT]^[GA]AC
HinfI 1109 GAGTC G^A[ACGT]TC
KspI 380 CCGCGG CCGC^GG
MluNI 1033 TGGCCA TGG^CCA
MscI 1033 TGGCCA TGG^CCA
MslI 612 CACGCGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 617 GCATGC [GA]CATG^[CT]
PleI 1109 GAGTC GAGTC(4/5)
PpuMI 45 GGGACCC [GA]G^G[AT]CC[CT]
RsaI 891 GTAC GT^AC
SacI 949 GAGCTC GAGCT^C
SacII 380 CCGCGG CCGC^GG
SapI 237 GCTCTTC GCTCTTC(1/4)
ScaI 890 AGTACT AGT^ACT
SgrAI 591 CACCGGCG C[GA]^CCGG[CT]G
SphI 617 GCATGC GCATG^C
StuI 18 AGGCCT AGG^CCT
XmnI 102 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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ApaI 247 GGGCCC GGGCC^C
697 GGGCCC
AvaII 46 GGACC G^G[AT]CC
798 GGTCC
BglI 20 GCCTGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
715 GCCCTACCGGC
BseRI 832 CTCCTC GAGGAG(10/8)
946 GAGGAG
BsgI 391 CTGCAC GTGCAG(16/14)
655 GTGCAG
BstAPI 7 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
657 GCAGTCACTGC
DdeI 1049 CTGAG C^T[ACGT]AG
1098 CTCAG
EarI 238 CTCTTC CTCTTC(1/4)
286 CTCTTC
EcoNI 267 CCTCACCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
291 CCTGCGGCAGG
HphI 269 TCACC GGTGA(8/7)
1068 TCACC
Ksp632I 238 CTCTTC CTCTTC(1/4)
286 CTCTTC
MaeII 125 ACGT A^CGT
791 ACGT
MaeIII 488 GTCAC ^GT[ACGT]AC
660 GTCAC
NlaIII 618 CATG CATG^
978 CATG
PaeR7I 397 CTCGAG C^TCGAG
436 CTCGAG
SmlI 397 CTCGAG C^T[CT][GA]AG
436 CTCGAG
Tsp45I 488 GTCAC ^GT[GC]AC
660 GTCAC
TspRI 662 CACTG CA[GC]TG[ACGT][ACGT]^
1037 CACTG
XhoI 397 CTCGAG C^TCGAG
436 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AspHI AvaI BanI BanII BbeI
BbvI BcnI BmyI BpmI BsaHI
BsaJI BsiEI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrFI BssHII
BssKI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DsaI EaeI EagI
EclXI Eco52I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII ItaI KasI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NgoMIV NlaIV PflMI PstI PvuII
Sau96I ScrFI SfcI SfoI SmaI
TaqI TseI TthHB8I Van91I XmaCI
XmaI