Restriction map for
pF1KB8645
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvrII BamHI
BbeI BbrPI BbvCI BcgI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstPI BstXI Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HindIII HpaI KasI KpnI MaeI
MamI MfeI MluI MroI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SalI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp509I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 175 GTATAC GT^[AC][GT]AC
AflIII 194 ACATGT A^C[GA][CT]GT
Alw44I 786 GTGCAC G^TGCAC
AlwI 345 GATCC GGATC(4/5)
AlwNI 343 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 786 GTGCAC G^TGCAC
AspI 9 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 83 CTCGGG C^[CT]CG[GA]G
AviII 248 TGCGCA TGC^GCA
BalI 566 TGGCCA TGG^CCA
BciVI 216 GGATAC GTATCC(6/5)
BcnI 306 CCGGG CC^[GC]GG
BglII 608 AGATCT A^GATCT
BpmI 349 CTGGAG CTGGAG(16/14)
BsaAI 550 TACGTG [CT]AC^GT[GA]
BsaI 690 GGTCTC GGTCTC(1/5)
BsmFI 779 GGGAC GGGAC(10/14)
BsoBI 83 CTCGGG C^[CT]CG[GA]G
BspLU11I 194 ACATGT A^CATGT
BsrI 138 CCAGT ACTGG(1/-1)
BssSI 580 CACGAG CACGAG(-5/-1)
Bst1107I 175 GTATAC GTA^TAC
BstZ17I 175 GTATAC GTA^TAC
DdeI 34 CTCAG C^T[ACGT]AG
EarI 556 CTCTTC CTCTTC(1/4)
FspI 248 TGCGCA TGC^GCA
HaeII 329 AGCGCC [GA]GCGC^[CT]
HapII 306 CCGG C^CGG
HincII 718 GTCAAC GT[CT]^[GA]AC
HindII 718 GTCAAC GT[CT]^[GA]AC
HinfI 310 GAGTC G^A[ACGT]TC
HpaII 306 CCGG C^CGG
HphI 625 GGTGA GGTGA(8/7)
Ksp632I 556 CTCTTC CTCTTC(1/4)
KspI 186 CCGCGG CCGC^GG
MluNI 566 TGGCCA TGG^CCA
MscI 566 TGGCCA TGG^CCA
MspI 306 CCGG C^CGG
NciI 306 CCGGG CC^[GC]GG
NcoI 401 CCATGG C^CATGG
PflFI 9 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 310 GAGTC GAGTC(4/5)
PpuMI 749 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 96 GAGCTC GAGCT^C
SacII 186 CCGCGG CCGC^GG
SapI 555 GCTCTTC GCTCTTC(1/4)
SfaNI 600 GCATC GCATC(5/9)
SmlI 730 CTCAAG C^T[CT][GA]AG
SphI 653 GCATGC GCATG^C
Tth111I 9 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 187 CGCG CG^CG
661 CGCG
AcyI 628 GACGCC G[GA]^CG[CT]C
676 GACGCC
AvaII 135 GGACC G^G[AT]CC
750 GGTCC
BanI 76 GGTGCC G^G[CT][GA]CC
450 GGTGCC
BanII 96 GAGCTC G[GA]GC[CT]^C
588 GAGCCC
BbsI 6 GAAGAC GAAGAC(2/6)
277 GTCTTC
BpuAI 6 GAAGAC GAAGAC(2/6)
277 GTCTTC
BsaHI 628 GACGCC G[GA]^CG[CT]C
676 GACGCC
BsgI 27 CTGCAC GTGCAG(16/14)
616 GTGCAG
BsmAI 108 GTCTC GTCTC(1/5)
691 GTCTC
BstUI 187 CGCG CG^CG
661 CGCG
BstYI 344 AGATCC [GA]^GATC[CT]
608 AGATCT
DpnII 345 GATC ^GATC
609 GATC
DraII 643 AGGCCCT [GA]G^G[ACGT]CC[CT]
749 AGGTCCT
EcoO109I 643 AGGCCCT [GA]G^G[ACGT]CC[CT]
749 AGGTCCT
Hin1I 628 GACGCC G[GA]^CG[CT]C
676 GACGCC
MaeII 533 ACGT A^CGT
551 ACGT
MaeIII 626 GTGAC ^GT[ACGT]AC
725 GTGAC
MboI 345 GATC ^GATC
609 GATC
MflI 344 AGATCC [GA]^GATC[CT]
608 AGATCT
MspA1I 18 CAGCGG C[AC]G^C[GT]G
186 CCGCGG
MvnI 187 CGCG CG^CG
661 CGCG
NdeII 345 GATC ^GATC
609 GATC
NlaIV 76 GGTGCC GG[ACGT]^[ACGT]CC
450 GGTGCC
NspI 194 ACATGT [GA]CATG^[CT]
653 GCATGC
Sau3AI 345 GATC ^GATC
609 GATC
Tsp45I 626 GTGAC ^GT[GC]AC
725 GTGAC
TspRI 36 CAGTG CA[GC]TG[ACGT][ACGT]^
723 CAGTG
XhoII 344 AGATCC [GA]^GATC[CT]
608 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AfaI AluI AspHI BbvI
BmyI BsaJI BsiHKAI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DsaI EaeI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HgaI HhaI HinP1I ItaI MboII
MnlI MslI MvaI MwoI NlaIII
RsaI Sau96I ScrFI StyI TaqI
TseI TthHB8I