Restriction map for
pF1KB8642
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccII AccIII AcyI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AvaI AviII AvrII
BanII BbeI BbrPI BcgI BcnI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspEI
BspLU11I BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoT22I FseI
FspI HapII HgaI Hin1I HpaI
HpaII KasI KpnI KspI MluI
MroI MspI MvnI NaeI NarI
NciI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PvuI
PvuII RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 3055 GTCTAC GT^[AC][GT]AC
AflII 2389 CTTAAG C^TTAAG
AlwNI 554 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 2 TGGCCA TGG^CCA
BamHI 307 GGATCC G^GATCC
BanI 2114 GGTGCC G^G[CT][GA]CC
BbvCI 1087 GCTGAGG CCTCAGC(-5/-2)
BciVI 2478 GGATAC GTATCC(6/5)
BfrI 2389 CTTAAG C^TTAAG
BsaBI 1918 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2698 GAGACC GGTCTC(1/5)
BspDI 2615 ATCGAT AT^CGAT
BspMI 942 GCAGGT ACCTGC(4/8)
BsrBI 724 GAGCGG CCGCTC(-3/-3)
BsrDI 3227 CATTGC GCAATG(2/0)
BstAPI 2638 GCAGAACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 307 GGATCC [GA]^GATC[CT]
CfoI 744 GCGC GCG^C
ClaI 2615 ATCGAT AT^CGAT
DraIII 2985 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 3188 GAATTC G^AATTC
EcoRV 352 GATATC GAT^ATC
EcoT14I 3086 CCATGG C^C[AT][AT]GG
FauI 2853 GCGGG CCCGC(4/6)
HaeII 743 AGCGCC [GA]GCGC^[CT]
HhaI 744 GCGC GCG^C
HinP1I 744 GCGC G^CGC
HincII 211 GTCAAC GT[CT]^[GA]AC
HindII 211 GTCAAC GT[CT]^[GA]AC
HindIII 1904 AAGCTT A^AGCTT
MamI 1918 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 865 CAATTG C^AATTG
MflI 307 GGATCC [GA]^GATC[CT]
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MspA1I 2851 CAGCGG C[AC]G^C[GT]G
MunI 865 CAATTG C^AATTG
NcoI 3086 CCATGG C^CATGG
NdeI 2272 CATATG CA^TATG
NheI 2376 GCTAGC G^CTAGC
PflMI 1188 CCAATCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SphI 1368 GCATGC GCATG^C
SspI 2158 AATATT AAT^ATT
StyI 3086 CCATGG C^C[AT][AT]GG
Van91I 1188 CCAATCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 307 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
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AclI 767 AACGTT AA^CGTT
2972 AACGTT
AseI 2359 ATTAAT AT^TAAT
2611 ATTAAT
AsnI 2359 ATTAAT AT^TAAT
2611 ATTAAT
Asp700I 766 GAACGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1294 GAACAGTTTC
AspHI 1025 GAGCAC G[AT]GC[AT]^C
2770 GAGCAC
BbsI 94 GAAGAC GAAGAC(2/6)
801 GAAGAC
BclI 525 TGATCA T^GATCA
1923 TGATCA
BmyI 1025 GAGCAC G[AGT]GC[ACT]^C
2770 GAGCAC
BpmI 2918 CTGGAG CTGGAG(16/14)
2963 CTGGAG
BpuAI 94 GAAGAC GAAGAC(2/6)
801 GAAGAC
BseRI 377 GAGGAG GAGGAG(10/8)
2530 GAGGAG
BsgI 248 GTGCAG GTGCAG(16/14)
1862 GTGCAG
BsiHKAI 1025 GAGCAC G[AT]GC[AT]^C
2770 GAGCAC
BsmI 870 GAATGC GAATGC(1/-1)
1532 GCATTC
Bsp1286I 1025 GAGCAC G[AGT]GC[ACT]^C
2770 GAGCAC
BspHI 629 TCATGA T^CATGA
1643 TCATGA
DsaI 485 CCGTGG C^C[GA][CT]GG
3086 CCATGG
EaeI 2 TGGCCA [CT]^GGCC[GA]
1675 CGGCCA
EcoNI 2917 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3208 CCTGAAAGAGG
FbaI 525 TGATCA T^GATCA
1923 TGATCA
NspI 1368 GCATGC [GA]CATG^[CT]
2643 ACATGC
PshBI 2359 ATTAAT AT^TAAT
2611 ATTAAT
Psp1406I 767 AACGTT AA^CGTT
2972 AACGTT
RcaI 629 TCATGA T^CATGA
1643 TCATGA
ScaI 252 AGTACT AGT^ACT
1180 AGTACT
StuI 1253 AGGCCT AGG^CCT
1595 AGGCCT
XmnI 766 GAACGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1294 GAACAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BfaI BsaJI
BsiYI BslI BsmAI BsmFI BsrI
BssKI BssSI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV PleI PpuMI
PstI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I