Restriction map for
pF1KB8638
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AgeI
AhdI Alw44I AlwI AocI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbsI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspMI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FauI
FbaI FseI FspI HgaI Hin1I
HpaI KasI KpnI KspI MaeI
MaeII MamI MfeI MflI MluI
MroI MunI MvnI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AciI 1059 CCGC CCGC(-3/-1)
AflIII 101 ACATGT A^C[GA][CT]GT
AlwNI 824 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 848 AGCGCT AGC^GCT
BalI 513 TGGCCA TGG^CCA
BbvCI 1048 GCTGAGG CCTCAGC(-5/-2)
BcgI 31 GCAGAAATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 1080 GTGCCC G[AGT]GC[ACT]^C
BpmI 844 CTGGAG CTGGAG(16/14)
BsaI 1000 GAGACC GGTCTC(1/5)
BsaWI 666 TCCGGT [AT]^CCGG[AT]
BseRI 700 GAGGAG GAGGAG(10/8)
BsgI 938 GTGCAG GTGCAG(16/14)
BsmAI 1000 GAGAC GTCTC(1/5)
BsmFI 1092 GGGAC GGGAC(10/14)
BsmI 321 GAATGC GAATGC(1/-1)
Bsp1286I 1080 GTGCCC G[AGT]GC[ACT]^C
BspLU11I 101 ACATGT A^CATGT
BsrBI 1059 CCGCTC CCGCTC(-3/-3)
CfoI 849 GCGC GCG^C
DraII 1092 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1039 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 893 CCGTGG C^C[GA][CT]GG
EarI 469 CTCTTC CTCTTC(1/4)
Eco47III 848 AGCGCT AGC^GCT
EcoO109I 1092 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 326 CCAAGG C^C[AT][AT]GG
HaeII 848 AGCGCT [GA]GCGC^[CT]
HhaI 849 GCGC GCG^C
HinP1I 849 GCGC G^CGC
HindIII 460 AAGCTT A^AGCTT
Ksp632I 469 CTCTTC CTCTTC(1/4)
MluNI 513 TGGCCA TGG^CCA
MscI 513 TGGCCA TGG^CCA
MslI 525 CATGAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 101 ACATGT [GA]CATG^[CT]
PflMI 1038 CCACTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 354 GAGTC GAGTC(4/5)
PpuMI 1092 GGGACCC [GA]G^G[AT]CC[CT]
SfaNI 871 GATGC GCATC(5/9)
StyI 326 CCAAGG C^C[AT][AT]GG
Van91I 1038 CCACTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AvaII 107 GGACC G^G[AT]CC
1093 GGACC
BcnI 132 CCGGG CC^[GC]GG
590 CCCGG
BsrFI 551 GCCGGC [GA]^CCGG[CT]
619 GCCGGC
BsrI 267 ACTGG ACTGG(1/-1)
389 ACTGG
BstXI 341 CCAAAGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
524 CCATGAACATGG
Cfr10I 551 GCCGGC [GA]^CCGG[CT]
619 GCCGGC
DpnII 78 GATC ^GATC
943 GATC
EcoRI 585 GAATTC G^AATTC
628 GAATTC
HincII 356 GTCAAC GT[CT]^[GA]AC
502 GTCAAC
HindII 356 GTCAAC GT[CT]^[GA]AC
502 GTCAAC
HphI 145 GGTGA GGTGA(8/7)
480 GGTGA
MboI 78 GATC ^GATC
943 GATC
MspA1I 273 CAGCTG C[AC]G^C[GT]G
824 CAGCTG
MwoI 637 GCTCACAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
646 GCTATCTTAGC
NaeI 551 GCCGGC GCC^GGC
619 GCCGGC
NciI 132 CCGGG CC^[GC]GG
590 CCCGG
NdeII 78 GATC ^GATC
943 GATC
NgoMIV 551 GCCGGC G^CCGGC
619 GCCGGC
PstI 879 CTGCAG CTGCA^G
905 CTGCAG
PvuII 273 CAGCTG CAG^CTG
824 CAGCTG
Sau3AI 78 GATC ^GATC
943 GATC
SfcI 879 CTGCAG C^T[GA][CT]AG
905 CTGCAG
Tsp45I 252 GTCAC ^GT[GC]AC
1029 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AcsI AluI ApoI BbvI BsaJI
BsiYI BslI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI EaeI EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI MaeIII MboII MnlI
MseI MspI MvaI NlaIII NlaIV
Sau96I ScrFI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I