Restriction map for
pF1KB8628
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AspEI AviII AvrII BanI
BbeI BbrPI BbvCI BcgI BclI
BfrI BglI BlnI BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstUI BstXI
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRV EcoT22I FbaI FseI FspI
HaeII Hin1I HpaI KasI KpnI
KspI MamI MluI MroI MspA1I
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SphI Sse8387I StuI XcmI
XhoI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AlwNI 2299 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2322 CCTAAGG CC^T[ACGT]AGG
AseI 2406 ATTAAT AT^TAAT
AsnI 2406 ATTAAT AT^TAAT
Asp700I 1168 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 70 GTGCTC G[AT]GC[AT]^C
AspI 897 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1645 CCCGGG C^[CT]CG[GA]G
BalI 1983 TGGCCA TGG^CCA
BamHI 831 GGATCC G^GATCC
BbsI 521 GTCTTC GAAGAC(2/6)
BlpI 2338 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2338 GCTCAGC GC^T[ACGT]AGC
BpuAI 521 GTCTTC GAAGAC(2/6)
BsgI 1635 CTGCAC GTGCAG(16/14)
BsiHKAI 70 GTGCTC G[AT]GC[AT]^C
BsoBI 1645 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2264 TGTACA T^GTACA
BsrGI 2264 TGTACA T^GTACA
Bst1107I 767 GTATAC GTA^TAC
BstZ17I 767 GTATAC GTA^TAC
Bsu36I 2322 CCTAAGG CC^T[ACGT]AGG
CelII 2338 GCTCAGC GC^T[ACGT]AGC
CfoI 1047 GCGC GCG^C
DraII 975 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 2041 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2322 CCTAAGG CC^T[ACGT]AGG
EcoO109I 975 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 415 GAATTC G^AATTC
FauI 1111 GCGGG CCCGC(4/6)
HapII 1646 CCGG C^CGG
HgaI 1807 GACGC GACGC(5/10)
HhaI 1047 GCGC GCG^C
HinP1I 1047 GCGC G^CGC
HincII 888 GTCAAC GT[CT]^[GA]AC
HindII 888 GTCAAC GT[CT]^[GA]AC
HpaII 1646 CCGG C^CGG
MluNI 1983 TGGCCA TGG^CCA
MscI 1983 TGGCCA TGG^CCA
MslI 238 CACACAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1646 CCGG C^CGG
MwoI 1976 GCATGTATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflFI 897 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 975 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1948 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2406 ATTAAT AT^TAAT
SapI 2383 GAAGAGC GCTCTTC(1/4)
SmaI 1645 CCCGGG CCC^GGG
SmlI 1540 CTTGAG C^T[CT][GA]AG
SpeI 1735 ACTAGT A^CTAGT
SspBI 2264 TGTACA T^GTACA
SwaI 1394 ATTTAAAT ATTT^AAAT
Tth111I 897 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 561 TCTAGA T^CTAGA
XmaCI 1645 CCCGGG C^CCGGG
XmaI 1645 CCCGGG C^CCGGG
XmnI 1168 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 767 GTATAC GT^[AC][GT]AC
776 GTAGAC
AvaII 976 GGACC G^G[AT]CC
2086 GGTCC
BanII 2049 GAGCCC G[GA]GC[CT]^C
2375 GAGCCC
BciVI 387 GGATAC GTATCC(6/5)
1592 GGATAC
BcnI 1645 CCCGG CC^[GC]GG
1646 CCGGG
BglII 564 AGATCT A^GATCT
1068 AGATCT
BsaI 1074 GAGACC GGTCTC(1/5)
1297 GGTCTC
BseRI 1079 CTCCTC GAGGAG(10/8)
1408 CTCCTC
BsmFI 1556 GGGAC GGGAC(10/14)
1836 GGGAC
BstEII 1258 GGTAACC G^GT[ACGT]ACC
1749 GGTCACC
BstPI 1258 GGTAACC G^GT[ACGT]ACC
1749 GGTCACC
DsaI 396 CCATGG C^C[GA][CT]GG
2054 CCATGG
EaeI 621 TGGCCG [CT]^GGCC[GA]
1983 TGGCCA
EcoO65I 1258 GGTAACC G^GT[ACGT]ACC
1749 GGTCACC
HindIII 207 AAGCTT A^AGCTT
1059 AAGCTT
MaeII 901 ACGT A^CGT
2418 ACGT
MfeI 105 CAATTG C^AATTG
666 CAATTG
MunI 105 CAATTG C^AATTG
666 CAATTG
NciI 1645 CCCGG CC^[GC]GG
1646 CCGGG
NcoI 396 CCATGG C^CATGG
2054 CCATGG
PflMI 396 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2077 CCATCCTTTGG
PleI 482 GACTC GAGTC(4/5)
519 GAGTC
PstI 1582 CTGCAG CTGCA^G
1874 CTGCAG
SexAI 930 ACCAGGT A^CC[AT]GGT
1571 ACCTGGT
TaqI 611 TCGA T^CGA
1655 TCGA
TthHB8I 611 TCGA T^CGA
1655 TCGA
Van91I 396 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2077 CCATCCTTTGG
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# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BfaI BmyI BpmI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MvaI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII