Restriction map for
pF1KB8625
##################################
# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AvaI
AviII BamHI BbeI BbrPI BbvCI
BcgI BclI BfrI BglII BsaAI
BsaHI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspLU11I BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I CfoI Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HincII
HindII HpaI KasI KpnI KspI
MaeII MfeI MflI MluI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RsaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccIII 960 TCCGGA T^CCGGA
AlwI 951 GGATC GGATC(4/5)
AlwNI 526 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 296 CCTCAGG CC^T[ACGT]AGG
ApaI 301 GGGCCC GGGCC^C
AspI 528 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 486 CCTAGG C^CTAGG
BanI 614 GGCACC G^G[CT][GA]CC
BbsI 201 GAAGAC GAAGAC(2/6)
BciVI 429 GGATAC GTATCC(6/5)
BglI 265 GCCAGACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 486 CCTAGG C^CTAGG
BlpI 52 GCTCAGC GC^T[ACGT]AGC
BpmI 733 CTGGAG CTGGAG(16/14)
Bpu1102I 52 GCTCAGC GC^T[ACGT]AGC
BpuAI 201 GAAGAC GAAGAC(2/6)
BsaBI 952 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 292 GAGACC GGTCTC(1/5)
BsaWI 960 TCCGGA [AT]^CCGG[AT]
BseAI 960 TCCGGA T^CCGGA
BsgI 73 GTGCAG GTGCAG(16/14)
BsmFI 553 GTCCC GGGAC(10/14)
BspEI 960 TCCGGA T^CCGGA
BspHI 381 TCATGA T^CATGA
BspMI 503 ACCTGC ACCTGC(4/8)
Bsu36I 296 CCTCAGG CC^T[ACGT]AGG
CelII 52 GCTCAGC GC^T[ACGT]AGC
DrdI 544 GACCTGGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 296 CCTCAGG CC^T[ACGT]AGG
EcoRI 816 GAATTC G^AATTC
HgaI 110 GACGC GACGC(5/10)
HindIII 157 AAGCTT A^AGCTT
HphI 720 GGTGA GGTGA(8/7)
MamI 952 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 960 TCCGGA T^CCGGA
MseI 806 TTAA T^TAA
MslI 591 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 35 GCATGT [GA]CATG^[CT]
PflFI 528 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 884 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 343 CTGCAG CTGCA^G
PvuII 190 CAGCTG CAG^CTG
RcaI 381 TCATGA T^CATGA
SfcI 343 CTGCAG C^T[GA][CT]AG
SfiI 264 GGCCAGACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tru9I 806 TTAA T^TAA
Tth111I 528 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 816 GAATTC [GA]^AATT[CT]
985 AAATTC
ApoI 816 GAATTC [GA]^AATT[CT]
985 AAATTC
Asp700I 87 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
939 GAAGAAGTTC
AspHI 661 GAGCTC G[AT]GC[AT]^C
690 GAGCTC
BalI 44 TGGCCA TGG^CCA
272 TGGCCA
BfaI 349 CTAG C^TAG
487 CTAG
BsiHKAI 661 GAGCTC G[AT]GC[AT]^C
690 GAGCTC
BsmAI 292 GAGAC GTCTC(1/5)
844 GAGAC
BsrI 270 ACTGG ACTGG(1/-1)
776 CCAGT
BssSI 61 CACGAG CACGAG(-5/-1)
693 CTCGTG
DraII 300 AGGGCCC [GA]G^G[ACGT]CC[CT]
301 GGGCCCC
DsaI 47 CCATGG C^C[GA][CT]GG
893 CCATGG
EarI 216 GAAGAG CTCTTC(1/4)
328 GAAGAG
EcoO109I 300 AGGGCCC [GA]G^G[ACGT]CC[CT]
301 GGGCCCC
Ksp632I 216 GAAGAG CTCTTC(1/4)
328 GAAGAG
MaeI 349 CTAG C^TAG
487 CTAG
MaeIII 721 GTGAC ^GT[ACGT]AC
779 GTGAC
MluNI 44 TGGCCA TGG^CCA
272 TGGCCA
MscI 44 TGGCCA TGG^CCA
272 TGGCCA
NcoI 47 CCATGG C^CATGG
893 CCATGG
PleI 412 GACTC GAGTC(4/5)
846 GACTC
SacI 661 GAGCTC GAGCT^C
690 GAGCTC
SfaNI 370 GATGC GCATC(5/9)
729 GCATC
SmlI 394 CTCAAG C^T[CT][GA]AG
631 CTCAAG
TaqI 29 TCGA T^CGA
108 TCGA
TfiI 378 GAATC G^A[AT]TC
933 GATTC
Tsp45I 721 GTGAC ^GT[GC]AC
779 GTGAC
TspRI 777 CAGTG CA[GC]TG[ACGT][ACGT]^
840 CAGTG
TthHB8I 29 TCGA T^CGA
108 TCGA
XmnI 87 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
939 GAAGAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BcnI BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BsrBI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
StyI TseI Tsp509I