Restriction map for
pF1KB8592
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiHKAI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstBI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraII
DraIII DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoRV FauI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HpaI HpaII KasI KpnI KspI
MamI MluI MroI MspA1I MspI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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BalI 65 TGGCCA TGG^CCA
BglII 1308 AGATCT A^GATCT
BmyI 2268 GTGCCC G[AGT]GC[ACT]^C
BpmI 371 CTCCAG CTGGAG(16/14)
BsaAI 2132 TACGTG [CT]AC^GT[GA]
BsaJI 261 CCAAGG C^C[ACGT][ACGT]GG
BsiWI 1146 CGTACG C^GTACG
BsmAI 1151 GAGAC GTCTC(1/5)
Bsp1286I 2268 GTGCCC G[AGT]GC[ACT]^C
BsrDI 23 CATTGC GCAATG(2/0)
BssKI 93 CCTGG ^CC[ACGT]GG
BssSI 179 CTCGTG CACGAG(-5/-1)
BstAPI 1942 GCAGATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 426 GGTGACC G^GT[ACGT]ACC
BstNI 93 CCTGG CC^[AT]GG
BstPI 426 GGTGACC G^GT[ACGT]ACC
BstXI 136 CCAAAAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1308 AGATCT [GA]^GATC[CT]
DrdI 2011 GACAATGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 65 TGGCCA [CT]^GGCC[GA]
EcoO65I 426 GGTGACC G^GT[ACGT]ACC
EcoRI 1815 GAATTC G^AATTC
EcoRII 93 CCTGG ^CC[AT]GG
EcoT14I 261 CCAAGG C^C[AT][AT]GG
EcoT22I 1916 ATGCAT ATGCA^T
MflI 1308 AGATCT [GA]^GATC[CT]
MluNI 65 TGGCCA TGG^CCA
MscI 65 TGGCCA TGG^CCA
MvaI 93 CCTGG CC^[AT]GG
NsiI 1916 ATGCAT ATGCA^T
SapI 379 GAAGAGC GCTCTTC(1/4)
ScaI 172 AGTACT AGT^ACT
ScrFI 93 CCTGG CC^[ACGT]GG
StuI 441 AGGCCT AGG^CCT
StyI 261 CCAAGG C^C[AT][AT]GG
XbaI 1779 TCTAGA T^CTAGA
XhoII 1308 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwI 2113 GATCC GGATC(4/5)
232 GGATC
Asp700I 663 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1081 GAACAGGTTC
AvaII 203 GGTCC G^G[AT]CC
900 GGTCC
BclI 2223 TGATCA T^GATCA
2343 TGATCA
BsiYI 1992 CCCCTCTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1993 CCCTCTTGGGG
BslI 1992 CCCCTCTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1993 CCCTCTTGGGG
BsmI 1363 GAATGC GAATGC(1/-1)
256 GCATTC
BspLU11I 416 ACATGT A^CATGT
2244 ACATGT
BspMI 1169 ACCTGC ACCTGC(4/8)
2310 GCAGGT
Cfr13I 203 GGTCC G^G[ACGT]CC
900 GGTCC
FbaI 2223 TGATCA T^GATCA
2343 TGATCA
HincII 1141 GTCAAC GT[CT]^[GA]AC
2123 GTCAAC
HindII 1141 GTCAAC GT[CT]^[GA]AC
2123 GTCAAC
HindIII 633 AAGCTT A^AGCTT
886 AAGCTT
MfeI 243 CAATTG C^AATTG
734 CAATTG
MunI 243 CAATTG C^AATTG
734 CAATTG
NlaIV 203 GGTCCC GG[ACGT]^[ACGT]CC
1373 GGCTCC
Sau96I 203 GGTCC G^G[ACGT]CC
900 GGTCC
TfiI 1446 GAATC G^A[AT]TC
2161 GATTC
XmnI 663 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1081 GAACAGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AcsI AfaI AflIII AluI ApoI
BbsI BbvI BfaI BpuAI BsgI
BsmFI BsrI BstF5I Cac8I DdeI
DpnII DraI EarI Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MwoI NdeII
NlaIII NspI PleI PstI RsaI
Sau3AI SfaNI SfcI SmlI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I