Restriction map for
pF1KB8591
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AvrII BbrPI BbvCI
BcgI BclI BfrI BlnI BsaAI
BsaBI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I ClaI CpoI DraI DrdI
EarI Eco47III EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MamI
MfeI MluI MroI MslI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
StuI SwaI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AflIII 2290 ACATGT A^C[GA][CT]GT
AocI 313 CCTGAGG CC^T[ACGT]AGG
ApaI 1964 GGGCCC GGGCC^C
AspI 1579 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1740 TGCGCA TGC^GCA
BamHI 210 GGATCC G^GATCC
BbeI 238 GGCGCC GGCGC^C
BbsI 840 GAAGAC GAAGAC(2/6)
BciVI 94 GTATCC GTATCC(6/5)
BglI 654 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1428 AGATCT A^GATCT
BpuAI 840 GAAGAC GAAGAC(2/6)
BsaI 285 GAGACC GGTCTC(1/5)
BsgI 1814 CTGCAC GTGCAG(16/14)
BsiEI 99 CGGCCG CG[GA][CT]^CG
BspLU11I 2290 ACATGT A^CATGT
BsrDI 390 CATTGC GCAATG(2/0)
BsrFI 3 GCCGGC [GA]^CCGG[CT]
Bsu36I 313 CCTGAGG CC^T[ACGT]AGG
Cfr10I 3 GCCGGC [GA]^CCGG[CT]
EagI 99 CGGCCG C^GGCCG
EclXI 99 CGGCCG C^GGCCG
Eco52I 99 CGGCCG C^GGCCG
Eco81I 313 CCTGAGG CC^T[ACGT]AGG
EcoNI 1569 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1865 CCATGG C^C[AT][AT]GG
FspI 1740 TGCGCA TGC^GCA
KasI 238 GGCGCC G^GCGCC
MseI 791 TTAA T^TAA
NaeI 3 GCCGGC GCC^GGC
NarI 238 GGCGCC GG^CGCC
NcoI 1865 CCATGG C^CATGG
NgoMIV 3 GCCGGC G^CCGGC
PflFI 1579 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 925 CTGCAG CTGCA^G
SacI 1406 GAGCTC GAGCT^C
SbfI 924 CCTGCAGG CCTGCA^GG
SfoI 238 GGCGCC GGC^GCC
Sse8387I 924 CCTGCAGG CCTGCA^GG
StyI 1865 CCATGG C^C[AT][AT]GG
TaqI 945 TCGA T^CGA
Tru9I 791 TTAA T^TAA
Tth111I 1579 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 945 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 238 GGCGCC G[GA]^CG[CT]C
2045 GGCGTC
AhdI 1103 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1654 GACATAGAGTC
AlwNI 533 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
893 CAGGGCCTG
AspEI 1103 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1654 GACATAGAGTC
BalI 1266 TGGCCA TGG^CCA
2189 TGGCCA
BfaI 2081 CTAG C^TAG
2284 CTAG
BsaHI 238 GGCGCC G[GA]^CG[CT]C
2045 GGCGTC
BsmBI 1543 CGTCTC CGTCTC(1/5)
728 GAGACG
BspMI 881 ACCTGC ACCTGC(4/8)
474 GCAGGT
BstEII 1323 GGTGACC G^GT[ACGT]ACC
1851 GGTGACC
BstPI 1323 GGTGACC G^GT[ACGT]ACC
1851 GGTGACC
BstYI 210 GGATCC [GA]^GATC[CT]
1428 AGATCT
DraIII 1318 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
1969 CACACAGTG
Eam1105I 1103 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1654 GACATAGAGTC
EcoO65I 1323 GGTGACC G^GT[ACGT]ACC
1851 GGTGACC
HaeII 238 GGCGCC [GA]GCGC^[CT]
1870 GGCGCT
HgaI 104 GCGTC GACGC(5/10)
2046 GCGTC
Hin1I 238 GGCGCC G[GA]^CG[CT]C
2045 GGCGTC
MaeI 2081 CTAG C^TAG
2284 CTAG
MaeII 865 ACGT A^CGT
1704 ACGT
MflI 210 GGATCC [GA]^GATC[CT]
1428 AGATCT
MluNI 1266 TGGCCA TGG^CCA
2189 TGGCCA
MscI 1266 TGGCCA TGG^CCA
2189 TGGCCA
NspI 1743 GCATGT [GA]CATG^[CT]
2290 ACATGT
PshAI 151 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
358 GACGAATGTC
SmlI 487 CTTGAG C^T[CT][GA]AG
696 CTTGAG
XhoII 210 GGATCC [GA]^GATC[CT]
1428 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BlpI BmyI BpmI
Bpu1102I BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI TfiI TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XmaCI XmaI