Restriction map for
pF1KB8590
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccII AciI
AclI AcyI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BbeI
BbrPI BbsI BbvCI BcgI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FauI FokI FseI
FspI HaeII HgaI Hin1I HindIII
KasI KpnI KspI MaeII MamI
MluI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 26 TCCGGA T^CCGGA
Alw44I 734 GTGCAC G^TGCAC
ApaLI 734 GTGCAC G^TGCAC
AspHI 734 GTGCAC G[AT]GC[AT]^C
AvaII 634 GGTCC G^G[AT]CC
BamHI 155 GGATCC G^GATCC
BanI 814 GGTGCC G^G[CT][GA]CC
BanII 552 GAGCCC G[GA]GC[CT]^C
BciVI 972 GGATAC GTATCC(6/5)
BclI 231 TGATCA T^GATCA
BsaI 757 GGTCTC GGTCTC(1/5)
BsaWI 26 TCCGGA [AT]^CCGG[AT]
BseAI 26 TCCGGA T^CCGGA
BseRI 614 CTCCTC GAGGAG(10/8)
BsiHKAI 734 GTGCAC G[AT]GC[AT]^C
BsmFI 303 GGGAC GGGAC(10/14)
BsmI 521 GAATGC GAATGC(1/-1)
BspDI 207 ATCGAT AT^CGAT
BspEI 26 TCCGGA T^CCGGA
BsrDI 835 GCAATG GCAATG(2/0)
CfoI 706 GCGC GCG^C
ClaI 207 ATCGAT AT^CGAT
DraI 223 TTTAAA TTT^AAA
DraII 633 GGGTCCT [GA]G^G[ACGT]CC[CT]
EarI 638 CTCTTC CTCTTC(1/4)
EcoO109I 633 GGGTCCT [GA]G^G[ACGT]CC[CT]
FbaI 231 TGATCA T^GATCA
HhaI 706 GCGC GCG^C
HinP1I 706 GCGC G^CGC
HincII 114 GTTAAC GT[CT]^[GA]AC
HindII 114 GTTAAC GT[CT]^[GA]AC
HinfI 102 GAATC G^A[ACGT]TC
HpaI 114 GTTAAC GTT^AAC
Ksp632I 638 CTCTTC CTCTTC(1/4)
MfeI 485 CAATTG C^AATTG
MroI 26 TCCGGA T^CCGGA
MspA1I 665 CAGCTG C[AC]G^C[GT]G
MunI 485 CAATTG C^AATTG
NcoI 506 CCATGG C^CATGG
NdeI 542 CATATG CA^TATG
NspI 863 GCATGT [GA]CATG^[CT]
PflMI 916 CCAATGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 633 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 665 CAGCTG CAG^CTG
SfaNI 954 GATGC GCATC(5/9)
SspI 624 AATATT AAT^ATT
TfiI 102 GAATC G^A[AT]TC
Van91I 916 CCAATGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 87 GAATTC [GA]^AATT[CT]
368 GAATTC
ApoI 87 GAATTC [GA]^AATT[CT]
368 GAATTC
Asp700I 368 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
715 GAAAAGTTTC
BalI 174 TGGCCA TGG^CCA
695 TGGCCA
BfaI 41 CTAG C^TAG
122 CTAG
BmyI 552 GAGCCC G[AGT]GC[ACT]^C
734 GTGCAC
BpmI 257 CTGGAG CTGGAG(16/14)
1016 CTGGAG
BsgI 602 GTGCAG GTGCAG(16/14)
902 GTGCAG
BsiYI 741 CCTGGGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
916 CCAATGTATGG
BslI 741 CCTGGGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
916 CCAATGTATGG
BsmAI 23 GTCTC GTCTC(1/5)
758 GTCTC
Bsp1286I 552 GAGCCC G[AGT]GC[ACT]^C
734 GTGCAC
BspHI 452 TCATGA T^CATGA
461 TCATGA
BstYI 155 GGATCC [GA]^GATC[CT]
781 GGATCT
DsaI 506 CCATGG C^C[GA][CT]GG
806 CCGTGG
EaeI 174 TGGCCA [CT]^GGCC[GA]
695 TGGCCA
EcoRI 87 GAATTC G^AATTC
368 GAATTC
EcoT14I 65 CCAAGG C^C[AT][AT]GG
506 CCATGG
HapII 27 CCGG C^CGG
159 CCGG
HpaII 27 CCGG C^CGG
159 CCGG
MaeI 41 CTAG C^TAG
122 CTAG
MaeIII 481 GTGAC ^GT[ACGT]AC
493 GTGAC
MflI 155 GGATCC [GA]^GATC[CT]
781 GGATCT
MluNI 174 TGGCCA TGG^CCA
695 TGGCCA
MscI 174 TGGCCA TGG^CCA
695 TGGCCA
MslI 378 CACCTATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
586 CATTCTGGTG
MspI 27 CCGG C^CGG
159 CCGG
RcaI 452 TCATGA T^CATGA
461 TCATGA
StuI 68 AGGCCT AGG^CCT
681 AGGCCT
StyI 65 CCAAGG C^C[AT][AT]GG
506 CCATGG
TaqI 208 TCGA T^CGA
952 TCGA
Tsp45I 481 GTGAC ^GT[GC]AC
493 GTGAC
TthHB8I 208 TCGA T^CGA
952 TCGA
XhoII 155 GGATCC [GA]^GATC[CT]
781 GGATCT
XmnI 368 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
715 GAAAAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AfaI AluI AlwI BbvI BsaJI
BsrI BssKI BstNI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI HaeIII
HphI ItaI MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
Tru9I TseI Tsp509I TspRI