Restriction map for
pF1KB8587
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AlwI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BclI
BcnI BfrI BglII BlnI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmAI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HapII Hin1I HincII HindII HindIII
HpaI HpaII HphI KasI KpnI
KspI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MslI
MspI MunI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 79 CGCG CG^CG
AhdI 271 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 617 AGCT AG^CT
AlwNI 528 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 65 GGGCCC GGGCC^C
AspEI 271 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 507 GAGCAC G[AT]GC[AT]^C
AvaI 68 CCCGAG C^[CT]CG[GA]G
BbsI 699 GAAGAC GAAGAC(2/6)
BciVI 537 GTATCC GTATCC(6/5)
BfaI 4 CTAG C^TAG
BglI 94 GCCATGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 118 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 118 GCTCAGC GC^T[ACGT]AGC
BpuAI 699 GAAGAC GAAGAC(2/6)
BseRI 395 CTCCTC GAGGAG(10/8)
BsgI 525 GTGCAG GTGCAG(16/14)
BsiHKAI 507 GAGCAC G[AT]GC[AT]^C
BsoBI 68 CCCGAG C^[CT]CG[GA]G
BsrDI 347 CATTGC GCAATG(2/0)
BsrI 468 CCAGT ACTGG(1/-1)
BstF5I 125 GGATG GGATG(2/0)
BstUI 79 CGCG CG^CG
Cac8I 354 GCAGGC GC[ACGT]^[ACGT]GC
CelII 118 GCTCAGC GC^T[ACGT]AGC
CfoI 89 GCGC GCG^C
DdeI 119 CTCAG C^T[ACGT]AG
DpnII 211 GATC ^GATC
DsaI 95 CCATGG C^C[GA][CT]GG
Eam1105I 271 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 666 GAAGAG CTCTTC(1/4)
EcoNI 315 CCTTACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 33 GCGGG CCCGC(4/6)
FokI 125 GGATG GGATG(9/13)
HgaI 530 GACGC GACGC(5/10)
HhaI 89 GCGC GCG^C
HinP1I 89 GCGC G^CGC
Ksp632I 666 GAAGAG CTCTTC(1/4)
MaeI 4 CTAG C^TAG
MaeIII 50 GTCAC ^GT[ACGT]AC
MboI 211 GATC ^GATC
MseI 515 TTAA T^TAA
MspA1I 121 CAGCGG C[AC]G^C[GT]G
MvnI 79 CGCG CG^CG
NcoI 95 CCATGG C^CATGG
NdeII 211 GATC ^GATC
PpuMI 231 AGGTCCT [GA]G^G[AT]CC[CT]
Sau3AI 211 GATC ^GATC
Tru9I 515 TTAA T^TAA
Tsp45I 50 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 13 enzymes) #
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AfaI 338 GTAC GT^AC
382 GTAC
BsiYI 315 CCTTACCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
412 CCACTTGCAGG
BslI 315 CCTTACCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
412 CCACTTGCAGG
BspHI 432 TCATGA T^CATGA
473 TCATGA
DraII 64 GGGGCCC [GA]G^G[ACGT]CC[CT]
231 AGGTCCT
EcoO109I 64 GGGGCCC [GA]G^G[ACGT]CC[CT]
231 AGGTCCT
EcoT14I 95 CCATGG C^C[AT][AT]GG
320 CCAAGG
NspI 275 GCATGT [GA]CATG^[CT]
461 ACATGC
RcaI 432 TCATGA T^CATGA
473 TCATGA
RsaI 338 GTAC GT^AC
382 GTAC
SfaNI 297 GCATC GCATC(5/9)
608 GCATC
StyI 95 CCATGG C^C[AT][AT]GG
320 CCAAGG
TspRI 326 CAGTG CA[GC]TG[ACGT][ACGT]^
510 CACTG
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AcsI ApoI AvaII BanII
BbvI BmyI BpmI BsaJI Bsp1286I
BssKI BstNI Cfr13I EcoRII Fnu4HI
HaeIII HinfI ItaI MboII MnlI
MvaI MwoI NlaIII NlaIV PleI
Sau96I ScrFI TseI Tsp509I