Restriction map for
pF1KB8576
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# NO CUT ENZYMES ( 195 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AfaI AflII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspHI AspI AvaI AvaII AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbvCI BbvI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FauI FbaI Fnu4HI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII HphI ItaI
KasI KpnI KspI MaeII MamI
MfeI MluI MroI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TseI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccI 520 GTAGAC GT^[AC][GT]AC
AflIII 206 ACATGT A^C[GA][CT]GT
AhdI 17 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 264 AGCT AG^CT
AlwNI 433 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 17 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 311 TGGCCA TGG^CCA
BbsI 552 GAAGAC GAAGAC(2/6)
BpuAI 552 GAAGAC GAAGAC(2/6)
BsiYI 145 CCAACAATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 145 CCAACAATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 429 GTCTC GTCTC(1/5)
BspLU11I 206 ACATGT A^CATGT
BssKI 467 CCTGG ^CC[ACGT]GG
BstNI 467 CCTGG CC^[AT]GG
Cac8I 506 GCTTGC GC[ACGT]^[ACGT]GC
EaeI 311 TGGCCA [CT]^GGCC[GA]
Eam1105I 17 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 332 CTCTTC CTCTTC(1/4)
EcoRI 365 GAATTC G^AATTC
EcoRII 467 CCTGG ^CC[AT]GG
EcoT14I 66 CCTTGG C^C[AT][AT]GG
Ksp632I 332 CTCTTC CTCTTC(1/4)
MaeIII 412 GTGAC ^GT[ACGT]AC
MluNI 311 TGGCCA TGG^CCA
MnlI 49 GAGG CCTC(7/6)
MscI 311 TGGCCA TGG^CCA
MslI 472 CATATTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 467 CCTGG CC^[AT]GG
MwoI 481 GCTAGTGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 206 ACATGT [GA]CATG^[CT]
ScrFI 467 CCTGG CC^[ACGT]GG
StyI 66 CCTTGG C^C[AT][AT]GG
Tsp45I 412 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AcsI 169 AAATTC [GA]^AATT[CT]
365 GAATTC
AlwI 540 GATCC GGATC(4/5)
396 GGATC
ApoI 169 AAATTC [GA]^AATT[CT]
365 GAATTC
BsaJI 66 CCTTGG C^C[ACGT][ACGT]GG
466 CCCTGG
BsrI 524 ACTGG ACTGG(1/-1)
182 CCAGT
BstF5I 179 CATCC GGATG(2/0)
343 CATCC
BstYI 396 GGATCT [GA]^GATC[CT]
539 AGATCC
DdeI 400 CTTAG C^T[ACGT]AG
431 CTCAG
FokI 179 CATCC GGATG(9/13)
343 CATCC
HinfI 155 GATTC G^A[ACGT]TC
339 GAATC
MflI 396 GGATCT [GA]^GATC[CT]
539 AGATCC
NlaIII 55 CATG CATG^
207 CATG
SfaNI 406 GATGC GCATC(5/9)
487 GATGC
TaqI 326 TCGA T^CGA
363 TCGA
TfiI 155 GATTC G^A[AT]TC
339 GAATC
Tsp509I 170 AATT ^AATT
366 AATT
TspRI 197 CAGTG CA[GC]TG[ACGT][ACGT]^
410 CAGTG
TthHB8I 326 TCGA T^CGA
363 TCGA
XhoII 396 GGATCT [GA]^GATC[CT]
539 AGATCC
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
#########################################
BfaI DpnII HaeIII MaeI MboI
MboII MseI NdeII Sau3AI Tru9I