Restriction map for
pF1KB8447
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII AccI AccIII AclI AflII
AgeI AhdI AlwI Aor51HI AscI
AseI AsnI Asp700I AspEI AvaI
AviII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlpI BpmI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsmI BsoBI Bsp1407I BspEI BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstXI BstYI BstZ17I CelII
CfoI CpoI DpnII DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoT22I FbaI FseI FspI
HaeII HhaI HinP1I KasI MamI
MboI MfeI MflI MluI MluNI
MroI MscI MunI NarI NdeII
NheI NotI NruI NsiI PacI
PaeR7I PinAI PleI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
RsrII SalI SapI Sau3AI SbfI
ScaI SexAI SfcI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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Acc65I 969 GGTACC G^GTACC
AccII 1036 CGCG CG^CG
Alw44I 10 GTGCAC G^TGCAC
AlwNI 847 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1293 CCTCAGG CC^T[ACGT]AGG
ApaI 1227 GGGCCC GGGCC^C
ApaLI 10 GTGCAC G^TGCAC
Asp718I 969 GGTACC G^GTACC
AspI 720 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1083 CCTAGG C^CTAGG
BanI 969 GGTACC G^G[CT][GA]CC
BbsI 660 GAAGAC GAAGAC(2/6)
BbvI 707 GCTGC GCAGC(8/12)
BfaI 1084 CTAG C^TAG
BglI 1148 GCCAGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1083 CCTAGG C^CTAGG
BpuAI 660 GAAGAC GAAGAC(2/6)
BseRI 375 GAGGAG GAGGAG(10/8)
BsiWI 1274 CGTACG C^GTACG
BsmBI 616 GAGACG CGTCTC(1/5)
BspDI 790 ATCGAT AT^CGAT
BspHI 249 TCATGA T^CATGA
BsrDI 996 CATTGC GCAATG(2/0)
BsrFI 1105 GCCGGC [GA]^CCGG[CT]
BstEII 474 GGTGACC G^GT[ACGT]ACC
BstPI 474 GGTGACC G^GT[ACGT]ACC
BstUI 1036 CGCG CG^CG
Bsu36I 1293 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1105 GCCGGC [GA]^CCGG[CT]
ClaI 790 ATCGAT AT^CGAT
EaeI 1141 CGGCCA [CT]^GGCC[GA]
EarI 32 CTCTTC CTCTTC(1/4)
Eco81I 1293 CCTCAGG CC^T[ACGT]AGG
EcoNI 225 CCTAATTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 474 GGTGACC G^GT[ACGT]ACC
EcoRI 801 GAATTC G^AATTC
EcoRV 181 GATATC GAT^ATC
HincII 841 GTTAAC GT[CT]^[GA]AC
HindII 841 GTTAAC GT[CT]^[GA]AC
HindIII 442 AAGCTT A^AGCTT
HpaI 841 GTTAAC GTT^AAC
KpnI 969 GGTACC GGTAC^C
Ksp632I 32 CTCTTC CTCTTC(1/4)
KspI 1035 CCGCGG CCGC^GG
MaeI 1084 CTAG C^TAG
MaeII 350 ACGT A^CGT
MslI 151 CATTTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1036 CGCG CG^CG
NaeI 1105 GCCGGC GCC^GGC
NdeI 654 CATATG CA^TATG
NgoMIV 1105 GCCGGC G^CCGGC
PflFI 720 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1020 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 310 GACACATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 635 CAGCTG CAG^CTG
RcaI 249 TCATGA T^CATGA
SacI 603 GAGCTC GAGCT^C
SacII 1035 CCGCGG CCGC^GG
SphI 1046 GCATGC GCATG^C
TseI 707 GCTGC G^C[AT]GC
Tsp45I 475 GTGAC ^GT[GC]AC
Tth111I 720 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcyI 723 GGCGTC G[GA]^CG[CT]C
1375 GGCGTC
AflIII 144 ACATGT A^C[GA][CT]GT
313 ACATGT
AspHI 10 GTGCAC G[AT]GC[AT]^C
603 GAGCTC
AvaII 46 GGACC G^G[AT]CC
1021 GGACC
BanII 603 GAGCTC G[GA]GC[CT]^C
1227 GGGCCC
BsaHI 723 GGCGTC G[GA]^CG[CT]C
1375 GGCGTC
BsgI 1114 CTGCAC GTGCAG(16/14)
464 GTGCAG
BsiHKAI 10 GTGCAC G[AT]GC[AT]^C
603 GAGCTC
BsmAI 616 GAGAC GTCTC(1/5)
1076 GAGAC
BspLU11I 144 ACATGT A^CATGT
313 ACATGT
BstBI 550 TTCGAA TT^CGAA
737 TTCGAA
FauI 612 GCGGG CCCGC(4/6)
869 GCGGG
Fnu4HI 707 GCTGC GC^[ACGT]GC
1109 GCGGC
Hin1I 723 GGCGTC G[GA]^CG[CT]C
1375 GGCGTC
ItaI 707 GCTGC GC^[ACGT]GC
1109 GCGGC
NspV 550 TTCGAA TT^CGAA
737 TTCGAA
PflMI 37 CCATCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
846 CCAGGAACTGG
SfuI 550 TTCGAA TT^CGAA
737 TTCGAA
SmlI 439 CTCAAG C^T[CT][GA]AG
714 CTCAAG
Van91I 37 CCATCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
846 CCAGGAACTGG
XcmI 1149 CCAGGGTGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1380 CCAGTTTGGAGCTGG
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BcnI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DraII
DsaI EcoO109I EcoRII EcoT14I FokI
HaeIII HapII HgaI HinfI HpaII
HphI MaeIII MboII MnlI MseI
MspA1I MspI MvaI MwoI NciI
NcoI NlaIII NlaIV NspI RsaI
Sau96I ScrFI SfaNI StyI TaqI
TfiI Tru9I Tsp509I TspRI TthHB8I