Restriction map for
pF1KB8445
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp718I AviII AvrII BalI
BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BsaBI BsaHI BsaI
BsaWI BseAI BsiWI BsmBI BsmI
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI EagI EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HinfI
HpaI KasI KpnI KspI MaeI
MamI MfeI MflI MluI MluNI
MroI MscI MseI MunI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PflMI
PinAI PleI PmeI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI StuI StyI
SwaI TfiI Tru9I Van91I XbaI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 448 GTCTAC GT^[AC][GT]AC
AhdI 484 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 385 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 72 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 484 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 34 GTGCTC G[AT]GC[AT]^C
AspI 319 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 106 CTCGAG C^[CT]CG[GA]G
BbrPI 868 CACGTG CAC^GTG
BbsI 898 GTCTTC GAAGAC(2/6)
BlpI 12 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 12 GCTCAGC GC^T[ACGT]AGC
BpuAI 898 GTCTTC GAAGAC(2/6)
BsaAI 868 CACGTG [CT]AC^GT[GA]
BsgI 689 CTGCAC GTGCAG(16/14)
BsiEI 144 CGACCG CG[GA][CT]^CG
BsiHKAI 34 GTGCTC G[AT]GC[AT]^C
BsoBI 106 CTCGAG C^[CT]CG[GA]G
Bsp1407I 56 TGTACA T^GTACA
BsrGI 56 TGTACA T^GTACA
CelII 12 GCTCAGC GC^T[ACGT]AGC
DrdI 510 GACAGTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 881 CCACGG C^C[GA][CT]GG
Eam1105I 484 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 37 CTCTTC CTCTTC(1/4)
Ksp632I 37 CTCTTC CTCTTC(1/4)
MaeIII 195 GTCAC ^GT[ACGT]AC
MslI 552 CACCCCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 28 CATATG CA^TATG
NspI 398 GCATGC [GA]CATG^[CT]
PaeR7I 106 CTCGAG C^TCGAG
PflFI 319 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 868 CACGTG CAC^GTG
PmlI 868 CACGTG CAC^GTG
PpuMI 587 AGGACCC [GA]G^G[AT]CC[CT]
PstI 562 CTGCAG CTGCA^G
SapI 36 GCTCTTC GCTCTTC(1/4)
SfcI 562 CTGCAG C^T[GA][CT]AG
SmlI 106 CTCGAG C^T[CT][GA]AG
SphI 398 GCATGC GCATG^C
SspBI 56 TGTACA T^GTACA
Tsp45I 195 GTCAC ^GT[GC]AC
Tsp509I 252 AATT ^AATT
Tth111I 319 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 434 CCAGTGGGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 106 CTCGAG C^TCGAG
XmnI 72 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccII 534 CGCG CG^CG
574 CGCG
Aor51HI 83 AGCGCT AGC^GCT
887 AGCGCT
ApaI 5 GGGCCC GGGCC^C
429 GGGCCC
BsmAI 410 GAGAC GTCTC(1/5)
381 GTCTC
BspHI 68 TCATGA T^CATGA
581 TCATGA
BspMI 614 ACCTGC ACCTGC(4/8)
564 GCAGGT
BstUI 534 CGCG CG^CG
574 CGCG
DraIII 601 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
603 CACTGTGTG
EaeI 225 CGGCCA [CT]^GGCC[GA]
744 CGGCCA
Eco47III 83 AGCGCT AGC^GCT
887 AGCGCT
MaeII 19 ACGT A^CGT
869 ACGT
MvnI 534 CGCG CG^CG
574 CGCG
RcaI 68 TCATGA T^CATGA
581 TCATGA
SexAI 263 ACCTGGT A^CC[AT]GGT
320 ACCTGGT
SfaNI 558 GATGC GCATC(5/9)
548 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MboI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI TaqI
TseI TspRI TthHB8I