Restriction map for
pF1KB8413
##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI Alw44I Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AviII AvrII BamHI BanI BbeI
BbrPI BciVI BfrI BglII BlnI
BsaAI BsaBI BsaHI BsiEI BsiWI
BsmBI BspDI BspHI BsrBI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MluI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI SspI StuI SwaI XbaI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 1166 TCCGGA T^CCGGA
AcsI 1799 AAATTC [GA]^AATT[CT]
AocI 739 CCTGAGG CC^T[ACGT]AGG
ApoI 1799 AAATTC [GA]^AATT[CT]
AseI 715 ATTAAT AT^TAAT
AsnI 715 ATTAAT AT^TAAT
BbsI 1195 GAAGAC GAAGAC(2/6)
BcgI 1437 GCAAATCACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1619 TGATCA T^GATCA
BglI 1211 GCCCCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2046 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2046 GCTGAGC GC^T[ACGT]AGC
BpuAI 1195 GAAGAC GAAGAC(2/6)
BseAI 1166 TCCGGA T^CCGGA
BsgI 251 CTGCAC GTGCAG(16/14)
BspEI 1166 TCCGGA T^CCGGA
BspLU11I 2006 ACATGT A^CATGT
BspMI 1263 GCAGGT ACCTGC(4/8)
BssSI 394 CTCGTG CACGAG(-5/-1)
BstXI 1466 CCAACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 739 CCTGAGG CC^T[ACGT]AGG
CelII 2046 GCTGAGC GC^T[ACGT]AGC
EarI 1336 GAAGAG CTCTTC(1/4)
Eco81I 739 CCTGAGG CC^T[ACGT]AGG
EcoT22I 767 ATGCAT ATGCA^T
FauI 1569 CCCGC CCCGC(4/6)
FbaI 1619 TGATCA T^GATCA
HaeII 1631 AGCGCC [GA]GCGC^[CT]
Ksp632I 1336 GAAGAG CTCTTC(1/4)
MfeI 1574 CAATTG C^AATTG
MroI 1166 TCCGGA T^CCGGA
MunI 1574 CAATTG C^AATTG
NsiI 767 ATGCAT ATGCA^T
PaeR7I 1445 CTCGAG C^TCGAG
PshBI 715 ATTAAT AT^TAAT
SapI 1336 GAAGAGC GCTCTTC(1/4)
ScaI 1961 AGTACT AGT^ACT
SmaI 245 CCCGGG CCC^GGG
XhoI 1445 CTCGAG C^TCGAG
XmaCI 245 CCCGGG C^CCGGG
XmaI 245 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 557 ACGTGT A^C[GA][CT]GT
2006 ACATGT
AhdI 973 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1558 GACTTCCAGTC
AspEI 973 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1558 GACTTCCAGTC
BbvCI 169 CCTCAGC CCTCAGC(-5/-2)
1584 GCTGAGG
BfaI 463 CTAG C^TAG
989 CTAG
BsaWI 788 TCCGGT [AT]^CCGG[AT]
1166 TCCGGA
BseRI 1100 CTCCTC GAGGAG(10/8)
957 GAGGAG
Bsp1407I 176 TGTACA T^GTACA
1031 TGTACA
BsrFI 1640 ACCGGC [GA]^CCGG[CT]
1709 GCCGGT
BsrGI 176 TGTACA T^GTACA
1031 TGTACA
BstYI 1595 AGATCC [GA]^GATC[CT]
1607 AGATCC
CfoI 1530 GCGC GCG^C
1632 GCGC
Cfr10I 1640 ACCGGC [GA]^CCGG[CT]
1709 GCCGGT
Eam1105I 973 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1558 GACTTCCAGTC
HgaI 973 GACGC GACGC(5/10)
1289 GACGC
HhaI 1530 GCGC GCG^C
1632 GCGC
HinP1I 1530 GCGC G^CGC
1632 GCGC
MaeI 463 CTAG C^TAG
989 CTAG
MaeII 338 ACGT A^CGT
557 ACGT
MflI 1595 AGATCC [GA]^GATC[CT]
1607 AGATCC
NspI 1127 ACATGC [GA]CATG^[CT]
2006 ACATGT
PstI 1261 CTGCAG CTGCA^G
1789 CTGCAG
SbfI 1260 CCTGCAGG CCTGCA^GG
1788 CCTGCAGG
SmlI 1445 CTCGAG C^T[CT][GA]AG
1612 CTTGAG
Sse8387I 1260 CCTGCAGG CCTGCA^GG
1788 CCTGCAGG
SspBI 176 TGTACA T^GTACA
1031 TGTACA
TaqI 71 TCGA T^CGA
1446 TCGA
TthHB8I 71 TCGA T^CGA
1446 TCGA
XhoII 1595 AGATCC [GA]^GATC[CT]
1607 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AspI AvaI AvaII BalI
BanII BbvI BcnI BmyI BpmI
BsaI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflFI PflMI PleI PpuMI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
Van91I XcmI