Restriction map for
pF1KB8408
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII Acc65I AccI AccII AciI
AclI AcyI AgeI AhdI AlwNI
Aor51HI ApaI AscI Asp718I AspEI
AspI AvaI AviII BanI BbeI
BbsI BcgI BclI BcnI BglI
BpuAI BsaBI BsaHI BseRI BsiEI
BsiWI BsmBI BsmFI BsoBI BspDI
BspHI BspLU11I BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I CfoI
Cfr10I ClaI CpoI DraII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoRI EcoRV
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I KasI
KpnI Ksp632I KspI MamI MfeI
MluI MunI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccIII 745 TCCGGA T^CCGGA
AflII 1536 CTTAAG C^TTAAG
Alw44I 1050 GTGCAC G^TGCAC
AocI 116 CCTCAGG CC^T[ACGT]AGG
ApaLI 1050 GTGCAC G^TGCAC
AspHI 1050 GTGCAC G[AT]GC[AT]^C
BalI 1468 TGGCCA TGG^CCA
BamHI 1000 GGATCC G^GATCC
BanII 1593 GGGCTC G[GA]GC[CT]^C
BbrPI 1164 CACGTG CAC^GTG
BbvCI 179 CCTCAGC CCTCAGC(-5/-2)
BciVI 650 GGATAC GTATCC(6/5)
BfrI 1536 CTTAAG C^TTAAG
BglII 864 AGATCT A^GATCT
BlpI 1595 GCTCAGC GC^T[ACGT]AGC
BpmI 764 CTGGAG CTGGAG(16/14)
Bpu1102I 1595 GCTCAGC GC^T[ACGT]AGC
BsaI 400 GAGACC GGTCTC(1/5)
BsaWI 745 TCCGGA [AT]^CCGG[AT]
BseAI 745 TCCGGA T^CCGGA
BsgI 136 CTGCAC GTGCAG(16/14)
BsiHKAI 1050 GTGCAC G[AT]GC[AT]^C
BsmI 1292 GCATTC GAATGC(1/-1)
BspEI 745 TCCGGA T^CCGGA
BspMI 483 ACCTGC ACCTGC(4/8)
BstAPI 1182 GCATGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 116 CCTCAGG CC^T[ACGT]AGG
CelII 1595 GCTCAGC GC^T[ACGT]AGC
DraIII 1484 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1210 CCATGG C^C[GA][CT]GG
EaeI 1468 TGGCCA [CT]^GGCC[GA]
Eco81I 116 CCTCAGG CC^T[ACGT]AGG
EcoT22I 919 ATGCAT ATGCA^T
HapII 746 CCGG C^CGG
HincII 1194 GTTAAC GT[CT]^[GA]AC
HindII 1194 GTTAAC GT[CT]^[GA]AC
HpaI 1194 GTTAAC GTT^AAC
HpaII 746 CCGG C^CGG
MluNI 1468 TGGCCA TGG^CCA
MroI 745 TCCGGA T^CCGGA
MscI 1468 TGGCCA TGG^CCA
MspI 746 CCGG C^CGG
NcoI 1210 CCATGG C^CATGG
NdeI 1688 CATATG CA^TATG
NsiI 919 ATGCAT ATGCA^T
PmaCI 1164 CACGTG CAC^GTG
PmlI 1164 CACGTG CAC^GTG
ScaI 436 AGTACT AGT^ACT
TaqI 414 TCGA T^CGA
TthHB8I 414 TCGA T^CGA
XcmI 1667 CCAATTTGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcsI 1060 AAATTT [GA]^AATT[CT]
1400 AAATTC
ApoI 1060 AAATTT [GA]^AATT[CT]
1400 AAATTC
AseI 678 ATTAAT AT^TAAT
1332 ATTAAT
AsnI 678 ATTAAT AT^TAAT
1332 ATTAAT
Asp700I 310 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1288 GAAAGCATTC
AvaII 68 GGACC G^G[AT]CC
819 GGACC
AvrII 834 CCTAGG C^CTAGG
1005 CCTAGG
BlnI 834 CCTAGG C^CTAGG
1005 CCTAGG
Bsp1407I 163 TGTACA T^GTACA
733 TGTACA
BsrGI 163 TGTACA T^GTACA
733 TGTACA
Cac8I 1115 GCTTGC GC[ACGT]^[ACGT]GC
1600 GCCAGC
DraI 219 TTTAAA TTT^AAA
609 TTTAAA
EcoNI 116 CCTCAGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
607 CCTTTAAAAGG
HindIII 258 AAGCTT A^AGCTT
1633 AAGCTT
MboII 1516 GAAGA GAAGA(8/7)
1699 GAAGA
MspA1I 133 CAGCTG C[AC]G^C[GT]G
823 CAGCTG
NspI 516 GCATGT [GA]CATG^[CT]
1182 GCATGT
PshBI 678 ATTAAT AT^TAAT
1332 ATTAAT
PstI 1110 CTGCAG CTGCA^G
1189 CTGCAG
PvuII 133 CAGCTG CAG^CTG
823 CAGCTG
SfaNI 380 GCATC GCATC(5/9)
921 GCATC
SspBI 163 TGTACA T^GTACA
733 TGTACA
XmnI 310 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1288 GAAAGCATTC
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AfaI AflIII AluI AlwI BbvI
BfaI BmyI BsaAI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeII MaeIII MboI MflI MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII