Restriction map for
pF1KB8405
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI Asp718I AspEI AspI
AvaI AviII AvrII BbeI BbrPI
BbvCI BcgI BfrI BglII BlnI
BsaAI BsaWI BseAI BsiWI BsoBI
Bsp1407I BspDI BspEI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DrdI Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoT14I FseI
FspI KasI KpnI MfeI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacI
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StyI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 1747 GTCTAC GT^[AC][GT]AC
AcsI 1569 GAATTT [GA]^AATT[CT]
AcyI 1756 GACGCC G[GA]^CG[CT]C
AflIII 114 ACATGT A^C[GA][CT]GT
ApoI 1569 GAATTT [GA]^AATT[CT]
AseI 760 ATTAAT AT^TAAT
AsnI 760 ATTAAT AT^TAAT
AspHI 2259 GAGCAC G[AT]GC[AT]^C
BamHI 1309 GGATCC G^GATCC
BanI 1723 GGTGCC G^G[CT][GA]CC
BsaBI 2124 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1756 GACGCC G[GA]^CG[CT]C
BsiHKAI 2259 GAGCAC G[AT]GC[AT]^C
BsmBI 1208 CGTCTC CGTCTC(1/5)
BspHI 809 TCATGA T^CATGA
BspLU11I 114 ACATGT A^CATGT
BssSI 1742 CACGAG CACGAG(-5/-1)
DraIII 1484 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1761 CCGCGG C^C[GA][CT]GG
EagI 1764 CGGCCG C^GGCCG
EclXI 1764 CGGCCG C^GGCCG
Eco52I 1764 CGGCCG C^GGCCG
EcoNI 313 CCTGACGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1363 GATATC GAT^ATC
HaeII 371 AGCGCC [GA]GCGC^[CT]
Hin1I 1756 GACGCC G[GA]^CG[CT]C
HpaI 1584 GTTAAC GTT^AAC
KspI 1761 CCGCGG CCGC^GG
MaeII 1207 ACGT A^CGT
MamI 2124 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 1014 CATGCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PshBI 760 ATTAAT AT^TAAT
RcaI 809 TCATGA T^CATGA
SacII 1761 CCGCGG CCGC^GG
SapI 1942 GAAGAGC GCTCTTC(1/4)
SexAI 903 ACCAGGT A^CC[AT]GGT
SphI 1013 GCATGC GCATG^C
StuI 1850 AGGCCT AGG^CCT
TaqI 1631 TCGA T^CGA
TthHB8I 1631 TCGA T^CGA
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
ApaI 1576 GGGCCC GGGCC^C
1617 GGGCCC
BalI 1007 TGGCCA TGG^CCA
1711 TGGCCA
BbsI 2274 GAAGAC GAAGAC(2/6)
12 GTCTTC
BciVI 1407 GGATAC GTATCC(6/5)
2154 GTATCC
BglI 1726 GCCACTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2389 GCCCCGGAGGC
BlpI 1000 GCTCAGC GC^T[ACGT]AGC
1956 GCTAAGC
BpmI 982 CTCCAG CTGGAG(16/14)
1028 CTGGAG
Bpu1102I 1000 GCTCAGC GC^T[ACGT]AGC
1956 GCTAAGC
BpuAI 2274 GAAGAC GAAGAC(2/6)
12 GTCTTC
BsaI 1077 GGTCTC GGTCTC(1/5)
2072 GGTCTC
BseRI 169 GAGGAG GAGGAG(10/8)
1833 GAGGAG
BsgI 136 CTGCAC GTGCAG(16/14)
44 GTGCAG
BsiEI 1764 CGGCCG CG[GA][CT]^CG
2005 CGACCG
BsmI 1803 GAATGC GAATGC(1/-1)
1807 GCATTC
BspMI 2017 ACCTGC ACCTGC(4/8)
1017 GCAGGT
BsrBI 805 CCGCTC CCGCTC(-3/-3)
998 CCGCTC
BsrDI 64 GCAATG GCAATG(2/0)
1669 GCAATG
CelII 1000 GCTCAGC GC^T[ACGT]AGC
1956 GCTAAGC
DraII 1575 GGGGCCC [GA]G^G[ACGT]CC[CT]
1576 GGGCCCC
EcoO109I 1575 GGGGCCC [GA]G^G[ACGT]CC[CT]
1576 GGGCCCC
EcoT22I 1805 ATGCAT ATGCA^T
1864 ATGCAT
FauI 1760 CCCGC CCCGC(4/6)
1461 GCGGG
HgaI 1756 GACGC GACGC(5/10)
658 GCGTC
HindIII 421 AAGCTT A^AGCTT
2038 AAGCTT
MluNI 1007 TGGCCA TGG^CCA
1711 TGGCCA
MscI 1007 TGGCCA TGG^CCA
1711 TGGCCA
NsiI 1805 ATGCAT ATGCA^T
1864 ATGCAT
XcmI 1511 CCATAAATAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2256 CCAGAGCACTTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI Asp700I AvaII BanII BbvI
BclI BcnI BfaI BmyI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EarI EcoRII
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII XmnI