Restriction map for
pF1KB8404
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# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AcsI AflII AgeI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I AviII AvrII BbeI
BfrI BglII BlnI BsiWI BsmBI
Bsp1407I BspDI BsrGI BssHII Bst1107I
BstBI BstZ17I ClaI DraI Eco47III
EcoRI EcoRV FseI FspI HincII
HindII HindIII HpaI KasI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshBI PvuI SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tsp509I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Acc65I 652 GGTACC G^GTACC
AclI 2533 AACGTT AA^CGTT
AhdI 1548 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 652 GGTACC G^GTACC
AspEI 1548 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2834 GGATCC G^GATCC
BciVI 1143 GTATCC GTATCC(6/5)
BlpI 1789 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1789 GCTGAGC GC^T[ACGT]AGC
BsiEI 51 CGGCCG CG[GA][CT]^CG
BsmI 2148 GCATTC GAATGC(1/-1)
BspHI 1310 TCATGA T^CATGA
BspLU11I 1408 ACATGT A^CATGT
BsrBI 113 CCGCTC CCGCTC(-3/-3)
BstAPI 3125 GCAGCGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1789 GCTGAGC GC^T[ACGT]AGC
CpoI 2731 CGGTCCG CG^G[AT]CCG
DraIII 84 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 51 CGGCCG C^GGCCG
Eam1105I 1548 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 51 CGGCCG C^GGCCG
Eco52I 51 CGGCCG C^GGCCG
KpnI 652 GGTACC GGTAC^C
KspI 2621 CCGCGG CCGC^GG
NaeI 49 GCCGGC GCC^GGC
NcoI 2858 CCATGG C^CATGG
NgoMIV 49 GCCGGC G^CCGGC
PflMI 375 CCAGATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 2533 AACGTT AA^CGTT
RcaI 1310 TCATGA T^CATGA
RsrII 2731 CGGTCCG CG^G[AT]CCG
SacI 3144 GAGCTC GAGCT^C
SacII 2621 CCGCGG CCGC^GG
SapI 1314 GAAGAGC GCTCTTC(1/4)
TaqI 257 TCGA T^CGA
TthHB8I 257 TCGA T^CGA
Van91I 375 CCAGATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccI 286 GTAGAC GT^[AC][GT]AC
1951 GTAGAC
AccIII 525 TCCGGA T^CCGGA
879 TCCGGA
AcyI 222 GGCGTC G[GA]^CG[CT]C
2806 GGCGTC
AfaI 653 GTAC GT^AC
1213 GTAC
AocI 1285 CCTCAGG CC^T[ACGT]AGG
1628 CCTCAGG
AspHI 2317 GTGCTC G[AT]GC[AT]^C
3144 GAGCTC
AspI 219 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2223 GACGCAGTC
BbsI 837 GAAGAC GAAGAC(2/6)
2442 GAAGAC
BclI 1001 TGATCA T^GATCA
2789 TGATCA
BglI 2360 GCCTCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2721 GCCTCCCAGGC
BpuAI 837 GAAGAC GAAGAC(2/6)
2442 GAAGAC
BsaBI 2772 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2790 GATCACTATC
BsaHI 222 GGCGTC G[GA]^CG[CT]C
2806 GGCGTC
BseAI 525 TCCGGA T^CCGGA
879 TCCGGA
BsiHKAI 2317 GTGCTC G[AT]GC[AT]^C
3144 GAGCTC
BspEI 525 TCCGGA T^CCGGA
879 TCCGGA
BspMI 805 ACCTGC ACCTGC(4/8)
507 GCAGGT
BsrDI 1400 CATTGC GCAATG(2/0)
41 GCAATG
BsrFI 49 GCCGGC [GA]^CCGG[CT]
2515 ACCGGC
BssSI 70 CACGAG CACGAG(-5/-1)
166 CACGAG
BstXI 122 CCAGTCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3230 CCACCCGTGTGG
Bsu36I 1285 CCTCAGG CC^T[ACGT]AGG
1628 CCTCAGG
Cfr10I 49 GCCGGC [GA]^CCGG[CT]
2515 ACCGGC
EarI 529 GAAGAG CTCTTC(1/4)
1314 GAAGAG
Eco81I 1285 CCTCAGG CC^T[ACGT]AGG
1628 CCTCAGG
EcoNI 205 CCTGCCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1320 CCTGCCCCAGG
EcoT22I 2146 ATGCAT ATGCA^T
3523 ATGCAT
FbaI 1001 TGATCA T^GATCA
2789 TGATCA
Hin1I 222 GGCGTC G[GA]^CG[CT]C
2806 GGCGTC
Ksp632I 529 GAAGAG CTCTTC(1/4)
1314 GAAGAG
MamI 2772 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2790 GATCACTATC
MroI 525 TCCGGA T^CCGGA
879 TCCGGA
MseI 1859 TTAA T^TAA
3380 TTAA
NsiI 2146 ATGCAT ATGCA^T
3523 ATGCAT
PflFI 219 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2223 GACGCAGTC
PpuMI 675 GGGTCCT [GA]G^G[AT]CC[CT]
1772 GGGTCCC
PshAI 611 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2128 GACACCTGTC
RsaI 653 GTAC GT^AC
1213 GTAC
SmlI 175 CTCAAG C^T[CT][GA]AG
1543 CTCAAG
Tru9I 1859 TTAA T^TAA
3380 TTAA
Tth111I 219 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2223 GACGCAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AflIII AluI AlwI
AlwNI ApaI AvaI AvaII BalI
BanI BanII BbrPI BbvCI BbvI
BcgI BcnI BfaI BmyI BpmI
BsaAI BsaI BsaJI BsaWI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PmaCI PmlI PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StuI StyI TseI
Tsp45I TspRI XcmI XhoII XmaCI
XmaI