Restriction map for
pF1KB8397
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspHI AspI AvaI
AviII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsiEI BsiHKAI BsiWI
BsiYI BslI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HphI KasI KpnI KspI
MamI MfeI MluI MslI MspA1I
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
StuI SwaI Tsp45I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 979 GTAGAC GT^[AC][GT]AC
AccIII 790 TCCGGA T^CCGGA
AflIII 742 ACACGT A^C[GA][CT]GT
Asp700I 909 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 993 CCTAGG C^CTAGG
BalI 846 TGGCCA TGG^CCA
BamHI 1149 GGATCC G^GATCC
BanI 939 GGCACC G^G[CT][GA]CC
BanII 1135 GGGCTC G[GA]GC[CT]^C
BbvI 898 GCAGC GCAGC(8/12)
BlnI 993 CCTAGG C^CTAGG
BmyI 1135 GGGCTC G[AGT]GC[ACT]^C
BsaWI 790 TCCGGA [AT]^CCGG[AT]
BseAI 790 TCCGGA T^CCGGA
BseRI 340 GAGGAG GAGGAG(10/8)
BsgI 755 GTGCAG GTGCAG(16/14)
Bsp1286I 1135 GGGCTC G[AGT]GC[ACT]^C
BspEI 790 TCCGGA T^CCGGA
BsrBI 718 GAGCGG CCGCTC(-3/-3)
BsrDI 1124 CATTGC GCAATG(2/0)
BstEII 1028 GGTTACC G^GT[ACGT]ACC
BstPI 1028 GGTTACC G^GT[ACGT]ACC
BstYI 1149 GGATCC [GA]^GATC[CT]
DraI 1264 TTTAAA TTT^AAA
DraII 989 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 989 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1028 GGTTACC G^GT[ACGT]ACC
FauI 720 GCGGG CCCGC(4/6)
Fnu4HI 898 GCAGC GC^[ACGT]GC
HapII 791 CCGG C^CGG
HindIII 1249 AAGCTT A^AGCTT
HpaII 791 CCGG C^CGG
ItaI 898 GCAGC GC^[ACGT]GC
MaeII 744 ACGT A^CGT
MflI 1149 GGATCC [GA]^GATC[CT]
MluNI 846 TGGCCA TGG^CCA
MroI 790 TCCGGA T^CCGGA
MscI 846 TGGCCA TGG^CCA
MspI 791 CCGG C^CGG
NspI 1373 GCATGC [GA]CATG^[CT]
PstI 325 CTGCAG CTGCA^G
SphI 1373 GCATGC GCATG^C
TseI 898 GCAGC G^C[AT]GC
XhoII 1149 GGATCC [GA]^GATC[CT]
XmnI 909 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 738 CCGC CCGC(-3/-1)
720 GCGG
AcsI 1090 AAATTT [GA]^AATT[CT]
1312 GAATTT
AfaI 804 GTAC GT^AC
1255 GTAC
ApoI 1090 AAATTT [GA]^AATT[CT]
1312 GAATTT
AseI 1068 ATTAAT AT^TAAT
1074 ATTAAT
AsnI 1068 ATTAAT AT^TAAT
1074 ATTAAT
BssKI 306 CCAGG ^CC[ACGT]GG
1044 CCAGG
BstNI 306 CCAGG CC^[AT]GG
1044 CCAGG
Cac8I 692 GCGAGC GC[ACGT]^[ACGT]GC
1373 GCATGC
EaeI 846 TGGCCA [CT]^GGCC[GA]
969 TGGCCG
EcoRII 306 CCAGG ^CC[AT]GG
1044 CCAGG
HinfI 531 GATTC G^A[ACGT]TC
825 GAATC
MvaI 306 CCAGG CC^[AT]GG
1044 CCAGG
MwoI 301 GCTATCCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1128 GCTTTCAGGGC
PshBI 1068 ATTAAT AT^TAAT
1074 ATTAAT
RsaI 804 GTAC GT^AC
1255 GTAC
ScrFI 306 CCAGG CC^[ACGT]GG
1044 CCAGG
SfcI 311 CTGTAG C^T[GA][CT]AG
325 CTGCAG
SspI 635 AATATT AAT^ATT
1071 AATATT
TfiI 531 GATTC G^A[AT]TC
825 GAATC
TspRI 164 CAGTG CA[GC]TG[ACGT][ACGT]^
232 CAGTG
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AluI AlwI AvaII BbsI BfaI
BpmI BpuAI BsaJI BsmAI BsrI
BstF5I Cfr13I DdeI DpnII EarI
EcoT14I FokI HaeIII Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
NdeII NlaIII NlaIV Sau3AI Sau96I
SfaNI SmlI StyI TaqI Tru9I
Tsp509I TthHB8I