Restriction map for
pF1KB7638
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BbeI BbsI BciVI BclI
BfrI BglII BlnI BpuAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MseI
MslI MunI MvnI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tru9I Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 671 GTCGAC GT^[AC][GT]AC
AcsI 419 AAATTT [GA]^AATT[CT]
AfaI 996 GTAC GT^AC
AhdI 131 GACGGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 419 AAATTT [GA]^AATT[CT]
AspEI 131 GACGGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 850 GAGCAC G[AT]GC[AT]^C
BamHI 664 GGATCC G^GATCC
BanI 1022 GGCACC G^G[CT][GA]CC
BbrPI 680 CACGTG CAC^GTG
BbvCI 685 GCTGAGG CCTCAGC(-5/-2)
BfaI 805 CTAG C^TAG
BlpI 360 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 360 GCTAAGC GC^T[ACGT]AGC
BsaAI 680 CACGTG [CT]AC^GT[GA]
BsgI 205 CTGCAC GTGCAG(16/14)
BsiHKAI 850 GAGCAC G[AT]GC[AT]^C
BspMI 1011 GCAGGT ACCTGC(4/8)
BsrFI 53 GCCGGC [GA]^CCGG[CT]
BstAPI 301 GCACTGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 399 GGATG GGATG(2/0)
BstYI 664 GGATCC [GA]^GATC[CT]
CelII 360 GCTAAGC GC^T[ACGT]AGC
CfoI 793 GCGC GCG^C
Cfr10I 53 GCCGGC [GA]^CCGG[CT]
Eam1105I 131 GACGGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FokI 399 GGATG GGATG(9/13)
HhaI 793 GCGC GCG^C
HinP1I 793 GCGC G^CGC
HincII 671 GTCGAC GT[CT]^[GA]AC
HindII 671 GTCGAC GT[CT]^[GA]AC
MaeI 805 CTAG C^TAG
MflI 664 GGATCC [GA]^GATC[CT]
NaeI 53 GCCGGC GCC^GGC
NgoMIV 53 GCCGGC G^CCGGC
PflMI 417 CCAAATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 586 GAGTC GAGTC(4/5)
PmaCI 680 CACGTG CAC^GTG
PmlI 680 CACGTG CAC^GTG
RsaI 996 GTAC GT^AC
SalI 671 GTCGAC G^TCGAC
SphI 306 GCATGC GCATG^C
StuI 171 AGGCCT AGG^CCT
Tsp45I 1096 GTGAC ^GT[GC]AC
Tsp509I 420 AATT ^AATT
Van91I 417 CCAAATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 664 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 665 GATCC GGATC(4/5)
664 GGATC
AlwNI 876 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1111 CAGGCCCTG
BalI 414 TGGCCA TGG^CCA
953 TGGCCA
BcgI 177 CGATGTAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
228 CGATGCCCCTGC
BglI 784 GCCAGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
946 GCCCTGGTGGC
BsaI 406 GAGACC GGTCTC(1/5)
1063 GAGACC
BsrBI 484 GAGCGG CCGCTC(-3/-3)
863 GAGCGG
DpnII 607 GATC ^GATC
665 GATC
EaeI 414 TGGCCA [CT]^GGCC[GA]
953 TGGCCA
EcoNI 913 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1104 CCTTTCTCAGG
MaeII 102 ACGT A^CGT
681 ACGT
MboI 607 GATC ^GATC
665 GATC
MluNI 414 TGGCCA TGG^CCA
953 TGGCCA
MscI 414 TGGCCA TGG^CCA
953 TGGCCA
NdeII 607 GATC ^GATC
665 GATC
NspI 306 GCATGC [GA]CATG^[CT]
624 ACATGC
PpuMI 432 GGGACCC [GA]G^G[AT]CC[CT]
923 GGGACCC
Sau3AI 607 GATC ^GATC
665 GATC
SmaI 617 CCCGGG CCC^GGG
749 CCCGGG
SmlI 999 CTTGAG C^T[CT][GA]AG
1138 CTCAAG
TaqI 176 TCGA T^CGA
672 TCGA
TthHB8I 176 TCGA T^CGA
672 TCGA
XcmI 59 CCAAGAGTTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
279 CCAGGACCGCCATGG
XmaCI 617 CCCGGG C^CCGGG
749 CCCGGG
XmaI 617 CCCGGG C^CCGGG
749 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI ApaI AvaI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BssKI BstNI
Cac8I Cfr13I DdeI DraII DsaI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeIII MboII
MnlI MspA1I MspI MvaI MwoI
NciI NcoI NlaIII NlaIV PstI
SapI Sau96I ScrFI SfaNI SfcI
StyI TfiI TseI TspRI