Restriction map for
pF1KB8390
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AgeI AhdI Alw44I AlwI AlwNI
AocI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvrII BalI BamHI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaI BseRI
BsgI BsiHKAI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrGI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KpnI MaeI MamI MfeI MflI
MluI MluNI MscI MslI MunI
NcoI NdeI NheI NsiI NspV
PacI PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI ScaI SexAI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI SwaI TfiI
Tsp509I Tth111I Van91I XbaI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 927 GACGTC GACGT^C
AccIII 20 TCCGGA T^CCGGA
AflIII 887 ACGTGT A^C[GA][CT]GT
Asp700I 1002 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 1156 GCTGAGG CCTCAGC(-5/-2)
BsaWI 20 TCCGGA [AT]^CCGG[AT]
BseAI 20 TCCGGA T^CCGGA
BspEI 20 TCCGGA T^CCGGA
BsrDI 101 CATTGC GCAATG(2/0)
BssHII 425 GCGCGC G^CGCGC
BssSI 325 CACGAG CACGAG(-5/-1)
DdeI 1157 CTGAG C^T[ACGT]AG
DpnII 759 GATC ^GATC
EcoT14I 779 CCAAGG C^C[AT][AT]GG
KspI 5 CCGCGG CCGC^GG
MaeIII 534 GTCAC ^GT[ACGT]AC
MboI 759 GATC ^GATC
MroI 20 TCCGGA T^CCGGA
MseI 1169 TTAA T^TAA
NaeI 231 GCCGGC GCC^GGC
NdeII 759 GATC ^GATC
NgoMIV 231 GCCGGC G^CCGGC
NruI 1112 TCGCGA TCG^CGA
NspI 227 ACATGC [GA]CATG^[CT]
PaeR7I 396 CTCGAG C^TCGAG
PpuMI 1187 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 420 CAGCTG CAG^CTG
SacII 5 CCGCGG CCGC^GG
Sau3AI 759 GATC ^GATC
SbfI 162 CCTGCAGG CCTGCA^GG
SfiI 3 GGCCGCGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 396 CTCGAG C^T[CT][GA]AG
Sse8387I 162 CCTGCAGG CCTGCA^GG
StyI 779 CCAAGG C^C[AT][AT]GG
TaqI 397 TCGA T^CGA
Tru9I 1169 TTAA T^TAA
Tsp45I 534 GTCAC ^GT[GC]AC
TspRI 251 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 397 TCGA T^CGA
XcmI 779 CCAAGGCCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 396 CTCGAG C^TCGAG
XmnI 1002 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Aor51HI 1061 AGCGCT AGC^GCT
1088 AGCGCT
ApaI 644 GGGCCC GGGCC^C
726 GGGCCC
BbrPI 886 CACGTG CAC^GTG
895 CACGTG
BbsI 291 GAAGAC GAAGAC(2/6)
924 GAAGAC
BpmI 46 CTCCAG CTGGAG(16/14)
198 CTCCAG
BpuAI 291 GAAGAC GAAGAC(2/6)
924 GAAGAC
BsiEI 677 CGGCCG CG[GA][CT]^CG
692 CGGCCG
BsrFI 231 GCCGGC [GA]^CCGG[CT]
378 GCCGGT
BsrI 986 ACTGG ACTGG(1/-1)
528 CCAGT
BstF5I 265 CATCC GGATG(2/0)
573 CATCC
Cfr10I 231 GCCGGC [GA]^CCGG[CT]
378 GCCGGT
EagI 677 CGGCCG C^GGCCG
692 CGGCCG
EclXI 677 CGGCCG C^GGCCG
692 CGGCCG
Eco47III 1061 AGCGCT AGC^GCT
1088 AGCGCT
Eco52I 677 CGGCCG C^GGCCG
692 CGGCCG
FokI 265 CATCC GGATG(9/13)
573 CATCC
NotI 676 GCGGCCGC GC^GGCCGC
691 GCGGCCGC
PmaCI 886 CACGTG CAC^GTG
895 CACGTG
PmlI 886 CACGTG CAC^GTG
895 CACGTG
StuI 38 AGGCCT AGG^CCT
714 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI AvaII AviII BanI BanII
BbeI BbvI BcnI BglI BmyI
BsaAI BsaHI BsaJI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DraII DsaI EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeII MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NlaIII NlaIV PleI
PstI RsaI SapI Sau96I ScrFI
SfaNI SfcI SfoI TseI