Restriction map for
pF1KB8389
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# NO CUT ENZYMES ( 122 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BseAI BsgI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrFI BsrGI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DrdI Eam1105I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
MaeII MamI MfeI MluNI MroI
MscI MslI MunI NaeI NcoI
NdeI NgoMIV NotI NruI NsiI
NspI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SbfI ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tsp45I Tth111I XbaI
XcmI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcyI 1043 GGCGCC G[GA]^CG[CT]C
AfaI 1026 GTAC GT^AC
AflIII 827 ACGCGT A^C[GA][CT]GT
Aor51HI 965 AGCGCT AGC^GCT
AvaII 259 GGTCC G^G[AT]CC
BamHI 1145 GGATCC G^GATCC
BbeI 1043 GGCGCC GGCGC^C
BsaHI 1043 GGCGCC G[GA]^CG[CT]C
BsaWI 1142 ACCGGA [AT]^CCGG[AT]
BsmAI 490 GTCTC GTCTC(1/5)
BsmFI 260 GTCCC GGGAC(10/14)
BsrBI 1039 GAGCGG CCGCTC(-3/-3)
BsrDI 212 GCAATG GCAATG(2/0)
BssHII 644 GCGCGC G^CGCGC
BssSI 414 CTCGTG CACGAG(-5/-1)
BstYI 1145 GGATCC [GA]^GATC[CT]
DdeI 599 CTCAG C^T[ACGT]AG
DraIII 411 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 965 AGCGCT AGC^GCT
EcoNI 594 CCTTTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 617 CCAAGG C^C[AT][AT]GG
Hin1I 1043 GGCGCC G[GA]^CG[CT]C
HincII 40 GTCGAC GT[CT]^[GA]AC
HindII 40 GTCGAC GT[CT]^[GA]AC
HphI 919 GGTGA GGTGA(8/7)
KasI 1043 GGCGCC G^GCGCC
MaeIII 116 GTTAC ^GT[ACGT]AC
MflI 1145 GGATCC [GA]^GATC[CT]
MluI 827 ACGCGT A^CGCGT
MseI 1169 TTAA T^TAA
NarI 1043 GGCGCC GG^CGCC
NheI 714 GCTAGC G^CTAGC
NlaIII 5 CATG CATG^
PaeR7I 1034 CTCGAG C^TCGAG
PpuMI 258 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 172 CTGCAG CTGCA^G
RsaI 1026 GTAC GT^AC
SalI 40 GTCGAC G^TCGAC
SapI 186 GCTCTTC GCTCTTC(1/4)
SfaNI 733 GATGC GCATC(5/9)
SfcI 172 CTGCAG C^T[GA][CT]AG
SfiI 729 GGCCGATGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1043 GGCGCC GGC^GCC
SmlI 1034 CTCGAG C^T[CT][GA]AG
StuI 602 AGGCCT AGG^CCT
StyI 617 CCAAGG C^C[AT][AT]GG
Tru9I 1169 TTAA T^TAA
XhoI 1034 CTCGAG C^TCGAG
XhoII 1145 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccI 40 GTCGAC GT^[AC][GT]AC
136 GTCTAC
AlwI 1146 GATCC GGATC(4/5)
1145 GGATC
AlwNI 575 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
988 CAGACACTG
ApaI 280 GGGCCC GGGCC^C
528 GGGCCC
BseRI 817 GAGGAG GAGGAG(10/8)
847 GAGGAG
BsiEI 728 CGGCCG CG[GA][CT]^CG
839 CGGCCG
BspMI 140 ACCTGC ACCTGC(4/8)
158 ACCTGC
BstF5I 326 GGATG GGATG(2/0)
1112 GGATG
DpnII 700 GATC ^GATC
1146 GATC
EagI 728 CGGCCG C^GGCCG
839 CGGCCG
EarI 187 CTCTTC CTCTTC(1/4)
442 GAAGAG
EclXI 728 CGGCCG C^GGCCG
839 CGGCCG
Eco52I 728 CGGCCG C^GGCCG
839 CGGCCG
FokI 326 GGATG GGATG(9/13)
1112 GGATG
Ksp632I 187 CTCTTC CTCTTC(1/4)
442 GAAGAG
KspI 523 CCGCGG CCGC^GG
842 CCGCGG
MboI 700 GATC ^GATC
1146 GATC
NdeII 700 GATC ^GATC
1146 GATC
PflMI 574 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
987 CCAGACACTGG
PleI 43 GACTC GAGTC(4/5)
466 GACTC
SacII 523 CCGCGG CCGC^GG
842 CCGCGG
Sau3AI 700 GATC ^GATC
1146 GATC
SmaI 262 CCCGGG CCC^GGG
684 CCCGGG
Tsp509I 910 AATT ^AATT
1171 AATT
TspRI 575 CAGTG CA[GC]TG[ACGT][ACGT]^
992 CACTG
Van91I 574 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
987 CCAGACACTGG
XmaCI 262 CCCGGG C^CCGGG
684 CCCGGG
XmaI 262 CCCGGG C^CCGGG
684 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AluI AspHI AvaI
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsoBI Bsp1286I BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII ItaI MaeI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIV SacI Sau96I ScrFI
TaqI TfiI TseI TthHB8I