Restriction map for
pF1KB8385
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI Alw44I AocI Aor51HI
ApaLI ApoI AseI AsnI Asp718I
AvrII BalI BamHI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaI BsaWI BseAI BsiWI
BsmAI BsmBI Bsp1407I BspDI BspEI
BspHI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI HindIII
HpaI KpnI MaeI MamI MfeI
MluI MluNI MroI MscI MslI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
ScaI SfiI SfuI SnaBI SpeI
SspBI SspI StuI StyI SwaI
TfiI Tsp45I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 940 GTCGAC GT^[AC][GT]AC
AhdI 739 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 375 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AscI 139 GGCGCGCC GG^CGCGCC
Asp700I 460 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 739 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 868 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 814 CACGTG CAC^GTG
BglI 1094 GCCGTTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 811 CTGCAC GTGCAG(16/14)
BsiEI 867 CGACCG CG[GA][CT]^CG
BsmI 479 GAATGC GAATGC(1/-1)
BspLU11I 1250 ACATGT A^CATGT
BsrBI 279 GAGCGG CCGCTC(-3/-3)
BsrDI 4 GCAATG GCAATG(2/0)
DdeI 945 CTCAG C^T[ACGT]AG
DsaI 90 CCGCGG C^C[GA][CT]GG
Eam1105I 739 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 940 GTCGAC GT[CT]^[GA]AC
HindII 940 GTCGAC GT[CT]^[GA]AC
HinfI 943 GACTC G^A[ACGT]TC
KspI 90 CCGCGG CCGC^GG
MaeIII 1218 GTTAC ^GT[ACGT]AC
MseI 352 TTAA T^TAA
PflFI 868 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 943 GACTC GAGTC(4/5)
PmaCI 814 CACGTG CAC^GTG
PmlI 814 CACGTG CAC^GTG
PpuMI 527 GGGACCC [GA]G^G[AT]CC[CT]
SacII 90 CCGCGG CCGC^GG
SalI 940 GTCGAC G^TCGAC
SbfI 1029 CCTGCAGG CCTGCA^GG
SexAI 740 ACCAGGT A^CC[AT]GGT
SgrAI 108 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 162 CCCGGG CCC^GGG
Sse8387I 1029 CCTGCAGG CCTGCA^GG
Tru9I 352 TTAA T^TAA
Tsp509I 299 AATT ^AATT
Tth111I 868 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 629 CCAACAACATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 162 CCCGGG C^CCGGG
XmaI 162 CCCGGG C^CCGGG
XmnI 460 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AflIII 936 ACACGT A^C[GA][CT]GT
1250 ACATGT
AlwI 28 GATCC GGATC(4/5)
723 GGATC
ApaI 216 GGGCCC GGGCC^C
698 GGGCCC
AvaI 135 CTCGGG C^[CT]CG[GA]G
162 CCCGGG
AvaII 528 GGACC G^G[AT]CC
888 GGACC
AviII 1048 TGCGCA TGC^GCA
1130 TGCGCA
BanI 106 GGCGCC G^G[CT][GA]CC
819 GGCGCC
BbeI 106 GGCGCC GGCGC^C
819 GGCGCC
BpmI 765 CTGGAG CTGGAG(16/14)
1054 CTGGAG
BsaAI 814 CACGTG [CT]AC^GT[GA]
1063 TACGTG
BsoBI 135 CTCGGG C^[CT]CG[GA]G
162 CCCGGG
BspMI 304 ACCTGC ACCTGC(4/8)
912 GCAGGT
BsrFI 109 GCCGGC [GA]^CCGG[CT]
838 GCCGGC
BstAPI 404 GCAACCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
611 GCAGCCAGTGC
BstYI 27 AGATCC [GA]^GATC[CT]
723 GGATCT
Cfr10I 109 GCCGGC [GA]^CCGG[CT]
838 GCCGGC
DraII 215 AGGGCCC [GA]G^G[ACGT]CC[CT]
527 GGGACCC
EarI 679 CTCTTC CTCTTC(1/4)
1162 CTCTTC
EcoO109I 215 AGGGCCC [GA]G^G[ACGT]CC[CT]
527 GGGACCC
FspI 1048 TGCGCA TGC^GCA
1130 TGCGCA
KasI 106 GGCGCC G^GCGCC
819 GGCGCC
Ksp632I 679 CTCTTC CTCTTC(1/4)
1162 CTCTTC
MflI 27 AGATCC [GA]^GATC[CT]
723 GGATCT
NaeI 109 GCCGGC GCC^GGC
838 GCCGGC
NarI 106 GGCGCC GG^CGCC
819 GGCGCC
NgoMIV 109 GCCGGC G^CCGGC
838 GCCGGC
PstI 727 CTGCAG CTGCA^G
1030 CTGCAG
PvuII 18 CAGCTG CAG^CTG
957 CAGCTG
SapI 678 GCTCTTC GCTCTTC(1/4)
1161 GCTCTTC
SfcI 727 CTGCAG C^T[GA][CT]AG
1030 CTGCAG
SfoI 106 GGCGCC GGC^GCC
819 GGCGCC
SmlI 427 CTCAAG C^T[CT][GA]AG
925 CTCAAG
SphI 620 GCATGC GCATG^C
846 GCATGC
XhoII 27 AGATCC [GA]^GATC[CT]
723 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI BanII BbvI BcnI BmyI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BsrI BssHII
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DpnII EaeI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI MaeII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TseI TspRI
TthHB8I