Restriction map for
pF1KB8378
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BlnI BsaBI
BsiWI BsmI BspDI BspHI BspLU11I
BsrDI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DraI Eam1105I
Eco47III EcoNI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
MaeI MamI MfeI MseI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI TfiI Tru9I XbaI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccIII 194 TCCGGA T^CCGGA
AcsI 730 GAATTC [GA]^AATT[CT]
AflIII 1263 ACGCGT A^C[GA][CT]GT
Alw44I 342 GTGCAC G^TGCAC
AocI 1147 CCTGAGG CC^T[ACGT]AGG
ApaI 292 GGGCCC GGGCC^C
ApaLI 342 GTGCAC G^TGCAC
ApoI 730 GAATTC [GA]^AATT[CT]
AspI 1459 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1097 GGATCC G^GATCC
BbeI 1239 GGCGCC GGCGC^C
BbsI 226 GTCTTC GAAGAC(2/6)
BglII 1040 AGATCT A^GATCT
BlpI 81 GCTCAGC GC^T[ACGT]AGC
BpmI 535 CTGGAG CTGGAG(16/14)
Bpu1102I 81 GCTCAGC GC^T[ACGT]AGC
BpuAI 226 GTCTTC GAAGAC(2/6)
BsaAI 466 TACGTG [CT]AC^GT[GA]
BsaI 956 GAGACC GGTCTC(1/5)
BsaWI 194 TCCGGA [AT]^CCGG[AT]
BseAI 194 TCCGGA T^CCGGA
BsiEI 1274 CGGCCG CG[GA][CT]^CG
BsmBI 819 GAGACG CGTCTC(1/5)
Bsp1407I 578 TGTACA T^GTACA
BspEI 194 TCCGGA T^CCGGA
BsrBI 112 CCGCTC CCGCTC(-3/-3)
BsrFI 89 GCCGGC [GA]^CCGG[CT]
BsrGI 578 TGTACA T^GTACA
Bsu36I 1147 CCTGAGG CC^T[ACGT]AGG
CelII 81 GCTCAGC GC^T[ACGT]AGC
Cfr10I 89 GCCGGC [GA]^CCGG[CT]
EagI 1274 CGGCCG C^GGCCG
EclXI 1274 CGGCCG C^GGCCG
Eco52I 1274 CGGCCG C^GGCCG
Eco81I 1147 CCTGAGG CC^T[ACGT]AGG
EcoRI 730 GAATTC G^AATTC
HincII 550 GTCAAC GT[CT]^[GA]AC
HindII 550 GTCAAC GT[CT]^[GA]AC
HinfI 700 GACTC G^A[ACGT]TC
KasI 1239 GGCGCC G^GCGCC
KspI 961 CCGCGG CCGC^GG
MluI 1263 ACGCGT A^CGCGT
MroI 194 TCCGGA T^CCGGA
NaeI 89 GCCGGC GCC^GGC
NarI 1239 GGCGCC GG^CGCC
NgoMIV 89 GCCGGC G^CCGGC
NspI 1436 ACATGC [GA]CATG^[CT]
PaeR7I 704 CTCGAG C^TCGAG
PflFI 1459 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1173 CCAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 700 GACTC GAGTC(4/5)
SacII 961 CCGCGG CCGC^GG
ScaI 1598 AGTACT AGT^ACT
SfoI 1239 GGCGCC GGC^GCC
SmaI 37 CCCGGG CCC^GGG
SspBI 578 TGTACA T^GTACA
Tth111I 1459 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1173 CCAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 704 CTCGAG C^TCGAG
XmaCI 37 CCCGGG C^CCGGG
XmaI 37 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 1239 GGCGCC G[GA]^CG[CT]C
1462 GGCGTC
AlwNI 259 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1441 CAGGTCCTG
BalI 929 TGGCCA TGG^CCA
1556 TGGCCA
BanI 92 GGCACC G^G[CT][GA]CC
1239 GGCGCC
BglI 1607 GCCACAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1645 GCCGGAGAGGC
BsaHI 1239 GGCGCC G[GA]^CG[CT]C
1462 GGCGTC
BstEII 375 GGTGACC G^GT[ACGT]ACC
1338 GGTGACC
BstPI 375 GGTGACC G^GT[ACGT]ACC
1338 GGTGACC
BstXI 488 CCAGGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1229 CCACCAACGTGG
DraIII 871 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
1478 CACGGGGTG
DrdI 1435 GACATGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1456 GACGACGGCGTC
EarI 511 CTCTTC CTCTTC(1/4)
816 GAAGAG
EcoO65I 375 GGTGACC G^GT[ACGT]ACC
1338 GGTGACC
EcoT14I 221 CCAAGG C^C[AT][AT]GG
1253 CCAAGG
HaeII 1239 GGCGCC [GA]GCGC^[CT]
1551 GGCGCT
HgaI 821 GACGC GACGC(5/10)
1463 GCGTC
Hin1I 1239 GGCGCC G[GA]^CG[CT]C
1462 GGCGTC
Ksp632I 511 CTCTTC CTCTTC(1/4)
816 GAAGAG
MaeII 467 ACGT A^CGT
1235 ACGT
MluNI 929 TGGCCA TGG^CCA
1556 TGGCCA
MscI 929 TGGCCA TGG^CCA
1556 TGGCCA
PstI 145 CTGCAG CTGCA^G
651 CTGCAG
StyI 221 CCAAGG C^C[AT][AT]GG
1253 CCAAGG
Tsp509I 731 AATT ^AATT
1138 AATT
XcmI 1397 CCATCATCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1559 CCAAGTGCGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BanII BbvI
BcnI BmyI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PpuMI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI TaqI TseI Tsp45I TspRI
TthHB8I XhoII