Restriction map for
pF1KB8370
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# NO CUT ENZYMES ( 169 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BmyI BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MaeIII MamI
MfeI MluI MluNI MroI MscI
MseI MspA1I MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp45I Tth111I Van91I XbaI
XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AcsI 61 AAATTC [GA]^AATT[CT]
AlwI 250 GATCC GGATC(4/5)
AlwNI 464 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 61 AAATTC [GA]^AATT[CT]
BanI 312 GGTGCC G^G[CT][GA]CC
BcnI 216 CCCGG CC^[GC]GG
BfaI 338 CTAG C^TAG
BlpI 300 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 300 GCTGAGC GC^T[ACGT]AGC
BsaI 135 GAGACC GGTCTC(1/5)
BspMI 377 ACCTGC ACCTGC(4/8)
BsrBI 52 GAGCGG CCGCTC(-3/-3)
BsrFI 309 GCCGGT [GA]^CCGG[CT]
BstXI 267 CCAGTCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 249 AGATCC [GA]^GATC[CT]
CelII 300 GCTGAGC GC^T[ACGT]AGC
CfoI 30 GCGC GCG^C
Cfr10I 309 GCCGGT [GA]^CCGG[CT]
DdeI 301 CTGAG C^T[ACGT]AG
DpnII 250 GATC ^GATC
DsaI 161 CCATGG C^C[GA][CT]GG
EarI 436 GAAGAG CTCTTC(1/4)
HaeII 29 AGCGCC [GA]GCGC^[CT]
HaeIII 18 GGCC GG^CC
HhaI 30 GCGC GCG^C
HinP1I 30 GCGC G^CGC
HphI 451 GGTGA GGTGA(8/7)
Ksp632I 436 GAAGAG CTCTTC(1/4)
MaeI 338 CTAG C^TAG
MboI 250 GATC ^GATC
MflI 249 AGATCC [GA]^GATC[CT]
MslI 162 CATGGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 216 CCCGG CC^[GC]GG
NcoI 161 CCATGG C^CATGG
NdeII 250 GATC ^GATC
NlaIII 162 CATG CATG^
PaeR7I 124 CTCGAG C^TCGAG
PstI 477 CTGCAG CTGCA^G
Sau3AI 250 GATC ^GATC
SfcI 477 CTGCAG C^T[GA][CT]AG
SmlI 124 CTCGAG C^T[CT][GA]AG
Tsp509I 62 AATT ^AATT
TspRI 182 CACTG CA[GC]TG[ACGT][ACGT]^
XhoI 124 CTCGAG C^TCGAG
XhoII 249 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AluI 38 AGCT AG^CT
299 AGCT
AvaI 14 CTCGGG C^[CT]CG[GA]G
124 CTCGAG
BsmAI 88 GAGAC GTCTC(1/5)
135 GAGAC
BsmFI 212 GGGAC GGGAC(10/14)
111 GTCCC
BsoBI 14 CTCGGG C^[CT]CG[GA]G
124 CTCGAG
BsrI 183 ACTGG ACTGG(1/-1)
267 CCAGT
BstNI 24 CCTGG CC^[AT]GG
469 CCTGG
DraII 212 GGGACCC [GA]G^G[ACGT]CC[CT]
340 AGGACCT
EcoO109I 212 GGGACCC [GA]G^G[ACGT]CC[CT]
340 AGGACCT
EcoRII 24 CCTGG ^CC[AT]GG
469 CCTGG
HapII 217 CCGG C^CGG
310 CCGG
HinfI 127 GAGTC G^A[ACGT]TC
364 GACTC
HpaII 217 CCGG C^CGG
310 CCGG
MspI 217 CCGG C^CGG
310 CCGG
MvaI 24 CCTGG CC^[AT]GG
469 CCTGG
MwoI 30 GCGCCAGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
300 GCTGAGCCTGC
PleI 364 GACTC GAGTC(4/5)
127 GAGTC
PpuMI 212 GGGACCC [GA]G^G[AT]CC[CT]
340 AGGACCT
SfaNI 220 GATGC GCATC(5/9)
120 GCATC
TaqI 68 TCGA T^CGA
125 TCGA
TthHB8I 68 TCGA T^CGA
125 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AvaII BbvI BpmI BsaJI
BseRI BsiYI BslI BssKI BstF5I
Cac8I Cfr13I EcoT14I Fnu4HI FokI
ItaI MboII MnlI NlaIV Sau96I
ScrFI StyI TseI