Restriction map for
pF1KB8367
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I Cfr13I ClaI CpoI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MamI
MfeI MflI MluI MluNI MscI
MspA1I MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 217 GTCTAC GT^[AC][GT]AC
AccIII 281 TCCGGA T^CCGGA
AlwI 284 GGATC GGATC(4/5)
AlwNI 485 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
BbsI 162 GAAGAC GAAGAC(2/6)
BciVI 369 GGATAC GTATCC(6/5)
BfaI 526 CTAG C^TAG
BmyI 335 GTGCCC G[AGT]GC[ACT]^C
BpuAI 162 GAAGAC GAAGAC(2/6)
BsaWI 281 TCCGGA [AT]^CCGG[AT]
BseAI 281 TCCGGA T^CCGGA
BsmAI 208 GTCTC GTCTC(1/5)
BsmI 686 GAATGC GAATGC(1/-1)
Bsp1286I 335 GTGCCC G[AGT]GC[ACT]^C
BspEI 281 TCCGGA T^CCGGA
BspMI 668 GCAGGT ACCTGC(4/8)
CfoI 42 GCGC GCG^C
DraI 247 TTTAAA TTT^AAA
EcoNI 360 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 662 GATATC GAT^ATC
EcoT14I 765 CCTTGG C^C[AT][AT]GG
HapII 282 CCGG C^CGG
HhaI 42 GCGC GCG^C
HinP1I 42 GCGC G^CGC
HpaII 282 CCGG C^CGG
HphI 241 GGTGA GGTGA(8/7)
MaeI 526 CTAG C^TAG
MroI 281 TCCGGA T^CCGGA
MslI 846 CACGACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 282 CCGG C^CGG
PflMI 354 CCAAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 231 GACTC GAGTC(4/5)
SexAI 47 ACCTGGT A^CC[AT]GGT
SspI 632 AATATT AAT^ATT
StuI 74 AGGCCT AGG^CCT
StyI 765 CCTTGG C^C[AT][AT]GG
TaqI 255 TCGA T^CGA
TfiI 480 GAATC G^A[AT]TC
TspRI 624 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 255 TCGA T^CGA
Van91I 354 CCAAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AfaI 126 GTAC GT^AC
421 GTAC
BstF5I 516 CATCC GGATG(2/0)
510 GGATG
Cac8I 145 GCCTGC GC[ACGT]^[ACGT]GC
646 GCCCGC
DpnII 285 GATC ^GATC
700 GATC
FauI 647 CCCGC CCCGC(4/6)
310 GCGGG
FokI 516 CATCC GGATG(9/13)
510 GGATG
HinfI 231 GACTC G^A[ACGT]TC
480 GAATC
MboI 285 GATC ^GATC
700 GATC
NdeII 285 GATC ^GATC
700 GATC
NlaIII 413 CATG CATG^
500 CATG
RsaI 126 GTAC GT^AC
421 GTAC
Sau3AI 285 GATC ^GATC
700 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AluI BbvI BpmI BsaJI
BseRI BsiYI BslI BsrI BssKI
BstNI DdeI EarI EcoRII Fnu4HI
HaeIII ItaI Ksp632I MaeIII MboII
MnlI MseI MvaI MwoI NlaIV
ScrFI SfaNI Tru9I TseI Tsp45I
Tsp509I