Restriction map for
pF1KB8366
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AfaI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanII BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstXI
BstYI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FauI FbaI FokI FseI
FspI HindIII HpaI KpnI KspI
MamI MfeI MflI MluI MluNI
MroI MscI MseI MslI MunI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
TaqI Tru9I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 99 GAATTC [GA]^AATT[CT]
AlwI 72 GATCC GGATC(4/5)
AlwNI 352 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 145 CCTGAGG CC^T[ACGT]AGG
ApoI 99 GAATTC [GA]^AATT[CT]
AvaII 3 GGACC G^G[AT]CC
BbeI 25 GGCGCC GGCGC^C
BmyI 204 GTGCCC G[AGT]GC[ACT]^C
BsaI 140 GAGACC GGTCTC(1/5)
Bsp1286I 204 GTGCCC G[AGT]GC[ACT]^C
Bsu36I 145 CCTGAGG CC^T[ACGT]AGG
Cac8I 28 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 26 GCGC GCG^C
DpnII 72 GATC ^GATC
DraIII 79 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 257 TGGCCG [CT]^GGCC[GA]
Eco81I 145 CCTGAGG CC^T[ACGT]AGG
EcoRI 99 GAATTC G^AATTC
HaeII 25 GGCGCC [GA]GCGC^[CT]
HgaI 267 GACGC GACGC(5/10)
HhaI 26 GCGC GCG^C
HinP1I 26 GCGC G^CGC
HincII 319 GTTGAC GT[CT]^[GA]AC
HindII 319 GTTGAC GT[CT]^[GA]AC
HinfI 354 GATTC G^A[ACGT]TC
KasI 25 GGCGCC G^GCGCC
MaeII 441 ACGT A^CGT
MaeIII 425 GTCAC ^GT[ACGT]AC
MboI 72 GATC ^GATC
MspA1I 21 CAGCGG C[AC]G^C[GT]G
NarI 25 GGCGCC GG^CGCC
NdeII 72 GATC ^GATC
NlaIII 419 CATG CATG^
NspI 418 ACATGC [GA]CATG^[CT]
Sau3AI 72 GATC ^GATC
SfoI 25 GGCGCC GGC^GCC
SspI 526 AATATT AAT^ATT
TfiI 354 GATTC G^A[AT]TC
Tsp45I 425 GTCAC ^GT[GC]AC
Tsp509I 100 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 25 GGCGCC G[GA]^CG[CT]C
267 GACGCC
AluI 31 AGCT AG^CT
162 AGCT
BanI 25 GGCGCC G^G[CT][GA]CC
203 GGTGCC
BbvI 20 GCAGC GCAGC(8/12)
160 GCAGC
BcnI 6 CCGGG CC^[GC]GG
14 CCCGG
BfaI 67 CTAG C^TAG
200 CTAG
BsaHI 25 GGCGCC G[GA]^CG[CT]C
267 GACGCC
BsaJI 59 CCCTGG C^C[ACGT][ACGT]GG
60 CCTGGG
BseRI 130 CTCCTC GAGGAG(10/8)
338 GAGGAG
BsiYI 54 CCTTGCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
246 CCGCTTCGTGG
BslI 54 CCTTGCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
246 CCGCTTCGTGG
BstNI 48 CCTGG CC^[AT]GG
60 CCTGG
DdeI 146 CTGAG C^T[ACGT]AG
334 CTAAG
EarI 431 CTCTTC CTCTTC(1/4)
517 GAAGAG
EcoRII 48 CCTGG ^CC[AT]GG
60 CCTGG
HapII 6 CCGG C^CGG
15 CCGG
Hin1I 25 GGCGCC G[GA]^CG[CT]C
267 GACGCC
HpaII 6 CCGG C^CGG
15 CCGG
HphI 360 GGTGA GGTGA(8/7)
426 TCACC
Ksp632I 431 CTCTTC CTCTTC(1/4)
517 GAAGAG
MaeI 67 CTAG C^TAG
200 CTAG
MspI 6 CCGG C^CGG
15 CCGG
MvaI 48 CCTGG CC^[AT]GG
60 CCTGG
MwoI 23 GCGGCGCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
213 GCAATACAAGC
NciI 6 CCGGG CC^[GC]GG
14 CCCGG
NlaIV 25 GGCGCC GG[ACGT]^[ACGT]CC
203 GGTGCC
SfaNI 242 GATGC GCATC(5/9)
230 GCATC
TseI 20 GCAGC G^C[AT]GC
160 GCAGC
TspRI 41 CACTG CA[GC]TG[ACGT][ACGT]^
79 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
AciI BsgI BsmAI BssKI Cfr13I
Fnu4HI HaeIII ItaI MboII MnlI
Sau96I ScrFI