Restriction map for
pF1KB8365
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AocI
ApoI AseI AsnI Asp718I AspEI
AspI BamHI BbrPI BciVI BclI
BfrI BsaAI BsaBI BsaI BsaWI
BseAI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspMI BsrDI
BsrGI Bst1107I BstAPI BstBI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII Eam1105I Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MluI MroI MseI MunI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tsp509I
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 35 enzymes) #
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Alw44I 937 GTGCAC G^TGCAC
AlwI 596 GGATC GGATC(4/5)
ApaLI 937 GTGCAC G^TGCAC
AscI 964 GGCGCGCC GG^CGCGCC
Asp700I 789 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 937 GTGCAC G[AT]GC[AT]^C
AviII 351 TGCGCA TGC^GCA
AvrII 216 CCTAGG C^CTAGG
BalI 542 TGGCCA TGG^CCA
BbvCI 1355 CCTCAGC CCTCAGC(-5/-2)
BcgI 752 GCAACATGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 584 AGATCT A^GATCT
BlnI 216 CCTAGG C^CTAGG
BsiHKAI 937 GTGCAC G[AT]GC[AT]^C
BsiWI 425 CGTACG C^GTACG
BssHII 965 GCGCGC G^CGCGC
BssSI 1144 CACGAG CACGAG(-5/-1)
BstEII 1329 GGTGACC G^GT[ACGT]ACC
BstPI 1329 GGTGACC G^GT[ACGT]ACC
BstYI 584 AGATCT [GA]^GATC[CT]
EarI 607 CTCTTC CTCTTC(1/4)
EcoO65I 1329 GGTGACC G^GT[ACGT]ACC
FseI 222 GGCCGGCC GGCCGG^CC
FspI 351 TGCGCA TGC^GCA
Ksp632I 607 CTCTTC CTCTTC(1/4)
MflI 584 AGATCT [GA]^GATC[CT]
MluNI 542 TGGCCA TGG^CCA
MscI 542 TGGCCA TGG^CCA
NotI 391 GCGGCCGC GC^GGCCGC
PvuII 1153 CAGCTG CAG^CTG
SfaNI 567 GATGC GCATC(5/9)
SphI 251 GCATGC GCATG^C
XcmI 368 CCATGAATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 584 AGATCT [GA]^GATC[CT]
XmnI 789 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflIII 194 ACATGT A^C[GA][CT]GT
755 ACATGT
Aor51HI 635 AGCGCT AGC^GCT
791 AGCGCT
BbsI 945 GAAGAC GAAGAC(2/6)
1047 GAAGAC
BglI 326 GCCCGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
452 GCCGCGCGGGC
BlpI 321 GCTGAGC GC^T[ACGT]AGC
576 GCTGAGC
BpmI 1052 CTCCAG CTGGAG(16/14)
1363 CTGGAG
Bpu1102I 321 GCTGAGC GC^T[ACGT]AGC
576 GCTGAGC
BpuAI 945 GAAGAC GAAGAC(2/6)
1047 GAAGAC
BsgI 736 CTGCAC GTGCAG(16/14)
1156 CTGCAC
BsiEI 293 CGGCCG CG[GA][CT]^CG
392 CGGCCG
BspLU11I 194 ACATGT A^CATGT
755 ACATGT
BstF5I 546 CATCC GGATG(2/0)
651 CATCC
CelII 321 GCTGAGC GC^T[ACGT]AGC
576 GCTGAGC
DpnII 585 GATC ^GATC
597 GATC
DrdI 177 GACCCCGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
676 GACTGCTTCGTC
EagI 293 CGGCCG C^GGCCG
392 CGGCCG
EclXI 293 CGGCCG C^GGCCG
392 CGGCCG
Eco47III 635 AGCGCT AGC^GCT
791 AGCGCT
Eco52I 293 CGGCCG C^GGCCG
392 CGGCCG
FokI 546 CATCC GGATG(9/13)
651 CATCC
MaeII 481 ACGT A^CGT
1294 ACGT
MaeIII 301 GTGAC ^GT[ACGT]AC
1330 GTGAC
MboI 585 GATC ^GATC
597 GATC
MspA1I 852 CAGCGG C[AC]G^C[GT]G
1153 CAGCTG
NdeII 585 GATC ^GATC
597 GATC
PflMI 35 CCAGCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
996 CCAGACTCTGG
PpuMI 884 AGGACCC [GA]G^G[AT]CC[CT]
1169 GGGACCC
Sau3AI 585 GATC ^GATC
597 GATC
StuI 71 AGGCCT AGG^CCT
108 AGGCCT
TaqI 761 TCGA T^CGA
1349 TCGA
Tsp45I 301 GTGAC ^GT[GC]AC
1330 GTGAC
TthHB8I 761 TCGA T^CGA
1349 TCGA
Van91I 35 CCAGCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
996 CCAGACTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI ApaI AvaI AvaII BanI
BanII BbeI BbvI BcnI BfaI
BmyI BsaHI BsaJI BseRI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
MaeI MboII MnlI MslI MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NgoMIV NlaIII NlaIV
NspI PleI RsaI Sau96I ScrFI
SfoI SmaI StyI TseI TspRI
XmaCI XmaI