##################################
# NO CUT ENZYMES (  191 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        AluI        Alw44I      AlwI        AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaII       AviII       AvrII       BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsgI        BsiEI       BsiWI       BsmAI       
BsmBI       BsmFI       BsmI        Bsp1407I    BspDI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DpnII       
DraI        DraII       DraIII      DrdI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     MaeI        MaeII       MamI        
MboI        MboII       MfeI        MflI        MluI        
MseI        MslI        MunI        NaeI        NarI        
NciI        NdeI        NdeII       NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SalI        SapI        
Sau3AI      Sau96I      SbfI        ScaI        SexAI       
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        TaqI        
Tru9I       Tsp509I     Tth111I     TthHB8I     Van91I      
XbaI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AccII         207  CGCG                 CG^CG
AccIII        289  TCCGGA               T^CCGGA
AlwNI         265  CAGTCACTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         139  GTGCTC               G[AT]GC[AT]^C
AvaI          240  CTCGGG               C^[CT]CG[GA]G
BanII         312  GGGCTC               G[GA]GC[CT]^C
BglI          328  GCCATTGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI         289  TCCGGA               [AT]^CCGG[AT]
BseAI         289  TCCGGA               T^CCGGA
BseRI         116  GAGGAG               GAGGAG(10/8)
BsiHKAI       139  GTGCTC               G[AT]GC[AT]^C
BsiYI          86  CCCTGGCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           86  CCCTGGCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI         240  CTCGGG               C^[CT]CG[GA]G
BspEI         289  TCCGGA               T^CCGGA
BstF5I        306  CATCC                GGATG(2/0)
BstUI         207  CGCG                 CG^CG
DdeI          142  CTCAG                C^T[ACGT]AG
FauI          166  GCGGG                CCCGC(4/6)
FokI          306  CATCC                GGATG(9/13)
HapII         290  CCGG                 C^CGG
HpaII         290  CCGG                 C^CGG
HphI           17  TCACC                GGTGA(8/7)
KspI          206  CCGCGG               CCGC^GG
MroI          289  TCCGGA               T^CCGGA
MspA1I        206  CCGCGG               C[AC]G^C[GT]G
MspI          290  CCGG                 C^CGG
MvnI          207  CGCG                 CG^CG
NlaIV         313  GGCTCC               GG[ACGT]^[ACGT]CC
PleI          284  GACTC                GAGTC(4/5)
PstI          262  CTGCAG               CTGCA^G
SacII         206  CCGCGG               CCGC^GG
StuI            5  AGGCCT               AGG^CCT
TfiI          170  GAATC                G^A[AT]TC
XcmI          299  CCAAGTTCATCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

BalI           41  TGGCCA               TGG^CCA
               89  TGGCCA              
BbvI          190  GCAGC                GCAGC(8/12)
              245  GCAGC               
EcoT14I       134  CCATGG               C^C[AT][AT]GG
              149  CCATGG              
HinfI         170  GAATC                G^A[ACGT]TC
              284  GACTC               
MaeIII         31  GTGAC                ^GT[ACGT]AC
              267  GTCAC               
MluNI          41  TGGCCA               TGG^CCA
               89  TGGCCA              
MscI           41  TGGCCA               TGG^CCA
               89  TGGCCA              
NcoI          134  CCATGG               C^CATGG
              149  CCATGG              
NlaIII        135  CATG                 CATG^
              150  CATG                
SfcI          101  CTACAG               C^T[GA][CT]AG
              262  CTGCAG              
StyI          134  CCATGG               C^C[AT][AT]GG
              149  CCATGG              
TseI          190  GCAGC                G^C[AT]GC
              245  GCAGC               
Tsp45I         31  GTGAC                ^GT[GC]AC
              267  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   19 enzymes) #
#########################################

AciI        BmyI        BsaJI       Bsp1286I    BsrDI       
BsrI        BssKI       BstNI       DsaI        EaeI        
EcoRII      Fnu4HI      HaeIII      ItaI        MnlI        
MvaI        MwoI        ScrFI       TspRI